In Vitro and In Vivo Evaluations of Nano-Hydroxyapatite/Polyamide 66/Glass Fibre (n-HA/PA66/GF) as a Novel Bioactive Bone Screw

Su, Bao; Peng, Xiaohua; Jiang, Dianming; Wu, Jun; Qiao, Bo; Li, Weichao; Qi, Xiaotong

doi:10.1371/journal.pone.0068342

Cited by 27 publications

(38 citation statements)

References 30 publications

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“…In the experimental group, bone screws were implanted in the femoral condyle according to the method reported previously. 19 First, 3% pentobarbital (Sigma-Aldrich) intraperitoneal injections for anesthesia (1 mL/kg) were given to the dogs. The lower limbs were shaved and disinfected.…”

Section: Quantitative Real-time Polymerase Chain Reaction Assaymentioning

confidence: 99%

“…To overcome this difficulty, many recent studies have used toughening materials, such as glass fibers and carbon fibers, to enhance the mechanical properties of nHA/PA66 composites. 17,19,20 Graphene is another typical additive used for toughening of polymer materials. 21 Graphene has a two-dimensional honeycomb crystal structure of tightly packed SP2 hybridized carbon atoms 22 and is known to be the thinnest, lightest, and strongest material.…”

Section: Introductionmentioning

confidence: 99%

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In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

Zhang¹,

Yang²,

Zhao³

et al. 2016

IJN

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Graphene and its derivatives have been receiving increasing attention regarding their application in bone tissue engineering because of their excellent characteristics, such as a vast specific surface area and excellent mechanical properties. In this study, graphene-reinforced nanohydroxyapatite/polyamide66 (nHA/PA66) bone screws were prepared. The results of scanning electron microscopy observation and X-ray diffraction data showed that both graphene and nHA had good dispersion in the PA66 matrix. In addition, the tensile strength and elastic modulus of the composites were significantly improved by 49.14% and 21.2%, respectively. The murine bone marrow mesenchymal stem cell line C3H10T1/2 exhibited better adhesion and proliferation in graphene reinforced nHA/PA66 composite material compared to the nHA/PA66 composites. The cells developed more pseudopods, with greater cell density and a more distinguishable cytoskeletal structure. These results were confirmed by fluorescent staining and cell viability assays. After C3H10T1/2 cells were cultured in osteogenic differentiation medium for 7 and 14 days, the bone differentiation-related gene expression, alkaline phosphatase, and osteocalcin were significantly increased in the cells cocultured with graphene reinforced nHA/PA66. This result demonstrated the bone-inducing characteristics of this composite material, a finding that was further supported by alizarin red staining results. In addition, graphene reinforced nHA/PA66 bone screws were implanted in canine femoral condyles, and postoperative histology revealed no obvious damage to the liver, spleen, kidneys, brain, or other major organs. The bone tissue around the implant grew well and was directly connected to the implant. The soft tissues showed no obvious inflammatory reaction, which demonstrated the good biocompatibility of the screws. These observations indicate that graphene-reinforced nHA/PA66 composites have great potential for application in bone tissue engineering.

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Section: Quantitative Real-time Polymerase Chain Reaction Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

Zhang¹,

Yang²,

Zhao³

et al. 2016

IJN

Self Cite

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“…When used in the fabrication of screws can be loaded with GFs to confer additional biological activities to the material and successfully fix intercondylar femur fractures (38) . In addition, related hydroxyapatite materials can be loaded with antibiotics for sustained release in vivo to prevent bacterial infections subsequent to surgery (39) .…”

Section: Engineering Scaffold Topographymentioning

confidence: 99%

Integrating Mechanical and Biological Control of Cell Proliferation Through Bioinspired Multieffector Materials

Seras‐Franzoso

Tatkiewicz

Vázquez

et al. 2015

Nanomedicine (Lond.)

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In nature, cells respond to complex mechanical and biological stimuli whose understanding is required for tissue construction in regenerative medicine. However, the full replication of such bimodal effector networks is far to be reached. Engineering substrate roughness and architecture allows regulating cell adhesion, positioning, proliferation, differentiation and survival, and the external supply of soluble protein factors (mainly growth factors and hormones) has been long applied to promote growth and differentiation. Further, bio-inspired scaffolds are progressively engineered as reservoirs for the in situ sustained release of soluble protein factors from functional topographies. We review here how research progresses towards the design of integrative, holistic scaffold platforms based on the exploration of individual mechanical and biological effectors and their further combination.

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“…Appropriate biomechanical strength, inability to be absorbed and degraded, ease of processing, lack of toxicity and sterilization resistance made PA the ideal candidate for permanent bone replacement [10,11]. However, PA lacks bioactivity with natural bone and cannot provide Ca and P elements for bone reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Interfacial and biological properties of the gradient coating on polyamide substrate for bone substitute

Huang

Niu

Wei

et al. 2014

J. R. Soc. Interface.

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Fabrication of bioactive and mechanical matched bone substitutes is crucial for clinical application in bone defects repair. In this study, nano-hydroxyapatite/ polyamide (nHA/PA) composite was coated on injection-moulded PA by a chemical corrosion and phase-inversion technique. The shear strength, gradient composition and pore structure of the bioactive coating were characterized. Osteoblast-like MG63 cells were cultured on pure PA and composite-coated PA samples. The cells' adhesion, spread and proliferation were determined using MTT assay and microscopy. The results confirm that the samples with the nHA/PA composite coating have better cytocompatibility and have no negative effects on cells. To investigate the in vivo biocompatibility, both pure PA and composite-coated PA cylinders were implanted in the trochlea of rabbit femurs and studied histologically, and the bonding ability with bone were determined using push-out tests. The results show that composite-coated implants exhibit better biocompatibility and the shear strength of the composite-coated implants with host bone at 12 weeks can reach 3.49 + 0.42 MPa, which is significantly higher than that of pure PA implants. These results indicate that composite-coated PA implants have excellent biocompatibility and bonding abilities with host bone and they have the potential to be applied in repair of bone defects.

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In Vitro and In Vivo Evaluations of Nano-Hydroxyapatite/Polyamide 66/Glass Fibre (n-HA/PA66/GF) as a Novel Bioactive Bone Screw

Cited by 27 publications

References 30 publications

In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

Integrating Mechanical and Biological Control of Cell Proliferation Through Bioinspired Multieffector Materials

Interfacial and biological properties of the gradient coating on polyamide substrate for bone substitute

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