In Vitro and In Vivo Effect of Poplar Bud Extracts on Phytophthora infestans: A New Effective Biological Method in Potato Late Blight Control

Abstract

The effect of populin extract from black poplar (Populus nigra) on seven different late blight strains was tested under laboratory and field conditions. The growth rate of hyphae was found to be significantly lower in vitro after 3 and 4 v/v% populin applications. Stain M16 was resistant to populin treatment under lab conditions, however. Both 5% and 10% concentration populin reduced the M16 strain’s severity on potato leaves under field conditions and proved to be even more effective than conventionally used fungicides Infinito 687 Sc and Valis M. Higher infection intensity at the 1% level was observed after 24 h using Valis M, and the same trend toward 10% infection remained after 48 and 72 h as well. Low, almost-no-infection intensity was detected after populin 5% and 10% treatment under an open field condition. Altogether, it can be concluded that populin extract can be a low-cost option for growers and an environmentally friendly approach in late blight control.

Anti‐oomycete ability of scopolamine against Phytophthora infestans, a terrible pathogen of potato late blight

“…In the present study, scopolamine and Infinito (6 and 9 μL L −1 ) had additive effects, whereas antagonism was detected when the concentration of Infinito was 3 μL L −1 , meaning that scopolamine could be used together with Infinito at some concentrations. The maximum concentration of the Infinito in vitro experiment was 9 μL L −1 (9 × 10 −6 L L −1 ), which thus far was less than the concentration used in the field trial (4.6 × 10 −3 L L −1 ) 82 . These findings suggest that combinational use has the potential to be put into practical use.…”

“…The maximum concentration of the Infinito in vitro experiment was 9 μL L −1 (9 × 10 −6 L L −1 ), which thus far was less than the concentration used in the field trial (4.6 × 10 −3 L L −1 ). 82 These findings suggest that combinational use has the potential to be put into practical use. This requires further research, such as field trials and cost accounting.…”

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

“…90 Clerck et al 91 evaluated anti-oomycete activities of 90 commercial essential oils from different plants against Phytophthora infestans and found those from Cinnamomum cassia, Cinnamomum flexuosus, Cinnamomum zeylanicum and Mentha pulegium can completely inhibit the growth of this pathogen for at least 120 h at a concentration of 1000 mg/L. As reported by Turóczi et al, 92 populin extracted from black poplar (Populus nigra) inhibits the growth of Phytophthora infestans under laboratory conditions and reduces the severity of late blight on potato leaves under field conditions. Such results suggest that this natural compound has promising application prospects as a biocontrol agent in late blight control.…”

Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease

“…Our findings show that peptide extracts against P. infestans, as determined by optical microscopy, had a high level of inhibition. There are some mentions confirming the application of aquatic and organic plant extracts to suppress P. infestans and other phytopathogenic oomycetes; these are potent agents for biocontrol [ 38 , 39 , 40 , 41 ]. Generally, the target activity is associated with secondary metabolites of a different chemical nature and not with polypeptides.…”