In vitro Analysis of the Na&lt;sup&gt;+&lt;/sup&gt;-K&lt;sup&gt;+&lt;/sup&gt; ATPase Activity in Neonatal and Adult Red Blood Cells

Angelopoulous, Margarite; Leitz, Helen; Lambert, George H.; MacGilvray, Scott

doi:10.1159/000244289

Cited by 17 publications

(5 citation statements)

References 14 publications

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“…These results are in agreement with those of Angelopoulous et al [22] showing a significant diminution in the ouabain-sensitive net K + influx in the neonatal erythrocytes in comparison with the adult erythrocytes. Preliminary experiments carried out with these preparations have shown that the main biochemical characteristics of the Na,K-ATPase (e.g., K m for Na + , K + , and Mg:ATP, optimal pH and temperature, IC 50 for ouabain) are not affected by preeclampsia (data not shown).…”

Section: Discussionsupporting

confidence: 94%

Preeclampsia and Na,K-ATPase Activity of Red Blood Cell Ghosts from Neonatal and Maternal Blood

Carreiras

Proverbio²,

Proverbio³

et al. 2001

Neonatology

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We determined Na,K-ATPase activity and cholesterol/phospholipid ratio of maternal and cord red blood cell ghosts from either normotensive or preeclamptic pregnant women. The Na,K-ATPase activity of the red cell ghosts from neonatal blood is significantly lower (25–32%) as compared with the ATPase activity of the maternal red cell ghosts, regardless of the presence or not of preeclampsia. This diminution in Na,K-ATPase activity of the neonatal red blood cell ghosts could be due to an increase in the cholesterol/phospholipid molar ratio of the membrane. The Na,K-ATPase activity of the red blood cell ghosts from pregnant women was unaffected by preeclampsia; however, fetal red blood cell ghosts from infants of preeclamptic mothers showed a significantly lower ATPase activity (20%) than fetal red blood cell ghosts from infants of normotensive mothers. A low Na,K-ATPase activity in the neonatal red blood cells from mothers with preeclampsia could be an indication of an important modification of the physiological role of this enzyme.

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Section: Discussionsupporting

confidence: 94%

Preeclampsia and Na,K-ATPase Activity of Red Blood Cell Ghosts from Neonatal and Maternal Blood

Carreiras

Proverbio²,

Proverbio³

et al. 2001

Neonatology

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“…The observed salbutamol-induced decrease in serum K + is the result of stimulation of the membrane bound Na + /K + -ATPase by a mechanism that involves the ß 2 -adrenoreceptor [54,69]. The effect of salbutamol on transmembrane potassium flux mediated by stimulation of the Na + /K + -ATPase has been studied in neonatal and adult red blood cells under hyperkalaemic conditions [5]. Salbutamol caused no increase in net transmembrane potassium flux in adult red blood cells, but was associated with a 50% increase of potassium flux in neonatal red blood cells.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Pathogenesis and therapy of non-oliguric hyperkalaemia of the premature infant

Mildenberger

Versmold

2002

Eur J Pediatr

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Non-oliguric hyperkalaemia is a common and serious complication of extreme prematurity, resulting from a potassium loss from the intra- into the extracellular space during a specific post-natal period. Treatment of this disorder has been adapted from the treatment of hyperkalaemia in renal failure, an entity of completely different pathophysiology. A few years ago, the administration of salbutamol, which induces cellular potassium uptake, was proposed as a new therapeutic option. In this review article we discuss the pathogenesis and current therapy of non-oliguric hyperkalaemia of the premature infant, with special emphasis on the presently available knowledge and concerns with regard to the use of salbutamol. Being aware of the paucity of studies on non-oliguric hyperkalaemia, we propose treatment recommendations which are based on best available evidence. These comprise the administration of calcium, infusion of insulin plus glucose, correction of acidosis, and exchange transfusion or peritoneal dialysis as a last resort therapy. Before controlled trials on efficacy of salbutamol treatment of non-oliguric hyperkalaemia of the premature infant can be initiated, more data on safety are needed.

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“…The e ect of salbutamol on transmembrane potassium flux has been studied in neonatal red blood cells under hyperkalaemic conditions and resulted in a 50% increase in net transmembrane potassium flux (Angelopoulous 1996). Salbutamol infusion (but not inhalation) for hyperkalaemia has been reported in neonates (Greenough 1992;Dilmen 1992;Avenarius 1996).…”

Section: Description Of the Interventionmentioning

confidence: 99%