In vitro and in silico analysis reveals antifungal activity and potential targets of curcumin on Paracoccidioides spp.

Proteomic Response of Paracoccidioides brasiliensis Exposed to the Antifungal 4-Methoxynaphthalene-N-acylhydrazone Reveals Alteration in Metabolism

“…It inhibited ribosomal biogenesis in Candida species, Aspergillus fumigatus and Cryptococcus neoformans [ 31 ]. Proteomic approaches showed that camphene-thiosemicarbazide [ 29 ], curcumin [ 22 ] and argentilactone [ 28 ] also reduced the protein synthesis of Paracoccidioides spp.…”

“…The quantification of reactive oxygen species (ROS) was performed by fluorescence microscopy following the protocol established by Rocha et al [ 22 ]. Samples containing 1×10 5 fungal cells/mL were exposed to 0.75 μM (0.48 μg/mL) of AOS or without the compound for 12 h. Then, 1 mL of each condition was collected and centrifuged at 3000× g .…”

“…The quantification of reactive oxygen species (ROS) was performed by fluorescence microscopy following the protocol established by Rocha et al [22]. Samples containing 1×10 5 fungal cells/mL were exposed to 0.75 µM (0.48 µg/mL) of AOS or without the compound for 12 h. Then, 1 mL of each condition was collected and centrifuged at 3000× g. The pellet was resuspended in 1 mL of 1X PBS and 25 µM of dichlorodihydrofluorescein 2 , 7 -diacetate dye (DCFH-DC) (Sigma-Aldrich) was added to the samples, which were incubated in the dark for 30 min.…”

Profiling of Antifungal Activities and In Silico Studies of Natural Polyphenols from Some Plants

“…Elagic acid and caffeic acid are recently reported to inhibit Candida auris by modifying fungal cell wall [ 15 ]. Another polyphenol, curcumin has shown a synergistic effect with itraconazole, co-trimoxazole, and amphotericin B by enhancing the reactive oxygen species production [ 16 ]. Nowadays, in silico tools are being used as an efficient method to predict the anti-microbial efficacy of phytobiotics [ 17 ].…”

“…In this regard, target identification for specific polyphenol should be focused for developing plant based drugs. For instance, superoxide dismutase, catalase, and isocitrate lyase have been proposed as molecular targets for curcumin via in silico studies [ 16 ]. Likewise, other plant polyphenols should be assessed for targeting specific fungal enzymes.…”