In vitro and in vivo assessment of anti-inflammatory and immunomodulatory activities of Xylaria nigripes mycelium

Divate, Rupesh D.; Chung, Yun‐Chin

doi:10.1016/j.jff.2017.05.027

Cited by 22 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Normally, inflammation is a protective action by the organism that initiates the healing process and removes the injurious stimuli. − However, in pathological situations, chronic inflammation can also lead to numerous diseases in the host, such as rheumatoid arthritis, atherosclerosis, glomerulonephritis, gastroenteritis, Alzheimer’s disease, Parkinson’s disease, and others, sometimes even causing death. ,, Recently, with evidence continually being identified, inflammatory cytokines and mediators have been regarded as the main contributors in the development of chronic inflammation and inflammation-associated diseases. Reportedly, excessive secretion of inflammatory mediators (e.g., leukotriene, histamine, and prostaglandin E 2 or PGE 2 ) causes the recruitment and extravasation of leukocytes and the secretion of inflammatory cytokines such as tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β), which then promote the secretion of other inflammatory mediators and recruit macrophages to the site of inflammation, finally making the disease worse .…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory Mechanism Involved in 4-Ethylguaiacol-Mediated Inhibition of LPS-Induced Inflammation in THP-1 Cells

Zhao

Jiang

et al. 2019

J. Agric. Food Chem.

View full text Add to dashboard Cite

4-Ethylguaiacol, a common aroma compound of baijiu (a traditional Chinese alcoholic beverage), was assessed for its potential anti-inflammatory effects in an LPS-induced THP-1 cell model. To characterize the effect of 4-ethylguaiacol on the LPS-induced inflammatory response, the mRNA and protein expression of the TLR4−MAPKs−NF-κB−IκBα−AP-1, Nrf2−HO-1, and AMPK−SIRT1 pathways were monitored by ELISA, real-time PCR, and Western blotting. On the basis of the result, 4-ethylguaiacol exerted anti-inflammatory effects at doses of 10, 100, and 500 μM (the concentration of 4-ethylguaiacol in gujinggong baijiu is in the range of 1044 ± 44 to 1661 ± 63 μg/L) and significantly mitigated LPS-induced inflammation via activation of the Nrf2−HO-1 and AMPK−SIRT1 pathways and inhibition of NF-κB and AP-1 activation, thereby markedly inhibiting the activation of inflammasomes and down-regulating the production of inflammatory cytokines. These results indicated that 4-ethylguaiacol could reverse LPS-induced inflammatory responses and is a natural, potent anti-inflammatory component in baijiu.

show abstract

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory Mechanism Involved in 4-Ethylguaiacol-Mediated Inhibition of LPS-Induced Inflammation in THP-1 Cells

Zhao

Jiang

et al. 2019

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…As known, inflammation is an innate process of the immune system responsible for repairing damaged tissue, healing injury, and protecting the host against infection (Divate & Chung, ). Although accumulating evidence has identified that inflammation is a double‐edged sword.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of The Anti‐Inflammatory Effect of Vanillin In Lps‐Activated THP‐1 Cells

Zhao

Jiang

et al. 2019

Journal of Food Science

View full text Add to dashboard Cite

Vanillin, a kind of phenolic compound, is naturally found in food and beverage and widely used as a flavoring agent. In view of the safety and universality of vanillin, exploring the functions of vanillin on human is of great value. Thus, lipopolysaccharide (LPS)‐activated THP‐1 cells were selected as the cell model to evaluate the anti‐inflammatory effect of vanillin in this study. On the basis of the results, vanillin markedly suppressed the expression of inflammatory cytokines (that is, TNF‐α, IL‐1β, IL‐6, and IL‐8), mediators (NO, iNOS, PGE2, and COX‐2), and NLRP3 inflammasome (that is, NLRP3, ASC, and caspase‐1), blocked the LPS‐induced activation of the NF‐κB/IκBα/AP‐1 signaling pathway, and activated the gene expression of the Nrf2/HO‐1 signaling pathway. In addition, it was confirmed that vanillin was unable to react with LPS due to the results of quantification by HS‐SPME‐GC‐MS. Hence, vanillin could effectively attenuate LPS‐induced inflammatory response by regulating the expression of intracellular signaling pathways in THP‐1 cells. It is a potent anti‐inflammatory component found in food and beverage. These findings might contribute to the overall understanding of the potential health benefits of vanillin for food application. Practical Application In this study, the anti‐inflammatory effect of vanillin (VA) was evaluated by ELISA, real‐time PCR, and western blot in LPS‐induced THP‐1 cells. The hypothesis that VA could react with LPS was excluded due to the results of quantification by HS‐SPME‐GC‐MS. On the basis of the result, vanillin could effectively attenuate LPS‐induced inflammatory response in THP‐1 cells and was a potent anti‐inflammatory component natural in food and beverage. These findings might contribute to the overall understanding of the potential health benefits of vanillin for food application.

