In vitro and in vivo effects of an anti-CD45 monoclonal antibody in the rat

S, Ko; Nakano, Hiroshi; Deiwick, Andrea; Dinkel, Astrid; Wonigeit, K.

doi:10.1016/s0041-1345(99)00221-3

Cited by 3 publications

(1 citation statement)

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“…It was necessary to identify the high expression of IFNγ induced by donor or recipient to prove the explanation reasonable, namely induced by allograft passenger or TH1 cell of recipient. Anti-CD45 Mab with donor specificity was utilized to remove the passengers by Ko, who found the transplantation tolerance induced by DTS was reversed [19] . It proved the possibility of chimerism tolerance from some angle, and indeed more experimental and clinical researches were needed to prove the theory farther.…”

Section: Discussionmentioning

confidence: 99%

Expression of cytokines in acute heart transplantation rejection

Xia

Yang

2006

J. Huazhong Univ. Sc. Technol.

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The expression and changes of local cytokines network were detected in heart transplantation in rats, so as to determine the role of cytokines in the acute rejection of rats of heart transplantation. Allografts were divided into 4 groups (n=12 in each group): group A (control), group B (IL-2 monoclonal antibody-treated), group C (CsA-treated) and group D (IL-2 monoclonal antibody+CsA-treated). Hearts from DA rats were transplanted into a cervical location in Wistar recipients. The local expression of IL-1beta, IL-2, CD25, IL-4, IL-5, IL-6, 1L-10, TNFalpha and INFgamma was detected at day 1, 3, 5, 7, 9, 11 and 14 by reverse transcription polymerase chain reaction. The results showed that the survival time of allografts was 8.3+/-1.7, 29.2+/-7.1 (P<0.05), 26.4+/-5.7 (P<0.05) and 55.0+/-10.6 (P<0.01) days respectively in groups A, B, C and D. The expression of IL-1beta, IL-4, IL-10 and IFNgamma was up-regulated, and that of IL-2, CD25, IL-5, IL-6 and TNFalpha was significantly inhibited in group A; The expression of IL-10, IL-5, IL-6, IL-10 and IFNgamma was up-regulated, and that of IL-2, L-4 and TNFalpha was significantly down-regulated in group B; The expression of IL-1beta, IL-2, CD25, IL-5, TNFalpha and IFNgamma was up-regulated, and that of IL-4, IL-6 and IL-10 was significantly down-regulated in group C; The expression of IL-14, 11-5, IL-6 and 11-10 was up-regulated, and that of IL-10, IL-2, CD25, TNFalpha and IFNgamma was significantly down-regulated in group D. In conclusion, cytokines play an important role in the development of acute transplantation rejection. Different cytokines play different roles in different local environments.

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Section: Discussionmentioning

confidence: 99%

Expression of cytokines in acute heart transplantation rejection

Xia

Yang

2006

J. Huazhong Univ. Sc. Technol.

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Features of tolerance achieved by antigen and a single injection of an anti-CD45 monoclonal antibody in rats

Jäger

Tsui

Aselmann

et al. 2001

Transplantation Proceedings

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The Biology of CD45 and its Use as a Therapeutic Target

Dahlke

Larsen

Rasko

et al. 2004

Leukemia & Lymphoma

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All mature hemopoietic lineage cells, with exclusion of platelets and mature erythrocytes, share the surface expression of a transmembrane phosphatase, the CD45 molecule. It is also present on hemopoietic stem cells and most leukemic clones and therefore presents as an appropriate target for immunotherapy with anti-CD45 antibodies. This short review details the biology of CD45 and its recent targeting for both treatment of malignant disorders and tolerance induction. In particular, the question of potential stem cell depletion for induction of central tolerance or depletion of malignant hemopoietic cells is addressed. Mechanisms underlying the effects downstream of CD45 binding to the cell surface are discussed.

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In vitro and in vivo effects of an anti-CD45 monoclonal antibody in the rat

Cited by 3 publications

References 4 publications

Expression of cytokines in acute heart transplantation rejection

Expression of cytokines in acute heart transplantation rejection

Features of tolerance achieved by antigen and a single injection of an anti-CD45 monoclonal antibody in rats

The Biology of CD45 and its Use as a Therapeutic Target

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