In vitro and in silico inhibitory effects of synthetic and natural eugenol derivatives against the NorA efflux pump in Staphylococcus aureus

Muniz, Débora Feitosa; Barbosa, Cristina Rodrigues dos Santos; Menezes, Irwin Rose Alencar de; Sousa, Erlânio Oliveira de; Pereira, Raimundo Luiz Silva; Júnior, João Tavares Calixto; Pereira, Pedro Silvino; Matos, Yedda Maria Lobo Soares de; Costa, Roger Henrique Sousa da; Oliveira-Tintino, Cícera Datiane de Morais; Coutinho, Henrique Douglas Melo; Barbosa-Filho, José Maria; Sousa, Gabriela Ribeiro de; Ribeiro-Filho, Jaime; Siqueira-Júnior, José P.; Tintino, Saulo Relison

doi:10.1016/j.foodchem.2020.127776

Cited by 40 publications

(29 citation statements)

References 37 publications

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“…In a different study, involving molecular docking and in vitro assays, to analyze inhibition of the efflux pump of resistant strains of S. aureus SA1119B and K2068 showing resistance to norfloxacin and ciprofloxacin, the essential oil of Ocotea odorífera and safrole presented promising antibacterial and antibiotic‐enhancing properties by inhibiting the efflux pumps 36 . The compounds, 4‐allyl‐2,6‐dimethoxyphenol, eugenol, and isoeugenol, had marked significant reduction in the MICs of norfloxacin against the SA 1199B strain 37 …”

Section: Discussionmentioning

confidence: 99%

Antibacterial potential of selected phytomolecules: An experimental study

Jhanji

Singh

Kumar

2021

Microbiology and Immunology

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Antibiotic resistance is a snowballing international threat. Some of the antibiotics have lost their effectiveness due to overuse and underuse. Thus, there is an urgent need to tackle this global challenge, either by inhibiting the resistance mechanisms or by the development of new chemical entities. Thus, in the current study, the antibacterial activity of selected phytomolecules was investigated against bacterial strains, alone and in combination, with standard drugs. The antibacterial potential of these phytomolecules was explored using in vitro assays (microtiter assay, bacterial growth kinetics, percentage retardation of growth, and antimicrobial synergy study) and in vivo studies (zebrafish infection model). In vitro and in vivo studies have shown promising antibacterial effects against, both, Gram‐positive and Gram‐negative bacteria. Moreover, a cell viability assay also indicated the cytoprotective effect of these phytomolecules in combination with standard antibiotics (SABX). Thus, these phytomolecules could be a promising broad‐spectrum antibacterial agent in combination with standard antibiotics.

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Section: Discussionmentioning

confidence: 99%

Antibacterial potential of selected phytomolecules: An experimental study

Jhanji

Singh

Kumar

2021

Microbiology and Immunology

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“…Eugenol is an important antimicrobial terpene which can be found in the EOs of plants such as cinnamon or cloves. The study by Muniz et al (2021) reported the antibacterial activity of eugenol and its derivatives including isoeugenol, allylbenzene, 4-allylanisole, and 4-allyl-2,6-dimethoxyphenol against the S. aureus NorA EPs.…”

Section: Phytochemicals For Combating Antibiotic Resistancementioning

confidence: 99%

Exploring Phytochemicals for Combating Antibiotic Resistance in Microbial Pathogens

et al. 2021

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Antibiotic resistance or microbial drug resistance is emerging as a serious threat to human healthcare globally, and the multidrug-resistant (MDR) strains are imposing major hurdles to the progression of drug discovery programs. Newer antibiotic-resistance mechanisms in microbes contribute to the inefficacy of the existing drugs along with the prolonged illness and escalating expenditures. The injudicious usage of the conventional and commonly available antibiotics in human health, hygiene, veterinary and agricultural practices is proving to be a major driver for evolution, persistence and spread of antibiotic-resistance at a frightening rate. The drying pipeline of new and potent antibiotics is adding to the severity. Therefore, novel and effective new drugs and innovative therapies to treat MDR infections are urgently needed. Apart from the different natural and synthetic drugs being tested, plant secondary metabolites or phytochemicals are proving efficient in combating the drug-resistant strains. Various phytochemicals from classes including alkaloids, phenols, coumarins, terpenes have been successfully demonstrated their inhibitory potential against the drug-resistant pathogens. Several phytochemicals have proved effective against the molecular determinants responsible for attaining the drug resistance in pathogens like membrane proteins, biofilms, efflux pumps and bacterial cell communications. However, translational success rate needs to be improved, but the trends are encouraging. This review highlights current knowledge and developments associated challenges and future prospects for the successful application of phytochemicals in combating antibiotic resistance and the resistant microbial pathogens.

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“…Multidrug efflux pumps are cell membrane glycoproteins that can eject different classes of compounds across bacterial membranes; for this reason, they are one of the most important mechanisms of drug resistance, since antibiotics are not capable of reaching the concentration at which they are active within the bacteria [77,78]. Some natural products, including plant extracts, essential oils or isolated compounds, are capable of inhibiting or modifying the efflux pumps' activity [78]. In this context, manuka honey has been proven to induce alterations in the expression of genes belonging to the evgAS regulon, related to bacterial adaptive responses to acid, osmotic and drug resistance [74].…”

Section: Effect On Efflux Pumps Activitymentioning

confidence: 99%

Honey: Another Alternative in the Fight against Antibiotic-Resistant Bacteria?

et al. 2020

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Antibacterial resistance has become a challenging situation worldwide. The increasing emergence of multidrug-resistant pathogens stresses the need for developing alternative or complementary antimicrobial strategies, which has led the scientific community to study substances, formulas or active ingredients used before the antibiotic era. Honey has been traditionally used not only as a food, but also with therapeutic purposes, especially for the topical treatment of chronic-infected wounds. The intrinsic characteristics and the complex composition of honey, in which different substances with antimicrobial properties are included, make it an antimicrobial agent with multiple and different target sites in the fight against bacteria. This, together with the difficulty to develop honey-resistance, indicates that it could become an effective alternative in the treatment of antibiotic-resistant bacteria, against which honey has already shown to be effective. Despite all of these assets, honey possesses some limitations, and has to fulfill a number of requirements in order to be used for medical purposes.

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In vitro and in silico inhibitory effects of synthetic and natural eugenol derivatives against the NorA efflux pump in Staphylococcus aureus

Cited by 40 publications

References 37 publications

Antibacterial potential of selected phytomolecules: An experimental study

Antibacterial potential of selected phytomolecules: An experimental study

Exploring Phytochemicals for Combating Antibiotic Resistance in Microbial Pathogens

Honey: Another Alternative in the Fight against Antibiotic-Resistant Bacteria?

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