show abstract

“…Immunomodulators help stimulate the immune system to increase host defenses against pathogens and many autoimmune diseases. Many antifungal compounds show immunomodulatory activity with complement activation and cytokine tissue; natural killer cell stimulation; effects on the activation, differentiation, and effector of T cell and B cell functions; and depends on the response of the macrophages (Divate and Chung, 2017). Formerly the spleen was considered a neglected organ, but now the spleen is considered to play a central role in immune regulation through T-cell mediated phagocytosis and B cell-mediated humoral immunity (González-Gallego et al, 2018).…”

Section: Immunomodulatory Activity Test In Vivomentioning

confidence: 99%

Immunomodulatory Activity Test of Syrup Dosage Form of Combination Phyllantus niruri Linn. And Sterculia quadrifida R.Br. Extract

Rollando

Engracia

Monica

et al. 2020

ijrps

View full text Add to dashboard Cite

Meniran (Phyllantus niruri Linn.) is used generally as an immunomodulator and hepatoprotector. Empirically, bark of faloak (Sterculiaqudri ida R.Br.) is used as a medicinal plant to cure diseases such as hepatitis, gastroentritis, and diabetes. The combination of meniran extract and bark of faloak extract has the potential as a natural immunomodulatory agents. Immunomodulators are compositions of either recombinant, synthetic, or natural, which can restore the immune system. Therefore it is necessary to optimize the optimal formula in obtaining syrup preparations which have optimal immunomodulatory activity. The aim of this study was to ind out the optimal syrup combination of meniran extract and faloak bark extract to produce optimal response values in the in vitro immunomodulatory activity test with TNF-α and NF-κB parameters, and immunomodulatory activity tests in vivo with activity parameters macrophages. The extract was obtained through re lux extraction. The results of the extract were formulated and approved to determine their ability to induce TNF-α and NF-κB, and in the test of mice balb/b (Mus musculus L.) orally for 21 days. The lymph organs were isolated, rinsed with an RPMI-1640 solution, and analyzed using ELISA reader 450 nm. The determination of optimal formulas is done by using Rstudio v.3.5.3 software with ANOVA test parametric test and using Design Expert v.11 software using the Simplex Lattice Design (SLD) method. The results showed that the formula for the preparation of combination syrup to produce results induced TNF-α and NF-κB, and macrophage activity, namely the formula using meniran and faloak 0.96993: 0.0300704.

show abstract

In vitro and in vivo assessment of anti-inflammatory and immunomodulatory activities of Xylaria nigripes mycelium

Cited by 22 publications

References 32 publications

Anti-inflammatory Mechanism Involved in 4-Ethylguaiacol-Mediated Inhibition of LPS-Induced Inflammation in THP-1 Cells

Anti-inflammatory Mechanism Involved in 4-Ethylguaiacol-Mediated Inhibition of LPS-Induced Inflammation in THP-1 Cells

Elucidation of The Anti‐Inflammatory Effect of Vanillin In Lps‐Activated THP‐1 Cells

Immunomodulatory Activity Test of Syrup Dosage Form of Combination Phyllantus niruri Linn. And Sterculia quadrifida R.Br. Extract

Contact Info

Product

Resources

About