In vitro analysis of the influence of mineralized and EDTA-demineralized allogenous bone on the viability and differentiation of osteoblasts and dental pulp stem cells

Bertassoli, Bruno Machado; Silva, Gerluza Aparecida Borges; Albergaria, Juliano Douglas Silva; Jorge, Érika Cristina

doi:10.1007/s10561-020-09834-3

Cited by 5 publications

(5 citation statements)

References 50 publications

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Section: Resultssupporting

confidence: 87%

“…S1 †) exposed the dense fibrous nature of the matrix showing no residual calcium (Ca) and phosphate (P) elements. The presence of carbon (C) and oxygen (O) was observed similar to the study by Bertassoli et al 44 Rheological characterization of the BdECM at different concentrations (1% and 3%) helped to understand the physical properties of the hydrogel. Viscosity versus shear rate profile with a negative slope showed the shear-thinning behavior of the gel (Fig.…”

Section: Biomaterials Science Papersupporting

confidence: 77%

See 1 more Smart Citation

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Sukanya

Giri

Nellore³

et al. 2023

Biomater. Sci.

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Section: Resultssupporting

confidence: 87%

Section: Biomaterials Science Papersupporting

confidence: 77%

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Sukanya

Giri

Nellore³

et al. 2023

Biomater. Sci.

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“…13 Demineralization of bone tissues is typically achieved using the EDTA or HCl extraction method. 8,14,32,33,[39][40][41] The treatment with 0.5-0.6 N HCl has been recommended as the most effective method for preserving the osteoinductivity of DBM. 39 Although several studies have reported that the demineralization with EDTA has detrimental effects on the osteoinductivity of DBM, some researchers argued that no difference in bone induction between dentin demineralized using HCl and EDTA.…”

Section: Discussionmentioning

confidence: 99%

“…Demineralization of bone tissues is typically achieved using the EDTA or HCl extraction method 8,14,32,33,39–41 . The treatment with 0.5–0.6 N HCl has been recommended as the most effective method for preserving the osteoinductivity of DBM 39 .…”

Section: Discussionmentioning

confidence: 99%

Enhanced osteogenicity of the demineralized bone‐dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules

Ma,

Ren,

Wang

et al. 2023

J Biomed Mater Res

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Allogenic demineralized bone matrix (DBM), processed to expose bioactive proteins imbedded by calcium salts, is widely used for bone repair and regeneration as an alternative to the autologous bone graft. However, demineralized bone matrices from tissue banks vary significantly in residual calcium content and osteogenicity for clinical bone regeneration. The present study produced DBM with various residual calcium contents by partial demineralization using ethylenediaminetetraacetic acid disodium (EDTA) and hydrochloric acid. Compositional analysis reveals that, as the percent weight loss of bone materials increases from 0% to 74.9% during demineralization, the residual calcium content of DBM decreases from 24.8% to 0.2% and collagen content increases from 29.7% to 92.6%. Calorimetrical analysis and Fourier transform infrared (FTIR) analysis demonstrated that demineralization to the residual calcium content of <4% enables the complete exposure and/or release of bone collagen fibers and other bioactive molecules. In order to evaluate the relationship between the extent of demineralization and the osteogenicity of DBM, DBM particles were fabricated with the aid of acellular dermal matrix (ADM) microfibers to form flexible foam‐like DBM/ADM composites. Proteomic analysis identified various type collagens and bone formation‐related bioactive molecules in both ADM and DBM. Using the rat bilateral Φ = 5 mm calvarium defect repair model, the study had shown that the DBM/ADM composite with ~20% DBM residual calcium (e.g., ~40% calcium being removed) maximized the osteogenicity for bone defect repair after 4 and 8 weeks. DBM with ~40% calcium removal had the maximal osteogenicity presumably through the sustained release of bioactive molecules during the process of bone regeneration.

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“…For example, evidence has demonstrated that DPSCs may be capable of proliferation and wound healing, as well as differentiation into osteogenic and chondrogenic lineages [ 2 , 3 ]. Some research has demonstrated DPSCs may have specific capabilities to facilitate differentiation into osteoblasts [ 4 , 5 ]. However, recent studies have suggested that DPSCs may also harbor the potential for differentiation into other lineages, separate and apart from the odontogenic and osteogenic potential for which they are well known [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Lee

Bae

Kim

et al. 2023

JFB

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) with the potential to differentiate in a limited number of other tissue types. Some evidence has suggested the modulation of DPSC growth may be mediated, in part, by exogenous extracellular matrix (ECM) glycoproteins, including fibronectin (FN) and laminin-5 (LN5). Although preliminary research suggests that some ECM glycoproteins may work as functional biomaterials to modulate DPSC growth responses, the primary goal of this project is to determine the specific effects of FN and LN5 on DPSC growth and viability. Using an existing DPSC repository, n = 16 DPSC isolates were cultured and 96-well growth assays were performed, which revealed FN, LN5 and the combination of these were sufficient to induce statistically significant changes in growth among five (n = 5) DPSC isolates. In addition, the administration of FN (either alone or in combination) was sufficient to induce the expression of alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), while LN5 induced the expression of ALP only, suggesting differential responsiveness among DPSCs. Moreover, these responses appeared to correlate with the expression of MSC biomarkers NANOG, Oct4 and Sox2. These results add to the growing body of evidence suggesting that functional biomaterials, such as ECM glycoproteins FN and LN5, are sufficient to induce phenotypic and differentiation-specific effects in a specific subset of DPSC isolates. More research will be needed to determine which biomarkers or additional factors are necessary and sufficient to induce the differentiation and development of DPSCs ex vivo and in vitro for biomedical applications.

show abstract

In vitro analysis of the influence of mineralized and EDTA-demineralized allogenous bone on the viability and differentiation of osteoblasts and dental pulp stem cells

Cited by 5 publications

References 50 publications

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Enhanced osteogenicity of the demineralized bone‐dermal matrix composite by the optimal partial demineralization for sustained release of bioactive molecules

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

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