In Vitro Analysis of Tartrazine Genotoxicity and Cytotoxicity

Haverić, Anja; Inajetović, Damira; Vareškić, Aneta; Hadžić, Maida; Haverić, Sanin

doi:10.31383/ga.vol1iss1pp37-43

Cited by 4 publications

(1 citation statement)

References 14 publications

(15 reference statements)

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“…11,12 The frequency of mononuclear, binuclear, trinuclear, and tetranuclear lymphocytes, apoptosis, and necrosis was analyzed on at least 500 cells per sample. 13…”

Section: Cytokinesis-block Micronucleus Cytome (Cbmn Cyt) Assaymentioning

confidence: 99%

In vitro analysis of thimerosal genotoxicity and cytotoxicity effect

et al. 2022

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Background: Thimerosal is an organomercury compound with high mercury content. Thimerosal is located in the market as an effective bacteriostatic in a series of pharmaceutical products, ophthalmic and nasal products, immunoglobulin preparations, and as a preservative in vaccines. Aims and Objectives: Since it is a compound with living content, this study aimed to examine the genotoxic and cytotoxic potential of thimerosal in human lymphocytes culture. Materials and Methods: We used chromosome aberration analysis and cytokinesis-block micronucleus cytome (CBMN-Cyt) assay to test its genotoxic and cytotoxic potential in human lymphocyte culture. Results: Results showed that the frequency of structural chromosome aberrations and CBMN-cyt assay was significantly increased in treated cultures (1 μg/ml and 0.5 μg/ml) compared to the negative control. Conclusion: Obtained results and statistical analysis show that thimerosal is genotoxic and cytotoxic in human lymphocytes in tested concentrations.

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“…11,12 The frequency of mononuclear, binuclear, trinuclear, and tetranuclear lymphocytes, apoptosis, and necrosis was analyzed on at least 500 cells per sample. 13…”

Section: Cytokinesis-block Micronucleus Cytome (Cbmn Cyt) Assaymentioning

confidence: 99%

In vitro analysis of thimerosal genotoxicity and cytotoxicity effect

et al. 2022

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Safety of tartrazine in the food industry and potential protective factors

Amchova,

Siska,

Ruda-Kucerova

2024

Heliyon

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Evaluation of the genotoxic effect of tartrazine using a metabolic activation system in human lymphocyte culture under cytokinetic block conditions

Nikitina¹,

Konyashkina²,

Ingel³

et al. 2023

Ecological genetics

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The expansion of the spectrum of use of food additives and, in particular, food dyes (FD), increases the risk of increasing human exposure to genotoxicants. Since in real life, not pure substances with proven genetic safety are in contact with a person, but complex mixtures of unknown composition, even minor impurities in which can become an additional source or modifier of genome instability effects. Of particular concern in this aspect are synthetic FD azo and diazo compounds which can be transformed by human intestinal microflora to some forms of genotoxicants. The purpose of the work is to evaluate the genotoxic effects of 02 mg/mL of Tartrazine FD (E102) purchased in a retail network in a micronucleus test on human blood cells cultured under cytokinetic block conditions in parallel in presence and without rat S9 hepatocyte metabolic activation system. Genotoxic effects were found in cultures without metabolic activation at 0.00002560,00064 mg/mL and 0.4 mg/mL of tartrazine, and in the presence of S9 at 0.0000256 mg/mL, 0,000128 mg/mL and 0.16 mg/mL of tartrazine. For the first time, a dose-dependent suppression of mitotic and proliferative activity of lymphocytes induced by the tested tartrazine sample was revealed, as well as a dose-dependent U-shaped curve in the frequency of apoptosis. The data obtained indicate the presence of genotoxic activity of the studied sample. We discuss the necessity to create the system for evaluation the genotoxic safety of FD real mixtures from a retail network.

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In Vitro Analysis of Tartrazine Genotoxicity and Cytotoxicity

Cited by 4 publications

References 14 publications

In vitro analysis of thimerosal genotoxicity and cytotoxicity effect

In vitro analysis of thimerosal genotoxicity and cytotoxicity effect

Safety of tartrazine in the food industry and potential protective factors

Evaluation of the genotoxic effect of tartrazine using a metabolic activation system in human lymphocyte culture under cytokinetic block conditions

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