In Vitro Activity of the Antifungal Plant Defensin RsAFP2 against
            <i>Candida</i>
            Isolates and Its In Vivo Efficacy in Prophylactic Murine Models of Candidiasis

Tavares, Patricia M.; Thevissen, Karin; Cammue, Bruno P. A.; François, Isabelle; Barreto‐Bergter, Eliana; Taborda, Carlos Pelleschi; Marques, Alexandre F.; Rodrigues, Márcio L.; Nimrichter, Leonardo

doi:10.1128/aac.00448-08

Cited by 73 publications

(60 citation statements)

References 27 publications

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“…RsAFP2 is not toxic to mammalian cells and is prophylactically effective against murine candidiasis [20]. RsAFP2 interacts with GlcCer (glucosylceramide) in the plasma membrane of susceptible fungi.…”

Section: Rsafp2mentioning

confidence: 99%

Apoptosis-inducing antifungal peptides and proteins

Brucker

Cammue

Thevissen

2011

Biochemical Society Transactions

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Despite the availability of various classes of antimycotics, the treatment of patients with systemic fungal infections is challenging. Therefore the development of new antifungals is urgently required. Promising new antifungal candidates are antimicrobial peptides. In the present review, we provide an overview of antifungal peptides isolated from plants, insects, amphibians and mammals that induce apoptosis. Their antifungal spectrum, mode of action and toxicity are discussed in more detail.

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Section: Rsafp2mentioning

confidence: 99%

Apoptosis-inducing antifungal peptides and proteins

Brucker

Cammue

Thevissen

2011

Biochemical Society Transactions

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“…sphingolipids) and modulation of intracellular signaling cascades (Aerts et al 2008). RsAFP2 was also effective in an in vivo prophylactic model of murine candidiasis (Tavares et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Antifungal and antitumor models of bioactive protective peptides

Rodrigues

Dobroff

Taborda

et al. 2009

An. Acad. Bras. Ciênc.

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Peptides are remarkably reactive molecules produced by a great variety of species and able to display a number of functions in uni-and multicellular organisms as mediators, agonists and regulating substances. Some of them exert cytotoxic effects on cells other than those that produced them, and may have a role in controlling subpopulations and protecting certain species or cell types. Presently, we focus on antifungal and antitumor peptides and discuss a few models in which specific sequences and structures exerted direct inhibitory effects or stimulated a protective immune response. The killer peptide, deduced from an antiidiotypic antibody, with several antimicrobial activities and other Ig-derived peptides with cytotoxic activities including antitumor effects, are models studied in vitro and in vivo. Peptide 10 from gp43 of P. brasiliensis (P10) and the vaccine perspective against paracoccidioidomycosis is another topic illustrating the protective effect in vivo against a pathogenic fungus. The cationic antimicrobial peptides with antitumor activities are mostly reviewed here. Local treatment of murine melanoma by the peptide gomesin is another model studied at the Experimental Oncology Unit of UNIFESP.

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“…However, more complicated mechanisms also exist, involving uptake into the cytoplasm and action within the target cell (9). In the case of fungal pathogens, several antimicrobial peptides have been investigated for inhibitory activity, including human betadefensins, histatin 5, LL-37, and plant defensins (10)(11)(12). The mechanisms by which these polypeptides act against C. albicans vary considerably.…”

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confidence: 99%

Identification and Mechanism of Action of the Plant Defensin NaD1 as a New Member of the Antifungal Drug Arsenal against Candida albicans

Hayes

Bleackley

Wiltshire

et al. 2013

Antimicrob Agents Chemother

103

165

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bIn recent decades, pathogenic fungi have become a serious threat to human health, leading to major efforts aimed at characterizing new agents for improved treatments. Promising in this context are antimicrobial peptides produced by animals and plants as part of innate immune systems. Here, we describe an antifungal defensin, NaD1, with activity against the major human pathogen Candida albicans, characterize the mechanism of killing, and identify protection strategies used by the fungus to survive defensin treatment. The mechanism involves interaction between NaD1 and the fungal cell surface followed by membrane permeabilization, entry into the cytoplasm, hyperproduction of reactive oxygen species, and killing induced by oxidative damage. By screening C. albicans mutant libraries, we identified that the high-osmolarity glycerol (HOG) pathway has a unique role in protection against NaD1, while several other stress-responsive pathways are dispensable. The involvement of the HOG pathway is consistent with induction of oxidative stress by NaD1. The HOG pathway has been reported to have a major role in protection of fungi against osmotic stress, but our data indicate that osmotic stress does not contribute significantly to the adverse effects of NaD1 on C. albicans. Our data, together with previous studies with human beta-defensins and salivary histatin 5, indicate that inhibition of the HOG pathway holds promise as a broad strategy for increasing the activity of antimicrobial peptides against C. albicans.

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In Vitro Activity of the Antifungal Plant Defensin RsAFP2 against Candida Isolates and Its In Vivo Efficacy in Prophylactic Murine Models of Candidiasis

Cited by 73 publications

References 27 publications

Apoptosis-inducing antifungal peptides and proteins

Apoptosis-inducing antifungal peptides and proteins

Antifungal and antitumor models of bioactive protective peptides

Identification and Mechanism of Action of the Plant Defensin NaD1 as a New Member of the Antifungal Drug Arsenal against Candida albicans

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