In vitro acute cytotoxicity of abamectin to the Gill Cell Line of Flounder Paralichthy olivaceus

Xu, Yujuan; Guo, Huarong; Xiao, Qin; Su, Feng; Yin, Licheng

doi:10.1007/s11802-007-0369-y

Cited by 4 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar positive results have also been revealed for other target cells. Abamectin was able to induce a significant inhibition of the cell-growth proliferation in a derived gill flounder cell line [Xu et al, 2007]. In other in vivo systems, Abamectin did not induce chromosomal aberrations in the bone marrow cells of mice orally exposed to 1-12 mg/kg dose-range [Blazak et al, 1983].…”

Section: Genotoxicity and Cytotoxicity Of Abamectin And Ivermectinmentioning

confidence: 99%

New Ventures in the Genotoxic and Cytotoxic Effects of Macrocyclic Lactones, Abamectin and Ivermectin

Molinari

Soloneski

Larramendy³

2010

Cytogenet Genome Res

View full text Add to dashboard Cite

Abamectin and Ivermectin are 2 closely related members of the Avermectin family of 16-membered macrocyclic lactones derived from the actinomycete Streptomyces avermectinius which exhibit extraordinary anthelmintic activity. They are used worldwide in veterinary and human medicine as well as in agriculture. In the present review we summarized the results published so far for estimating the genotoxicity and cytotoxicity exerted by both compounds in several cellular systems. Although both compounds do not induce in vitro and in vivo gene mutations in either bacterial or mammalian cells, there is no concrete evidence of a clear clastogenic effect exerted both in vitro and in vivo in mammalian cells. However, reports indicating that both anthelmintic agents are able to induce single DNA-strand breaks in vitro and inhibit cell growth either in vitro or in in vivo bioassays, are scarce. Taking into account the similarity of the genotoxicity and cytotoxicity exerted by both antibiotics, and that only Abamectin has been classified so far as a class II toxicity pesticide by the EPA, the necessity of reconsideration for a further hazard evaluation of Ivermectin by an international regulatory agency(ies) is strongly recommended.

show abstract

Section: Genotoxicity and Cytotoxicity Of Abamectin And Ivermectinmentioning

confidence: 99%

New Ventures in the Genotoxic and Cytotoxic Effects of Macrocyclic Lactones, Abamectin and Ivermectin

Molinari

Soloneski

Larramendy³

2010

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…Here, we describe the development and initial validation of a p21 -mediated luciferase reporter gene bioassay system for genotoxicity detection using a continuous flounder gill cell line (FG). The FG cell line has been established and widely used to study the toxic effects and mechanisms of environmental pollutants on fish species [ 5 , 6 , 40 , 41 , 42 , 43 , 44 , 45 ]. Unlike mammalian cells, FG cells can be easily maintained in a wide range of temperatures from 15 °C to 30 °C.…”

Section: Introductionmentioning

confidence: 99%

Development of a Fish Cell Biosensor System for Genotoxicity Detection Based on DNA Damage-Induced Trans-Activation of p21 Gene Expression

Geng¹,

Zhang²,

Guo³

2012

Biosensors

Self Cite

View full text Add to dashboard Cite

p21CIP1/WAF1 is a p53-target gene in response to cellular DNA damage. Here we report the development of a fish cell biosensor system for high throughput genotoxicity detection of new drugs, by stably integrating two reporter plasmids of pGL3-p21-luc (human p21 promoter linked to firefly luciferase) and pRL-CMV-luc (CMV promoter linked to Renilla luciferase) into marine flatfish flounder gill (FG) cells, referred to as p21FGLuc. Initial validation of this genotoxicity biosensor system showed that p21FGLuc cells had a wild-type p53 signaling pathway and responded positively to the challenge of both directly acting genotoxic agents (bleomycin and mitomycin C) and indirectly acting genotoxic agents (cyclophosphamide with metabolic activation), but negatively to cyclophosphamide without metabolic activation and the non-genotoxic agents ethanol and D-mannitol, thus confirming a high specificity and sensitivity, fast and stable response to genotoxic agents for this easily maintained fish cell biosensor system. This system was especially useful in the genotoxicity detection of Di(2-ethylhexyl) phthalate (DEHP), a rodent carcinogen, but negatively reported in most non-mammalian in vitro mutation assays, by providing a strong indication of genotoxicity for DEHP. A limitation for this biosensor system was that it might give false positive results in response to sodium butyrate and any other agents, which can trans-activate the p21 gene in a p53-independent manner.

show abstract

In vitro and in vivo acute toxicity of fenpyroximate to flounder Paralichthys olivaceus and its gill cell line FG

Na¹,

Guo²,

Zhang³

et al. 2009

Aquatic Toxicology

View full text Add to dashboard Cite

In vitro acute cytotoxicity of abamectin to the Gill Cell Line of Flounder Paralichthy olivaceus

Cited by 4 publications

References 10 publications

New Ventures in the Genotoxic and Cytotoxic Effects of Macrocyclic Lactones, Abamectin and Ivermectin

New Ventures in the Genotoxic and Cytotoxic Effects of Macrocyclic Lactones, Abamectin and Ivermectin

Development of a Fish Cell Biosensor System for Genotoxicity Detection Based on DNA Damage-Induced Trans-Activation of p21 Gene Expression

In vitro and in vivo acute toxicity of fenpyroximate to flounder Paralichthys olivaceus and its gill cell line FG

Contact Info

Product

Resources

About