In vitro activity of the new water-dispersible Fe3O4@usnic acid nanostructure against planktonic and sessile bacterial cells

MAPLE fabrication of thin films based on kanamycin functionalized magnetite nanoparticles with anti-pathogenic properties

“…Fe 3 O 4 @KAN was prepared according to our previously published papers [12,13,14,15]. A c c e p t e d M a n u s c r i p t deposition, the target was kept at a temperature of ~173 K by active liquid nitrogen cooling.…”

Design and characterization of antimicrobial usnic acid loaded-core/shell magnetic nanoparticles

“…Particularly, these systems exhibited improved antimicrobial activity against E. faecalis (MIC ≅ 1 μg/mL) and E. coli (MIC ≅ 10 μg/mL) as compared with F 3 O 4 [44]. In our opinion, these very low MIC values were probably related to the significant amount of loaded usnic acid as a consequence of the adopted synthetic procedure.…”

“…Better results have been obtained in the literature by Grumezescu et al [44] on water-dispersible F 3 O 4 -UA magnetic nanoparticles without polymer shell. Particularly, these systems exhibited improved antimicrobial activity against E. faecalis (MIC ≅ 1 μg/mL) and E. coli (MIC ≅ 10 μg/mL) as compared with F 3 O 4 [44].…”

“…Some studies have investigated the UA entrapping in micro-or nano-carriers underlying the applicative potential of these controlled releasing systems [40][41][42]. Usnic acid has been also recently adsorbed onto the surface of oleic acid coated-magnetite and tested in terms of its ability to prevent biofilm development in S. aureus, E. coli, P. aeruginosa and Enterococcus faecalis [43,44]. Results showed how magnetite/UA nanoparticles exhibited efficient antimicrobial activity against planktonic and adherent cells of Gram-positive strains.…”

Nano-Coupling of Cephalosporin Antibiotics with Fe<SUB>3</SUB>O<SUB>4</SUB> Nanoparticles: Trojan Horse Approach in Antimicrobial Chemotherapy of Infections Caused by <i>Klebsiella spp</i>.

“…In order to measure antibacterial effect on biofilm development, the nutrient broth was deleted; wells were washed with PBS three times and stained by crystal violet 1% for 15 minutes. The biofilm, formed onto the plastic wells, was re-suspended in 30% acetic acid and the intensity of the colored suspension was assayed by measuring the absorbance at 590 nm [15]- [17]. All microbiological studies were performed 4 times in order to exclude mistakes and to prove reproducibility.…”