In vitro analysis of antispasmodic activity of ethanolic stem extracts of Uvaria rufa Blume and Anomianthus dulcis (Dunal) J. Sinclair on excised rat’s ileum

Wiya, Chattida; Saenphet, Kanokporn; Saenphet, Supap; Buncharoen, Wararut

doi:10.1007/s00580-017-2588-y

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…6 Reports also indicated the antibacterial, 7 and antispasmodic properties of ethanol extract of twigs and stem of the plant. 8 Several studies have been reported on their chemical constituents and some biological activities. Flavonoid glycosides such as kaempferol 3-O-ß-galactopyranoside, rutin, astragalin, isoquercitrin-6-acetate and isoquercitrin, isolated from the leaves of U. rufa inhibited the formation of advanced glycation end-products (AGEs) which contribute to the progression of diabetic complications and aging in the bovine serum (BSA) albumin-glucose assay.…”

Section: Introductionmentioning

confidence: 99%

Anti-mitotic Activities of Ethanolic Extract and Glutinol from Uvaria rufa Blume

Alimboyoguen¹,

Castro-Cruz²,

Shen³

et al. 2023

Ind. J. Pharm. Edu. Res.

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Background: This study describes the isolation of major chemical constituent from the bark of Uvaria rufa Blume, commonly known as "allagat or susong kalabaw", an indigenous Philippine medicinal plant that belongs to the family Annonaceae. It is a short climbing shrub that grows in low and medium-altitude forests in the country. Materials and Methods: The bark samples of U. rufa were air-dried for three months without exposure to sunlight. The samples were ground in a blender, then, soaked in CH 2 Cl 2 and in ethanol for three days and then, filtered. The crude extract of U. rufa was chromatographed on a gravity column dry packed with silica gel and was fractionated by silica gel chromatography using increasing proportion of acetone in CH 2 Cl 2 at 10% increment. The purified isolates were subjected to NMR for structure elucidation. Their structures were identified mainly by using 1 H-NMR and by comparing current NMR data with those reported in the literature. Results: The results showed that the air-dried bark dichloromethane extract of the plant afforded glutinol. Glutinol and ethanolic extract exhibited anti-mitotic activity (18.33 % and 18.93 %, respectively), comparable to methotrexate (19.07 %), a well-known anti-cancer drug and even more potent in which they surpassed by 0.74 % and 0.14 %. Conclusion: The dichloromethane extract of the bark of U. rufa afforded glutinol that can be one the main metabolites responsible for the anti-mitotic activity of the plant. It may also contained other substances that might have great potential as novel therapeutic agents for cancer.

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Section: Introductionmentioning

confidence: 99%

Anti-mitotic Activities of Ethanolic Extract and Glutinol from Uvaria rufa Blume

Alimboyoguen¹,

Castro-Cruz²,

Shen³

et al. 2023

Ind. J. Pharm. Edu. Res.

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“…The chemical constituents of the twigs, stems and leaves have been identified as polyoxygenated cyclohexenes (Kaweetripob et al 2015), acetogenins (Sinz et al 1998a) phenylpropanoid amides (Sinz et al 1999), flavonoids (Chantrapromma et al 2001;Ubonopas et al 2014) and alkaloids (Sinz et al 1998b;Ubonopas et al 2014). The crude extract and some secondary metabolites from this plant showed antibacterial (Ubonopas et al 2014;Kadchumsang et al 2015), anticancer, anti-Herpes Simplex Virus I (Ubonopas et al 2014), antispasmodic, (Wiya et al 2018) and antioxidant activities (Kadchumsang et al 2015). Furthermore, previous investigations of plants from the Uvaria genus have found several types of secondary metabolites such as polyoxygenated cyclohexenes (Ho et al 2015;Okpekon et al 2015;Hsu et al 2016;Auranwiwat et al 2017;Awale et al 2017;Auranwiwat et al 2019a;Auranwiwat et al 2019b), flavonoids (Salae et al 2017;Auranwiwat et al 2018), alkaloids (Auranwiwat et al 2018), naphthalenes (Auranwiwat et al 2017;Salae et al 2017), xanthenes (Macabeo et al 2014) and lignan glycosides (Nguyen et al 2015).…”

