In Vitro and In Vivo Studies of Hydrophilic Electrospun PLA95/β-TCP Membranes for Guided Tissue Regeneration (GTR) Applications

Chen, Chien‐Chung; Lee, Sheng-Yang; Teng, Nai Chia; Hu, Hsin-Tai; Huang, Pei-Chi; Yang, Jen‐Chang

doi:10.3390/nano9040599

Cited by 20 publications

(14 citation statements)

References 35 publications

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“…Substantial research is currently focused on the potential utility of electrospun scaffolds for clinical applications including the repair of diaphragm [1], bladder [2][3][4], and ligaments [5][6][7][8], as well as grafting procedures for bone [5,9], skin [10][11][12], and vascular [13,14] tissue. Electrospun scaffolds provide a 3-dimensional fibrous matrix with interconnecting pores, a feature that mimics the native extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

et al. 2019

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Background: There is substantial interest in electrospun scaffolds as substrates for tissue regeneration and repair due to their fibrous, extracellular matrix-like composition with interconnected porosity, cost-effective production, and scalability. However, a common limitation of these scaffolds is their inherently low mechanical strength and stiffness, restricting their use in some clinical applications. In this study we developed a novel technique for 3D printing a mesh reinforcement on electrospun scaffolds to improve their mechanical properties. Methods: A poly (lactic acid) (PLA) mesh was 3D-printed directly onto electrospun scaffolds composed of a 40:60 ratio of poly(ε-caprolactone) (PCL) to gelatin, respectively. PLA grids were printed onto the electrospun scaffolds with either a 6 mm or 8 mm distance between the struts. Scanning electron microscopy was utilized to determine if the 3D printing process affected the archtitecture of the electrospun scaffold. Tensile testing was used to ascertain mechanical properties (strength, modulus, failure stress, ductility) of both unmodified and reinforced electrospun scaffolds. An in vivo bone graft model was used to assess biocompatibility. Specifically, reinforced scaffolds were used as a membrane cover for bone graft particles implanted into rat calvarial defects, and implant sites were examined histologically. Results: We determined that the tensile strength and elastic modulus were markedly increased, and ductility reduced, by the addition of the PLA meshes to the electrospun scaffolds. Furthermore, the scaffolds maintained their matrix-like structure after being reinforced with the 3D printed PLA. There was no indication at the graft/tissue interface that the reinforced electrospun scaffolds elicited an immune or foreign body response upon implantation into rat cranial defects. Conclusion: 3D-printed mesh reinforcements offer a new tool for enhancing the mechanical strength of electrospun scaffolds while preserving the advantageous extracellular matrix-like architecture. The modification of electrospun scaffolds with 3D-printed reinforcements is expected to expand the range of clinical applications for which electrospun materials may be suitable.

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Section: Introductionmentioning

confidence: 99%

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

et al. 2019

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“…Silk fibroin nanofiber mats were prepared by using an electrospinning apparatus detail reported elsewhere [ 36 ]. The formic acid was utilized to prepare 10 wt% silk fibroin solutions.…”

Section: Methodsmentioning

confidence: 99%

An Insight into Nano Silver Fluoride-Coated Silk Fibroin Bioinspired Membrane Properties for Guided Tissue Regeneration

Pandey

Yang

et al. 2021

Polymers

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The current work focuses on the development of a novel electrospun silk fibroin (SF) nonwoven mat as a GTR membrane with antibacterial, biomineralization and biocompatible properties. The γ-poly glutamic acid (γ-PGA)-capped nano silver fluoride (NSF) and silver diamine fluoride (SDF) were first synthesized, which were dip-coated onto electrospun silk fibroin mats (NSF-SF and SDF-SF). UV-Vis spectroscopy and TEM depicted the formation of silver nanoparticles. NSF-SF and SDF-SF demonstrated antibacterial properties (against Porphyromonas gingivalis) with 3.1 and 6.7 folds higher relative to SF, respectively. Post-mineralization in simulated body fluid, the NSF-SF effectively promoted apatite precipitation (Ca/P ~1.67), while the SDF-SF depicted deposition of silver nanoparticles, assessed by SEM-EDS. According to the FTIR-ATR deconvolution analysis, NSF-SF portrayed ~75% estimated hydroxyapatite crystallinity index (CI), whereas pure SF and SDF-SF demonstrated ~60%. The biocompatibility of NSF-SF was ~82% when compared to the control, while SDF-coated samples revealed in vitro cytotoxicity, further needing in vivo studies for a definite conclusion. Furthermore, the NSF-SF revealed the highest tensile strength of 0.32 N/mm and 1.76% elongation at break. Therefore, it is substantiated that the novel bioactive and antibacterial NSF-SF membranes can serve as a potential candidate, shedding light on further in-depth analysis for GTR applications.

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“…125 These GTR membranes showed comparable clinical results of reduced probing depth and increased attachment gaining for periodontal patients suggesting bone cell response enhancement result from coating b-TCP. 126 Additionally, surface modification of scaffold materials has also been studied. Qian successfully coated scaffold with 2% collagen I solution without changing its original elasticity modulus and fracture stress.…”

Section: Surface Modificationmentioning

confidence: 99%

Biodegradable engineered fiber scaffolds fabricated by electrospinning for periodontal tissue regeneration

Ren

et al. 2020

J Biomater Appl

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Considering the specificity of periodontium and the unique advantages of electrospinning, this technology has been used to fabricate biodegradable tissue engineering materials for functional periodontal regeneration. For better biomedical quality, a continuous technological progress of electrospinning has been performed. Based on property of materials (natural, synthetic or composites) and additive novel methods (drug loading, surface modification, structure adjustment or 3 D technique), various novel membranes and scaffolds that could not only relief inflammation but also influence the biological behaviors of cells have been fabricated to achieve more effective periodontal regeneration. This review provides an overview of the usage of electrospinning materials in treatments of periodontitis, in order to get to know the existing research situation and find treatment breakthroughs of the periodontal diseases.

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In Vitro and In Vivo Studies of Hydrophilic Electrospun PLA95/β-TCP Membranes for Guided Tissue Regeneration (GTR) Applications

Cited by 20 publications

References 35 publications

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds

An Insight into Nano Silver Fluoride-Coated Silk Fibroin Bioinspired Membrane Properties for Guided Tissue Regeneration

Biodegradable engineered fiber scaffolds fabricated by electrospinning for periodontal tissue regeneration

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