In vitro analysis of the self-cleaving satellite RNA of barley yellow dwarf virus

Replication of barley yellow dwarf luteovirus-PAV RNA

“…Although initial attempts of obtaining transgenic resistance to luteoviruses have not been very successful, efforts should continue to test new anti-viral strategies. Rjbozyme-175 (see appendix I), which has been shown to cleave the BYDV-PAV RNA in vitro (Silver, 1993), can be introduced into stably transformed oat plants to test its activity in plants. In addition, new anti-BYDV ribozymes should be designed and tested because of the great potential of ribozymes in suppressing replication of RNA in plants (Yang et al, 1997).…”

“…Wegener et al (1994) Anti-BYDV ribozymes showed that the satellite RNA of BYDV contains a novel hammerhead structure in the self-cleavage domain. Later, several ribozymes were designed in our laboratory (Silver, 1993) to cleave the genomic RNA of BYDV-PA.V. Of the three ribozymes that were tested, ribozyme-175 (Rz-175) was shown to cleave most efficiently in vitro (Silver, 1993).…”

“…Later, several ribozymes were designed in our laboratory (Silver, 1993) to cleave the genomic RNA of BYDV-PA.V. Of the three ribozymes that were tested, ribozyme-175 (Rz-175) was shown to cleave most efficiently in vitro (Silver, 1993). Rz-175 was targeted to the 5'-end of ORFl of BYDV-PAV because cleavage ui the 5'-end of the virus may inhibit an early step in virus replication.…”