2008
DOI: 10.1021/tx800244u
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In Vitro and in Silico Characterization of Peroxiredoxin 6 Modified by 4-Hydroxynonenal and 4-Oxononenal

Abstract: Peroxiredoxin 6 (PRX6) belongs to the 1-Cys class of peroxiredoxins and is recognized as an important antioxidant protein in tissues such as cardiac muscle, skin, and lung. Preliminary in vivo proteomic data have revealed that PRX6 is adducted by 4-hydroxynonenal (4HNE) in the livers of rats chronically fed an ethanol-containing diet. The goals of this study were to evaluate the in vitro effect of aldehyde adduction on PRX6 peroxidase activity, identify specific sites of aldehyde modification using mass spectr… Show more

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Cited by 47 publications
(70 citation statements)
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“…The HNE-modified protein spots could be clearly visualized after immunoblotting. The number of spots that was obtained indicated that a large number of proteins can be modified by HNE in vitro, which confirms previous data by other authors (Roede et al 2008).…”
Section: Discussionsupporting
confidence: 90%
See 1 more Smart Citation
“…The HNE-modified protein spots could be clearly visualized after immunoblotting. The number of spots that was obtained indicated that a large number of proteins can be modified by HNE in vitro, which confirms previous data by other authors (Roede et al 2008).…”
Section: Discussionsupporting
confidence: 90%
“…Immunoreactive extra bands migrating close to 35 and 68 kDa may represent the formation of dimers and tetramers, respectively, during the HNE-protein reaction in these experimental conditions. This generation of multimeric species is likely due to the formation of crosslinks between proteins because of the double reactivity of HNE, which can also undergo Michael additions with Cys, His, or Lys residues of one protein molecule and Schiff bases with the Lys residue of another (Roede et al 2008). The absence of trimeric forms suggests that specific spatial conformations may be required for aggregation.…”
Section: Comparison Of the Three Methods For The Detection Of Apo Modmentioning
confidence: 99%
“…Together these reactions can lead to intra- and/or inter-molecular cross-linking of proteins via Michael addition at one site and subsequent Schiff base formation with a neighboring Lys residue [10-12]. Covalent modification by 4-HNE almost invariably leads to an impairment of protein function [2, 12-18] and 4-HNE-mediated adduction/inactivation of specific cellular proteins can have dramatic effects on cell function [19-21]. …”
Section: Introductionmentioning
confidence: 99%
“…Using rodent models of chronic ethanol consumption, our laboratory has demonstrated previously that Prx6 is both down-regulated by chronic ethanol consumption and modified by 4-hydroxynonenal (Roede et al, 2008a). Using Prx6 knockout (KO) mice and transgenic (TG) Prx6 overexpressing mice, we sought to evaluate any mechanistic consequences that chronic ethanol consumption would have on these mice.…”
mentioning
confidence: 99%