In vitro activity of the nonsteroidal anti-inflammatory drug indomethacin on a scuticociliate parasite of farmed turbot

Paramá, A.; Piazzon, M. Carla; Lamas, Jesús; Sanmartín, M. L.; Leiro, J.

doi:10.1016/j.vetpar.2007.06.018

Cited by 11 publications

(12 citation statements)

References 30 publications

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“…have been used to study the efficacy of chemotherapeutic drugs and treatments. As no effective control exists for M. avidus in fish, many potential drugs have been screened for their effects on these ciliates in culture [124,135,166,[172][173][174][175]. Formalin, hydrogen peroxide, and resveratrol were among 17 drugs found to be toxic to the ciliates.…”

Section: Chemotherapymentioning

confidence: 99%

“…However, unlike formalin and resveratrol susceptibility, the susceptibility to hydrogen peroxide varied considerably between different P. dicentrarchi isolates [172]. Other toxic treatments were chitosan microspheres cross linked with glutaraldehyde and containing beta-cyclodextrin [174] and the anti-inflammatory drug indomethacin [175]. Indomethacin reduced the growth of P. dicentrachi and eventually caused cell death, possibly by apoptosis [175].…”

Section: Chemotherapymentioning

confidence: 99%

“…Other toxic treatments were chitosan microspheres cross linked with glutaraldehyde and containing beta-cyclodextrin [174] and the anti-inflammatory drug indomethacin [175]. Indomethacin reduced the growth of P. dicentrachi and eventually caused cell death, possibly by apoptosis [175]. For C. irritans, the double-layered co-culture system with FHM cells was used to study the effects of antiprotozoal compounds on trophonts [176].…”

Section: Chemotherapymentioning

confidence: 99%

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Use of Cell Cultures to Study the Interactions of Ciliates with Fish

Pinheiro¹,

Bols²

2013

Springer Science Reviews

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How the ciliates of fish can be cultured and be used to study ciliate/fish interactions are reviewed. The culturing of ciliates is currently done in basal solutions based on either freshwater, seawater, or bodily fluids of vertebrates. These are supplemented either with bacteria, fish cells, or organic matter, which can be defined or more commonly undefined, with proteose peptone being a prominent example. Among pathogenic ciliates, the most difficult to culture has been Ichthyophthirius multifiliis. To contrast, Cryptocaryon irritans caused a similar disease and has been maintained successfully in co-cultures with fish cells. Pathogenic scuticociliates and tetrahymenas are more amenable to culture, and can be grown axenically. These cultures have been used to study pathogenic mechanisms and to screen drugs for their potential chemotherapeutic value. Ciliates may act directly on fish to cause disease, but may also influence fish health indirectly through their interactions with other types of fish pathogens, for example bacteria and fungi. For example, in culture Tetrahymena spp. have been shown to phagocytose pathogenic bacteria, Yersinia ruckerii, and microsporidia, Glugea hertwigi, where ciliates are not linked to disease, very different ciliate/fish interactions are possible. For some fish larvae, free-living ciliates are a source of nutrients. Large-scale cultures of both freshwater and marine ciliates have been achieved and could be a source of feed for fish larvae in aquaculture. Finally, ciliates have the potential to feed on fish carcasses and in doing so make nutrients available to the ecosystem. In the future cell cultures should be invaluable in studying these and other possible relationships between fish and ciliates.

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Section: Chemotherapymentioning

confidence: 99%

Section: Chemotherapymentioning

confidence: 99%

Section: Chemotherapymentioning

confidence: 99%

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Use of Cell Cultures to Study the Interactions of Ciliates with Fish

Pinheiro¹,

Bols²

2013

Springer Science Reviews

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“…In Korea, epizootics of scuticociliatosis caused by Miamiensis avidus (= Philasterides dicentrarchi) have led to mass mortalities in cultured olive flounder every year (Kim et al, 2004;Jung et al, 2007). Many chemotherapeutics for treatment of scuticociliatosis have been attempted (Iglesias et al, 2002;Quintela et al, 2003;Paramá et al, 2004Paramá et al, , 2007Budiño et al, 2012), but finding effective in vivo therapeutics has failed. Although a high treatment efficacy of feeding doxycycline or bath with freshwater containing doxycycline against scuticociliatosis in olive flounder was demonstrated by our recent study (Kang et al, 2013b), development of immunological control measures is still required not only to enhance survival of fish but also to solve problems related to chemotherapeutics' side-effects.…”

Section: Introductionmentioning

confidence: 99%

Preventive and therapeutic effects of auxotrophic Edwardsiella tarda mutant harboring CpG 1668 motif-enriched plasmids against scuticociliatosis in olive flounder (Paralichthys olivaceus)

Kang

Choi

Kim

2014

Experimental Parasitology

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“…The ciliates not only infect external skin or gill but also penetrate into all internal organs including brain [1][2][3]. Although trials to treat scuticociliatosis using various chemotherapeutants have been made [4][5][6], there is at present no effective in vivo chemotherapeutant especially for systemic infections. Therefore, development of preventive measures using vaccines or immunostimulants would be the best way to limit the occurrence of scuticociliatosis in cultured fish.…”

Section: Introductionmentioning

confidence: 99%