In vitro anaerobic biofilms of human colonic microbiota

Sproule-Willoughby, Kimberley M.; Stanton, M. Mark; Rioux, Kevin P.; McKay, Derek M.; Buret, André G.

doi:10.1016/j.mimet.2010.09.020

Cited by 22 publications

(7 citation statements)

References 16 publications

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“…(3) Tryptic soy yeast broth (TSYb, Conda, Italy), prepared according to Sproule-Willoughby et al 23 with the addition of D-glucose to reach 0.5% concentration.…”

Section: Agnp Stability In Solutionmentioning

confidence: 99%

Effects of sublethal concentrations of silver nanoparticles on Escherichia coli and Bacillus subtilis under aerobic and anaerobic conditions

et al. 2016

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The present work is aimed at comparing the effects of sublethal concentrations of silver nanoparticles (AgNPs) on the growth kinetic, adhesion ability, oxidative stress, and phenotypic changes of model bacteria (Escherichia coli and Bacillus subtilis) under both aerobic and anaerobic conditions. Growth kinetic tests conducted in 96-well microtiter plates revealed that sublethal concentrations of AgNPs do not affect E. coli growth, whereas 1 μg/ml AgNPs increased B. subtilis growth rate under aerobic conditions. At the same concentration, AgNPs promoted B. subtilis adhesion, while it discouraged E. coli attachment to the surface in the presence of oxygen. As determined by 2,7-dichlorofluorescein-diacetate assays, AgNPs increased the formation of intracellular reactive oxygen species, but not at the highest concentrations, suggesting the activation of scavenging systems. Finally, motility assays revealed that 0.01 and 1 μg/ml AgNPs, respectively, promoted surface movement in E. coli and B. subtilis under aerobic and anaerobic conditions. The results demonstrate that E. coli and B. subtilis react differently from AgNPs over a wide range of sublethal concentrations examined under both aerobic and anaerobic conditions. These findings will help elucidate the behavior and impact of engineered nanoparticles on microbial ecosystems.

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“…(3) Tryptic soy yeast broth (TSYb, Conda, Italy), prepared according to Sproule-Willoughby et al 23 with the addition of D-glucose to reach 0.5% concentration.…”

Section: Agnp Stability In Solutionmentioning

confidence: 99%

Effects of sublethal concentrations of silver nanoparticles on Escherichia coli and Bacillus subtilis under aerobic and anaerobic conditions

et al. 2016

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“…Then, we reported results on the development of a multi-species biofilm in the lumen of clogged biliary stents as consequence of the ascending colonization from duodenum of aerobic and anaerobic bacteria (Guaglianone et al, 2008). More recently, Howard Ceri group proposed a model for culturing mucosal anaerobic bacteria recovered from colonic biopsies to develop multi-species biofilms (Sproule-Willoughby et al, 2010). The identification by conventional and molecular techniques of both culturable and nonculturable sessilegrowing bacterial and fungal species in the biliary sludge has revealed the occurrence of anaerobes in the 57% of the examined biliary stents and has confirmed the polymicrobial nature of the biofilm developing in their lumen (Guaglianone et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Biofilm-growing intestinal anaerobic bacteria

Donelli

Vuotto

Cardines

et al. 2012

FEMS Immunol Med Microbiol

129

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Sessile growth of anaerobic bacteria from the human intestinal tract has been poorly investigated, so far. We recently reported data on the close association existing between biliary stent clogging and polymicrobial biofilm development in its lumen. By exploiting the explanted stents as a rich source of anaerobic bacterial strains belonging to the genera Bacteroides, Clostridium, Fusobacterium, Finegoldia, Prevotella, and Veillonella, the present study focused on their ability to adhere, to grow in sessile mode and to form in vitro mono- or dual-species biofilms. Experiments on dual-species biofilm formation were planned on the basis of the anaerobic strains isolated from each clogged biliary stent, by selecting those in which a couple of anaerobic strains belonging to different species contributed to the polymicrobial biofilm development. Then, strains were investigated by field emission scanning electron microscopy and confocal laser scanning microscopy to reveal if they are able to grow as mono- and/or dual-species biofilms. As far as we know, this is the first report on the ability to adhere and form mono/dual-species biofilms exhibited by strains belonging to the species Bacteroides oralis, Clostridium difficile, Clostridium baratii, Clostridium fallax, Clostridium bifermentans, Finegoldia magna, and Fusobacterium necrophorum.

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“…The hypothesis was that this approach would provide for a different view of the microbes in this tailings pond environment in comparison with previously published studies. The Calgary Biofilm Device (CBD) has been used to establish mixed species biofilms from colonoscopy sections that closely reflect the diversity of microbial populations seen in individual patients (Sproule-Willoughby et al, 2010). We therefore investigated whether the CBD can be similarly used to obtain representative mixed species biofilms from tailings ponds environments.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of microbial biofilm communities from an Alberta oil sands tailings pond

Golby

Ceri

Gieg

et al. 2011

FEMS Microbiol Ecol

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Bitumen extraction from the oil sands of Alberta has resulted in millions of cubic meters of waste stored on-site in tailings ponds. Unique microbial ecology is expected in these ponds, which may be key to their bioremediation potential. We considered that direct culturing of microbes from a tailings sample as biofilms could lead to the recovery of microbial communities that provide good representation of the ecology of the tailings. Culturing of mixed species biofilms in vitro using the Calgary Biofilm Device (CBD) under aerobic, microaerobic, and anaerobic growth conditions was successful both with and without the addition of various growth nutrients. Denaturant gradient gel electrophoresis and 16S rRNA gene pyrotag sequencing revealed that unique mixed biofilm communities were recovered under each incubation condition, with the dominant species belonging to Pseudomonas, Thauera, Hydrogenophaga, Rhodoferax, and Acidovorax. This work used an approach that allowed organisms to grow as a biofilm directly from a sample collected of their environment, and the biofilms cultivated in vitro were representative of the endogenous environmental community. For the first time, representative environmental mixed species biofilms have been isolated and grown under laboratory conditions from an oil sands tailings pond environment and a description of their composition is provided.

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In vitro anaerobic biofilms of human colonic microbiota

Cited by 22 publications

References 16 publications

Effects of sublethal concentrations of silver nanoparticles on Escherichia coli and Bacillus subtilis under aerobic and anaerobic conditions

Effects of sublethal concentrations of silver nanoparticles on Escherichia coli and Bacillus subtilis under aerobic and anaerobic conditions

Biofilm-growing intestinal anaerobic bacteria

Evaluation of microbial biofilm communities from an Alberta oil sands tailings pond

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