In vitro analysis: inhibitory effect of Phyllanthus niruri extract against Aeromonas salmonicida

Abstract

The purpose of this research is to explore effectiveness from every part of Phyllanthus niruri such as leaves (L), fruits (F), stem (S) and combination (C) of those parts that able to inhibit Aeromonas salmonicida growth by using different methods of extraction both infundation and maceration. Experiments has been done at Budidaya Perairan Laboratory, Sriwijaya University. In vitro analysis used tetracycline 30 µg/ml as positive control, bacterial culture at 10 14 CFU/ml and concentration of each part of stone breaker was 20%, 40%, 60%, 80% and 100%. The result revealed that stone breaker extract produced by infundation and maceration methods exhibited inhibitory activity against A.salmonicida. Moreover, only maceration method had bactericidal effect, in fact fruits (F) and combination (C) was the best part of P. niruri which had higher inhibition zone than control (antibiotics). It was 16,9 mm and 17,3 mm respectively. However, the extracts are recommended to cure aeromonad infection due to bacterial resistance of tetracycline already increase. Thus, some extraction methods are needed to be developed for further research with minimum inhibition zone is 20 mm.

Combination of Antibacterial Activity of Ethanol Extract of Meniran Leaves and Kenikir Leaves Against Shigella dysenteriae

“…The Meniran leaf ethanolic extract contained: saponins, tannins, flavonoids, polyphenols, and alkaloids (Table 5). This was in accordance with several previous studies that meniran leaves contained saponins, tannins, flavonoids [12], alkaloids [10,16], polyphenols [14]. A previous study has shown that Kenikir leaves contained saponins, tannins, alkaloids, flavonoids, and polyphenols [20,[35][36][37], following the present study (Table 5).…”

“…The condenser was mounted on a rounded bottom flask, containing the solution. This method was carried out three times in circulation, with no water dripping on the scale tube, occurred for 15 min [12].…”

“…Several plants which have medicinal and antibacterial potentials are Meniran (Phyllanthus niruri L.) [10] and Kenikir (Cosmos caudatus Kunth) [11]. Meniran plants can grow wild in the tropical climate and have been used as an antibacterial agent [12]. Also, Meniran has been widely used as an antiviral, an anti-tumor, an anti-carcinogenic [13], an anti-inflammatory, an antioxidant, a hepatoprotection agent, an immunomodulator, and an antifungal [14].…”