In‐vitro Aluminum Determination and Preconcentration in Blood of Dialysis Patients Based on Ionic Liquid Dispersive Liquid‐Liquid Biomicroextraction by 2‐Amino‐3‐(1H‐imidazol‐4‐yl)propanoic Acid

Abstract

In this study, trace amounts of aluminum in serum of dialysis patients were chelated with 2-Amino-3-(1H-imidazol-4-yl)propanoic acid (Histidine) and determined by electro-thermal atomic absorption spectrometry (ETAAS). A fast and efficient method based on ionic liquid dispersive liquid-liquid bio-micro-extraction (IL-DLLBME) was developed for the determination of Al cation in human blood serum samples. In this work, a small amount of 1-Hexyl-3-methylimmidazolum hexafluorophosphate ([HMIM] [PF 6 ]) as an extractant solvent was dissolved in acetone as a dispersant solvent and then the binary solution was rapidly injected by a syringe into the serum containing Al 3+ ,Which have already in-vitro chelated by Histidine amino acid (Al-His) at pH = 6.5. After separation, the settled IL-phase was dissolved in ethanol up to 200 µL and 20 mL of samples injected into the ET-AAS by auto-sampler. Various parameters have been studied and optimized for 10 mL of sample. Under the optimum conditions, the enrichment factor (EF), limit of detection (LOD) and working range (peak area mode) were obtained 53, 15 ng L -1 and 0.05-4.1 mg L -1 respectively. In vitro Al chelation showed that His can significantly decrease aluminum concentration in serum of dialysis patients. Validation of methodology was confirmed by standard reference material (SRM).

Aluminum separation from drinking water and serum samples based on djenkolic acid immobilized on the multi walled carbon nanotubes by ultrasound-assisted dispersive micro solid phase extraction

“…Also, sample preparation was needed for separating of contaminations from water and biological samples. For this purpose, the vary preconcentration/separation techniques such as liquid-liquid extraction (LLE), dispersive liquidliquid microextraction (DLLME) [16,17], and solid phase extraction (SPE) [18] was applied. Recently, the SPE methods were used as a suitable technique for extraction aluminum as compared to others.…”