In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteins

Willer, Emerson da Motta; Lima, Renato de Lourenço; Giugliano, Loreny Gimenes

doi:10.1186/1471-2180-4-18

Cited by 34 publications

(13 citation statements)

References 33 publications

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“…The importance of the sugar residues on Lf is suggested by the observation that whereas native hLf inhibits Shigella spp. adhesion [ 92 ], recombinant hLf (rhLf), with different glycosylation, has no effect on Shigella flexneri adhesion to epithelial cells [ 93 ]. Another paper suggests that hLf, rhLf and bLf inhibit the attachment of Helicobacter felix to gastric epithelial cells, probably by interaction between oligomannoside-type glycans of Lf and bacterial adhesins that recognize these residues [ 94 ].…”

Section: Lactoferrinmentioning

confidence: 99%

Lactoferrin: A Natural Glycoprotein Involved in Iron and Inflammatory Homeostasis

Rosa

Cutone

Lepanto

et al. 2017

IJMS

286

277

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Human lactoferrin (hLf), an iron-binding multifunctional cationic glycoprotein secreted by exocrine glands and by neutrophils, is a key element of host defenses. HLf and bovine Lf (bLf), possessing high sequence homology and identical functions, inhibit bacterial growth and biofilm dependently from iron binding ability while, independently, bacterial adhesion to and the entry into cells. In infected/inflamed host cells, bLf exerts an anti-inflammatory activity against interleukin-6 (IL-6), thus up-regulating ferroportin (Fpn) and transferrin receptor 1 (TfR1) and down-regulating ferritin (Ftn), pivotal actors of iron and inflammatory homeostasis (IIH). Consequently, bLf inhibits intracellular iron overload, an unsafe condition enhancing in vivo susceptibility to infections, as well as anemia of inflammation (AI), re-establishing IIH. In pregnant women, affected by AI, bLf oral administration decreases IL-6 and increases hematological parameters. This surprising effect is unrelated to iron supplementation by bLf (80 μg instead of 1–2 mg/day), but to its role on IIH. AI is unrelated to the lack of iron, but to iron delocalization: cellular/tissue overload and blood deficiency. BLf cures AI by restoring iron from cells to blood through Fpn up-expression. Indeed, anti-inflammatory activity of oral and intravaginal bLf prevents preterm delivery. Promising bLf treatments can prevent/cure transitory inflammation/anemia/oral pathologies in athletes.

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Section: Lactoferrinmentioning

confidence: 99%

Lactoferrin: A Natural Glycoprotein Involved in Iron and Inflammatory Homeostasis

Rosa

Cutone

Lepanto

et al. 2017

IJMS

286

277

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“…However, limited studies have revealed that in the intestinal parts of rats that are abundant in microbiota, such as the colon, the presence of DMT1 on enterocytes is elevated, which can ameliorate Fe gut absorption [33]. Lactoferrin, an Fe-binding glycoprotein, is able to decrease adhesion and invasion of Shigella, responsible for colon disorders [34]. Moreover, it has been documented that the intestinal microbiota regulates the gut expression of DCYTB, DMT1, TfR, and ferritin genes in epigenetic mode [15].…”

Section: Introductionmentioning

confidence: 99%

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

et al. 2020

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The influence of probiotic supplementation on iron metabolism remains poorly investigated. However, a range of studies, especially on Lactobacillus plantarum 299v (Lp229v), have indicated a possible positive impact of probiotics on iron absorption. The aim of the study was to determine the effect of multistrain probiotic supply on iron balance. Thirty Wistar rats were randomized into three groups: placebo (KK group), and multistrain probiotic per os in a daily dose of 2.5 × 109 colony forming units (CFU) (PA group) or 1 × 1010 CFU (PB group). Multistrain probiotic consisted of nine bacterial strains: Bifidobacterium bifidum W23, B. lactis W51, B. lactis W52, Lactobacillus acidophilus W37, L. brevis W63, L. casei W56, L. salivarius W24, Lactococcus lactis W19, and Lc. lactis W58, in equal proportions. After six weeks, blood and organ samples were collected. No differences were found between the three groups in terms of serum concentrations of hepcidin (HEPC), lactoferrin (LTF), homocysteine (HCY), ferritin (Ft), or erythroferrone (ErFe), or in liver content of divalent metal transporter 1 (DMT1), transferrin receptors 1 and 2 (TfR), or ZRT/IRT-like protein 14 (ZIP14) proteins. In the overall sample, positive correlations were noted between the serum concentrations of hepcidin and lactoferrin, and hepcidin and ferritin; serum concentration of hepcidin and DMT1 and TfR1 in the liver; and serum concentration of erythroferrone and TfR2 in the liver. The correlations of serum hepcidin and erythroferrone with liver DMT1 and TfR represent significant mechanisms of Fe homeostasis. Our study has shown that multistrain probiotic supplementation used in the experiment did not disrupt the biochemical and hepatic regulatory processes of Fe balance and did not demonstrate significant influence on selected parameters of Fe metabolism.

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“…Moreover, its cationic charge is responsible for the binding to bacterial and cell surface components (Valenti and Antonini 2005). This Lf property has been shown to inhibit the adhesion and entry into epithelial cells of several facultative intracellular bacteria (Longhi et al 1993;Ajello et al 2002;Di Biase et al 2004;Willer et al 2004;Berlutti et al 2008); however Lf activity against obligate intracellular bacteria as C. trachomatis has never been observed.…”

Section: Discussionmentioning

confidence: 99%

Effect of bovine lactoferrin onChlamydia trachomatisinfection and inflammation

Sessa

Pietro

Filardo

et al. 2017

Biochem. Cell Biol.

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Chlamydia trachomatis is an obligate, intracellular pathogen responsible for the most common sexually transmitted bacterial disease worldwide, causing acute and chronic infections. The acute infection is susceptible to antibiotics, whereas the chronic one needs prolonged therapies, thus increasing the risk of developing antibiotic resistance. Novel alternative therapies are needed. The intracellular development of C. trachomatis requires essential nutrients, including iron. Iron-chelating drugs inhibit C. trachomatis developmental cycle. Lactoferrin (Lf), a pleiotropic iron binding glycoprotein, could be a promising candidate against C. trachomatis infection. Similarly to the efficacy against other intracellular pathogens, bovine Lf (bLf) could both interfere with C. trachomatis entry into epithelial cells and exert an anti-inflammatory activity. In vitro and in vivo effects of bLf against C. trachomatis infectious and inflammatory process has been investigated. BLf inhibits C. trachomatis entry into host cells when incubated with cell monolayers before or at the moment of the infection and down-regulates IL-6/IL-8 synthesized by infected cells. Six out of 7 pregnant women asymptomatically infected by C. trachomatis, after 30 days of bLf intravaginal administration, were negative for C. trachomatis and showed a decrease of cervical IL-6 levels. This is the first time that the bLf protective effect against C. trachomatis infection has been demonstrated.

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In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteins

Cited by 34 publications

References 33 publications

Lactoferrin: A Natural Glycoprotein Involved in Iron and Inflammatory Homeostasis

Lactoferrin: A Natural Glycoprotein Involved in Iron and Inflammatory Homeostasis

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

Effect of bovine lactoferrin onChlamydia trachomatisinfection and inflammation

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