In vitro and in vivo evaluation of whey protein hydrogels for oral delivery of riboflavin

O’Neill, Graham J.; Jacquier, Jean-Christophe; Mukhopadhya, Anindya; Egan, Thelma; O’Sullivan, M.; Sweeney, T.; O’Riordan, Dolores

doi:10.1016/j.jff.2015.09.043

Cited by 30 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The release kinetics of RF can be fitted by a pseudo first-order kinetic model very well. In addition, in vitro and in vivo release of RF from microbeads was evaluated [110]. In the first hour, the release of RF from dried microbeads was decreased in comparison to wet microbeads in gastric and intestinal settings, with 58% and 34%, respectively.…”

Section: Rf Encapsulationmentioning

confidence: 99%

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Suwannasom

Kao

Pruß

et al. 2020

IJMS

225

107

View full text Add to dashboard Cite

Riboflavin (RF) is a water-soluble member of the B-vitamin family. Sufficient dietary and supplemental RF intake appears to have a protective effect on various medical conditions such as sepsis, ischemia etc., while it also contributes to the reduction in the risk of some forms of cancer in humans. These biological effects of RF have been widely studied for their anti-oxidant, anti-aging, anti-inflammatory, anti-nociceptive and anti-cancer properties. Moreover, the combination of RF and other compounds or drugs can have a wide variety of effects and protective properties, and diminish the toxic effect of drugs in several treatments. Research has been done in order to review the latest findings about the link between RF and different clinical aberrations. Since further studies have been published in this field, it is appropriate to consider a re-evaluation of the importance of RF in terms of its beneficial properties.

show abstract

Section: Rf Encapsulationmentioning

confidence: 99%

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Suwannasom

Kao

Pruß

et al. 2020

IJMS

225

107

View full text Add to dashboard Cite

show abstract

“…These give rise to the possibility of intermolecular and intramolecular disulfide link interchange throughout conformational changes related to pH modifications, temperature, or stress treatment. 23 As a result, this physicochemical property makes whey protein isolate a suitable biopolymer to fabricate microparticles for the development of drug delivery systems to encapsulate a variety of bioactive compounds, such as polyphenols, 32,33 diacetyl, 34 insulin, 35 vitamin D 3 , 36 saffron, 37 essential oil, 38 riboflavin, 39,40 and probiotic bacteria. [41][42][43] In this work, we suggest a new improved strategy for drug delivery using a combination of alginate with different natural biopolymers.…”

mentioning

confidence: 99%

Development of caffeine‐encapsulated alginate‐based matrix combined with different natural biopolymers, and evaluation of release in simulated mouth conditions

Mohammadi

Ehsani

Bakhoda

2018

Flavour & Fragrance J

View full text Add to dashboard Cite

Caffeine was encapsulated into calcium alginate microcapsules, and these microcapsules were produced with four types of material – starch, xanthan, chitosan, and whey protein isolate – in combination with alginate. Formulation characteristics such as the efficiency of microencapsulation, microcapsule size, viscosity, and texture properties of the microcapsules have been evaluated. The release of caffeine from different microcapsules was conducted in simulated saliva fluid. Alginate microcapsules with chitosan coating provided the slowest release of caffeine, compared with the other microcapsules. Significant differences in the appearance of the microcapsules were detectable by scanning electron microscopy (SEM) and optical microscopy. Fourier‐transform infrared spectrometry (FTIR) analysis confirmed the existence of carbonyl groups in caffeine‐loaded microcapsules, and differential scanning calorimetry (DSC) thermograms indicated the glass transition temperature of the microcapsules. This study showed that the microencapsulation of caffeine with alginate may be helpful for designing fast (alginate) as well as prolonged caffeine release (alginate–starch–xanthan with chitosan).

show abstract

“…Overall, the cell viability decreased as the exposure concentration increased (p ≤ 0.05). According to the literature, β-Lg is a Generally Recognized As Safe (GRAS) bio-based material (Ramos et al, 2017;Tavares, Ramos, & Malcata, 2017) ( and riboflavin is an essential compound for the human body (Madadlou, Floury, Pezennec, & Dupont, 2018;Madalena et al, 2016;O'Neill et al, 2015). Thus, the adverse effect of such structures could be related to their concentration.…”

Section: β-Lg Micro-and Nanostructures Effect On Cell Viabilitymentioning

confidence: 99%

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

Simões

Martins

Pinheiro

et al. 2020

Food Research International

View full text Add to dashboard Cite

Lactoglobulin (β-Lg) is known to be capable to bind hydrophilic and hydrophobic bioactive compounds. This research aimed to assess the in vitro performance of β-Lg micro-(diameter ranging from 200 to 300 nm) and nano (diameter < 100 nm) structures associated to hydrophilic and hydrophobic model compounds on Caco-2 cells and under simulated gastrointestinal (GI) conditions. Riboflavin and quercetin were studied as hydrophilic and hydrophobic model compounds, respectively. Cytotoxicity experiment was conducted using in vitro cellular model based on human colon carcinoma Caco-2 cells. Moreover, the digestion process was simulated using the harmonized INFOGEST in vitro digestion model, where samples were taken at each phase of digestion processoral, gastric and intestinal-and characterized in terms of particle size, polydispersity index (PDI), surface charge by dynamic light scattering (DLS); protein hydrolysis degree by 2,4,6-trinitrobenzene sulfonic acid (TNBSA) assay and native polyacrylamide gel electrophoresis; and bioactive compound concentration. Caco-2 cell viability was not affected up to 21 × 10 −3 mg mL −1 of riboflavin and 16 × 10 −3 mg mL −1 quercetin on β-Lg micro-and nanostructures. In the oral phase, β-Lg structures' particle size, PDI and surface charge values were not changed comparing to the initial β-Lg structures (i.e., before being subjected to in vitro GI digestion). During gastric digestion, β-Lg structures were resistant to proteolytic enzymes and to acid environment of the stomachconfirmed by TNBSA and native gel electrophoresis. In vitro digestion results indicated that β-Lg micro-and nanostructures protected both hydrophilic and hydrophobic compounds from gastric conditions and deliver them to target site (i.e., intestinal phase). In addition, β-Lg structures were capable to enhance riboflavin and quercetin bioaccessibility and bioavailability potential compared to bioactive compounds in their free form. This study indicated that β-Lg micro-and nanostructures were capable to enhance hydrophilic and hydrophobic compounds bioavailability potential and they can be used as oral delivery systems.

show abstract

In vitro and in vivo evaluation of whey protein hydrogels for oral delivery of riboflavin

Cited by 30 publications

References 24 publications

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Development of caffeine‐encapsulated alginate‐based matrix combined with different natural biopolymers, and evaluation of release in simulated mouth conditions

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

Contact Info

Product

Resources

About