In Vitro Analysis of the Pea Chloroplast 16S rRNA Gene Promoter

Sun, Eric I.; Wu, B. W.; Tewari, Κ. Κ.

doi:10.1128/mcb.9.12.5650-5659.1989

Cited by 1 publication

(4 citation statements)

References 34 publications

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“…Interestingly, clpP is transcribed from the PclpP –53 NEP promoter in wild‐type leaves while most other NEP promoters are inactive in the same tissue. Differential accumulation of mRNAs from NEP promoters is possibly due to regulation by nuclear‐encoded factors via gene‐specific promoter elements, as reported for σ 70 ‐type PEP promoters (Sun et al ., 1989; Iratni et al ., 1994; Allison and Maliga, 1995; Kim and Mullet, 1995).…”

Section: Discussionmentioning

confidence: 55%

“…Group II includes plastid genes encoding mRNAs that factors interacting with elements upstream of the core promoter (Sun et al, 1989;Iratni et al, 1994; Allison accumulate to significant levels in both wild-type and ΔrpoB leaves (Figure 1B). Group II includes atpB (ATP and Maliga, 1995;Kim and Mullet, 1995).…”

Section: Introduction Resultsmentioning

confidence: 99%

“…specific promoter elements, as reported for σ 70 -type PEP Total leaf RNA was prepared from fully-expanded leaves using TRIzol promoters (Sun et al, 1989;Iratni et al, 1994;Allison (Gibco-BRL), following the manufacturer's protocol. The RNA was and Maliga, 1995;Kim and Mullet, 1995).…”

Section: Rna Gel Blotsmentioning

confidence: 99%

“…The promoters utilized by PEP are similar to E.coli σ 70 promoters, consisting of −35 and −10 consensus elements (reviewed in Igloi and Kössel, 1992; Gruissem and Tonkyn, 1993; Link, 1994, 1996). Transcription activity from some PEP promoters is modulated by nuclear‐encoded transcription factors interacting with elements upstream of the core promoter (Sun et al ., 1989; Iratni et al ., 1994; Allison and Maliga, 1995; Kim and Mullet, 1995).…”

Section: Introductionmentioning

confidence: 99%

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The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct groups of genes in tobacco plastids

Hajdukiewicz

Allison

Maliga

1997

EMBO J

471

438

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deleting the rpoB gene encoding the essential β subunit 1 Present address: University of Nebraska, Lincoln, of the tobacco PEP, we established the existence of a N209 Beadle Center, Lincoln, NE 68588-0664, USA second nuclear-encoded plastid RNA polymerase (NEP) in photosynthetic higher plants (Allison et al., 1996). Corresponding authorDeletion of rpoB yielded photosynthetically defective, pigment-deficient plants. An examination of ΔrpoB plants The plastid genome in photosynthetic higher plants encodes subunits of an Escherichia coli-like RNA polyrevealed proplastid-like structures containing low levels of mRNAs for the photosynthetic genes rbcL, psbA and merase (PEP) which initiates transcription from E.coli σ 70 -type promoters. We have previously established psbD due to the lack of PEP promoter activity. In wildtype tobacco leaves, the ribosomal RNA operon (rrn) is the existence of a second nuclear-encoded plastid RNA polymerase (NEP) in photosynthetic higher plants. We transcribed by PEP. Interestingly, in the ΔrpoB plants the rrn mRNA accumulated close to wild-type levels due to report here that many plastid genes and operons have at least one promoter each for PEP and NEP (Class II transcription by NEP acting at a downstream non-σ 70 -type promoter. The rRNA operon is the first plastid transcription unit). However, a subset of plastid genes, including photosystem I and II genes, are transcribed transcription unit for which a promoter for both PEP and NEP was identified. from PEP promoters only (Class I genes), while in some instances (e.g. accD) genes are transcribed excluWe report here that the rRNA operon is not unique, but represents a class of plastid transcription units which have sively by NEP (Class III genes). Sequence alignment identified a 10 nucleotide NEP promoter consensus at least one promoter each for PEP and NEP. These genes or operons have a potential for expression by either of around the transcription initiation site. Distinct NEP and PEP promoters reported here provide a general the two plastid RNA polymerases. Furthermore, some genes are transcribed by only one of the two RNA mechanism for group-specific gene expression through recognition by the two RNA polymerases.polymerases. We propose that transcription by NEP and PEP, through recognition of distinct promoters, is a general Keywords: Nicotiana tabacum/nuclear-encoded plastid RNA polymerase (NEP)/plastid-encoded RNA mechanism of group-specific gene regulation during chloroplast development. A tentative NEP promoter conpolymerase (PEP)/plastid gene expression/rpoB deficient mutant sensus is derived by the alignment of the transcription initiation sites.

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