In vitro activity of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts against Leishmania major in Iran

Abstract

Leishmania is transmitted by sandflies that ingest the parasite in the amastigote phase resident within macrophages, then inoculate the promastigote phase into body hosts. The present study was conducted to evaluate the in vitro effects of alcoholic extract of Plants on L.major. The extract of aerial parts of plants were obtained by maceration. The in vitro experiments were performed on promastigotes to assess antileishmanial activity of the extracts using glucantime as 194 Fatemeh Tabatabaie et al.a reference. The extract of plants and glucantime solutions for biological testing were prepared in PBS at 0.05-0.1-0.2-0.4 μg/mL and 1μg/mL, respectively. All experiments were repeated at least three times in duplicate. For the extract of plants and glucantime, the concentration-response curve was plotted, from which IC50 values were determined also No and MTT assay were done. The different concentrations resulted in different optical densities or inhibitory percentages (P<0.05) so that extract of plants were effective against L. major in vitro. NO production the extract of plants showed significant in vitro antileishmanial activities.The Findings of this study indicate that these plants are effective against L.major in vitro.

Dithallium(III)-Containing 30-Tungsto-4-phosphate, [Tl2Na2(H2O)2(P2W15O56)2]16–: Synthesis, Structural Characterization, and Biological Studies

“…Diseases caused by Leishmania are a continuous health problem worldwide due to the emergence of resistance developed by protozoans. Several drugs like meglumine antimoniate, potassium antimonial tartrate, and Amphotericin B are being used as first-line treatments against leishmaniasis, but drug resistance is a major challenge . Therefore, the design of new antileishmanial drugs having less resistance is desperately needed.…”

“…Several drugs like meglumine antimoniate, potassium antimonial tartrate, and Amphotericin B are being used as first-line treatments against leishmaniasis, but drug resistance is a major challenge. 19 Therefore, the design of new antileishmanial drugs having less resistance is desperately needed. In the current work, we explored the consequences of Tl-POMs at an equimolar concentration of thallium against promastigotes of Leishmania tropica and compared it with Amphotericin B.…”

Comparison of Hyssopus officinalis, Tussilage farfara, Carum copticum extracts versus systemic glucantime in the treatment of cutaneous leishmaniasis in Balb/c mice

“…In each test groups (10%), the lesions in 2 animals, out of 10, disappeared completely. The possible mechanism for this effect is through release of nitric acid and tumor necrosis factor from macrophages (10).In another study, peganine exhibited in vitro activity against both extracellular promastigotes as well as intracellular amastigotes residing within murine macrophages in L. donovani then P harmala seeds extract showed significant in vitro and in vivo antileishmanial activities (16). The results of this study mentioned above is in agreement with the results of our study.…”

“…It was found that a 200-mg amount of ointment was used per mouse per day using cotton applicator. After the end of the treatment period, mice in the experimental and placebo groups were followed for 1 month (10). The Nitrit levels were measured by the Griess reaction, which is an assay for NO production.Peritoneal macrophages were harvested.…”