Section: Introductionmentioning

confidence: 99%

(R)-3-(8'-Hydroxyfarnesyl)-indole and other chemical constituents from the flowers of Anomianthus dulcis and their antimalarial and cytotoxic activities

Promchai

Thaima

Rattanajak

et al. 2019

Natural Product Research

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“…Moreover, brazilin and hematoxylin isolated from the heartwood of C. sappan also exhibited relaxant effects on isolated rats' aorta [22]. The ethanolic extract from the stems of U. rufa are used to remedy gastrointestinal disorder [23]. The fruits of U. rufa are used against skin allergies and gastrointestinal abscesses [24].…”

Section: Introductionmentioning

confidence: 99%

Relaxant Activities of Extracts from Uvaria rufa Blume and Caesalpinia sappan L. on Excised Rat’s Prostate Strips

Buncharoen

Saenphet

2019

JPRI

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Aims: To determine the relaxant activity of various extracts from the stems of Uraria rufa Blume and Caesalpinia sappan L. on rats’ prostate strips in vitro. Study Design: The relaxant efficacies of ethyl acetate and ethanolic extracts from the stems of U. rufa (UEA and UEOH) and C. sappan (CEA and CEOH) were tested on isolated rats’ prostate tissue pre-contracted by adrenaline. Place and Duration of Study: Department of Biology, Faculty of Science, Chiang Mai University, between February and September 2018. Methodology: A prostate strip was isolated, mounted in an organ bath filled with Krebs-Henseleit solution and induced to contract by adrenaline. The contracted strip was then exposed to each extract at 250 µg/mL for 30 minutes. The tension was recorded. Relaxant efficacies of various extracts were determined in prostate strips pre-contracted by adrenaline at 10 µM. All extracts were also determined for their bioactive components and the contents of total phenolics and total flavonoids. Results: The results showed that all of the extracts, as well as tamsulosin, a synthetic drug, exhibited relaxant effects (P < 0.001) on prostate smooth muscles. The UEA exhibited the most potency in relaxing the prostate smooth muscle with a maximal effect of 72.09 ± 2.15 %. The half-maximal effective concentration (EC50) values of the UEA, CEOH, UEOH and CEA were 140.23 ± 9.74, 226.35 ± 7.16, 235.35 ± 24.96 and 236.24 ± 5.05 µg/ml respectively, while tamsulosin was 86.83 ± 8.96 µg/ml. All extracts contained flavonoids, phenolics, sterols, tannins, phlobatannins, terpenoids, cardiac glycosides, alkaloids and reducing sugars. The highest contents of phenolics and flavonoids were found in CEOH and CEA respectively. Conclusion: We concluded that the ethyl acetate from the stems of U. rufa was the most potent in relaxing the prostate smooth muscles, and it may be useful to relieve the urological symptoms caused by benign prostatic hyperplasia (BPH).

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In vitro analysis of antispasmodic activity of ethanolic stem extracts of Uvaria rufa Blume and Anomianthus dulcis (Dunal) J. Sinclair on excised rat’s ileum

Cited by 5 publications

References 18 publications

Anti-mitotic Activities of Ethanolic Extract and Glutinol from Uvaria rufa Blume

Anti-mitotic Activities of Ethanolic Extract and Glutinol from Uvaria rufa Blume

(R)-3-(8'-Hydroxyfarnesyl)-indole and other chemical constituents from the flowers of Anomianthus dulcis and their antimalarial and cytotoxic activities

Relaxant Activities of Extracts from Uvaria rufa Blume and Caesalpinia sappan L. on Excised Rat’s Prostate Strips

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