In Vitro Analysis of Xenoestrogens by Enzyme Linked Receptor Assays (ELRA)

Seifert, Martin; Haindl, Stefanie; Hock, Bertold

doi:10.1007/978-1-4899-0089-0_13

Cited by 39 publications

(26 citation statements)

References 5 publications

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“…The endocrine effects in the sediments of the Berlin Water Ways the River Havel, Spree, Dahme and Teltowkanal are calculated and expressed as 17ß-estradiol equivalents in [μg/kg] sediment dry weight for pore water and elutriates of sediments in [μg/l] using the ELRA-test (Seifert et al, 1998), see Figures 2 and 3. For the lower River Havel a median concentration was determined of 8.5 μg/kg-17ß-estradiol equivalents, for the upper River Havel of 6.7 μg/kg-17ß-estradiol equivalents, for the River Spree of 34.2 μg/kg-17ß-estradiol equivalents, for the River Dahme of 45.7 μg/kg-17ß-estradiol equivalents and for the Teltowkanal of 14.3 μg/kg-17ß-estradiol equivalents.…”

Section: Results Of Biochemicals Effects In the River System Of Berlinmentioning

confidence: 99%

“…The used enzyme linked receptor assay (ELRA)-Assay is working after the same principle like the competitive immunoassay ELISA based on the ligand-protein interaction and can be used for routine screening of estrogens and xenoestrogens in environmental samples (Seifert et al, 1998). Due to the subcellular level of ELRA, the toxicity and the microbial contamination of samples have no negative effects concerning the test procedure.…”

Section: Description Of the Used Endocrine Effects Assay Testmentioning

confidence: 99%

“…The extinction of enzyme reaction is proportional to the counts of the receptor-17ß-estradiol complexes. At high EDC concentrations in the sample there are lower amounts of receptor-17ß-estradiol complexes and the high receptor-ligand complex which was eliminated by washing previously see Figure 1 ( Seifert et al, 1998).…”

Section: Principle Of the Testmentioning

confidence: 99%

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Ecotoxicological Classification of the Berlin River System Using Bioassays in Respect to the European Water Framework Directive

Huschek

Hansen

2006

Environ Monit Assess

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Bioassays as well as biochemical responses (biomarkers) in ecosystems due to environmental stress provide us with signals (environmentally signalling) of potential damage in the environment. If these responses are perceived in this early stage in ecosystems, the eventual damage can be prevented. Once ecosystem damage has occurred, the remedial action processes for recovery could be expensive and pose certain logistical problems. Ideally, "early warning signals" in ecosystems using sensing systems of biochemical responses (biomarkers) would not only tell us the initial levels of damage, but these signals will also provide us with answers by the development of control strategies and precautionary measures in respect to the European Water Framework Directive (WFD). Clear technical guidelines or technical specifications on monitoring are necessary to establish and characterise reference conditions for use in an ecological status classification system for surface water bodies. For the Ecotoxicological Risk Assessment (ERA) of endocrine effects we used an approach of the exposure - dose - response concept. Based on the "Ecototoxicological Classification System of Sediments" that uses pT-values to classify effects in different river systems, we transferred the bio-monitoring data to the five-level ecological system of the WFD. To understand the complexity of the structure of populations and processes behind the health of populations, communities and ecosystems an ERA should establish links between natural factors, chemicals, and biological responses so as to assess causality. So, our ecological monitoring assessment has incorporated exposure & effects data.

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Section: Results Of Biochemicals Effects In the River System Of Berlinmentioning

confidence: 99%

Section: Description Of the Used Endocrine Effects Assay Testmentioning

confidence: 99%

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Ecotoxicological Classification of the Berlin River System Using Bioassays in Respect to the European Water Framework Directive

Huschek

Hansen

2006

Environ Monit Assess

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“…For this high throughput prescreening (HTPS), α-, and β-estrogens, and androgen receptors are typically used in both binding and reporter gene assays. 30,31 The receptor binding assay for estrogens and androgens is based on the competitive binding of these hormones to estrogen and androgen receptors, respectively. The reporter gene assay for steroid hormones is a serial or double binding assay, in which a steroid-hormone-bound steroid receptor is further bound to a promoter region of a gene to form a functional transcription factor that switches on a reporter gene.…”

Section: Probing Cellular Signaling Pathways In Living Cellsmentioning

confidence: 99%

Methods of Analysis for Chemicals that Promote/Disrupt Cellular Signaling

2002

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“…This approach is still restricted by our understanding of the underlying mechanism of action of such agents, and again, the application to cancer usually involves the assumption of a genotoxic mechanism, although methods to detect possible agents acting through an epigenetic hormonal mechanism have also been developed (Seifert et al, 1998).…”

Section: Carcinogenic Exposurementioning

confidence: 99%

Elevated Levels of Somatic Mutation as a Biomarker of Environmental Effects Contributing to Breast Carcinogenesis

Grant¹

2002

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and AUTHOR(S)Stephen G. Grant, Ph.D. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)8 ABSTRACT (Maximum 200 Words)Risk factors for the development of breast cancer remain largely unknown, especially those associated with the effect of the environment. Despite the known influence of hormonal factors, breast tumorigenesis has been shown to involve an accumulation of serial genetic changes in cancer genes. X-irradiation is a genotoxic exposure that has been identified as a risk factor for breast cancer. If somatic mutation is a major mechanism that drives the process of breast carcinogenesis, we hypothesize that breast cancer should develop preferentially in women with higher frequencies of somatic variation, as measured at a neutral reporter locus. The rate of somatic mutation is determined by exposure to endogenous and exogenous genotoxic agents and by an individual's ability to deal with such exposures through biotransformation of xenobiotics and DNA repair. We are using two established bioassays of in vivo human somatic mutation to study a large population of newly diagnosed primary breast cancer patients. These genetic/environmental interactions can be quantitated simply from observed effect, before we have completely understood the underlying mechanisms. Our primary goal is to provide a rationale for simple laboratory blood tests to be incorporated into the risk estimation equation for breast cancer.14. 3 IntroductionBreast cancer is one of the most common malignancies in the western world, and increases in incidence are being seen worldwide. Risk factors for the development of breast cancer remain largely unknown, with up to 75% of breast cancers occurring in the absence of established risk factors. The three risk factors that have been established are: family history of breast cancer, metabolic factors related to hormone production, and exposure to X-irradiation (Gail and Benichou, 1992;Claus et al., 1994). This study proposes that the last mentioned factor, exposure to ionizing radiation, is actually indicative of a larger involvement of genotoxicity in the etiology of breast cancer. This suggests that exposure to carcinogenic chemicals and susceptibility to mutagenesis and carcinogenesis play a significant role in determining who will develop a tumor of the breast. Indeed, evidence is mounting that the two known breast cancer susceptibility genes, BRCA1 and BRCA2 are actually DNA repair genes that modulate the genotoxi...

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In Vitro Analysis of Xenoestrogens by Enzyme Linked Receptor Assays (ELRA)

Cited by 39 publications

References 5 publications

Ecotoxicological Classification of the Berlin River System Using Bioassays in Respect to the European Water Framework Directive

Ecotoxicological Classification of the Berlin River System Using Bioassays in Respect to the European Water Framework Directive

Methods of Analysis for Chemicals that Promote/Disrupt Cellular Signaling

Elevated Levels of Somatic Mutation as a Biomarker of Environmental Effects Contributing to Breast Carcinogenesis

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