In Vitro Activity of Fluoroquinolones Against Ocular Bacterial Isolates in São Paulo, Brazil

Oliveira, Adália Dias Dourado; d’Azevedo, Pedro Alves; Francisco, Waldemar

doi:10.1097/01.ico.0000248379.78777.f6

Cited by 33 publications

(31 citation statements)

References 21 publications

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“…Considering the meticulous scraping technique followed as a routine in the study, however, the shown high prevalence of CoNS point to CoNS as a main CLMK agent in our environment. Oxacillin resistance was not found among CoNS in the present study, though increasing prevalence has been observed among general eye infections in Brazil (29)(30)(31) . Oxacillin resistance is used to evaluate the resistance of Staphylococcus to beta-lactam agents.…”

Section: Discussionmentioning

confidence: 38%

Contact lens-associated microbial keratitis

Moriyama¹,

Hofling-Lima²

2008

Arq. Bras. Oftalmol.

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Section: Discussionmentioning

confidence: 38%

Contact lens-associated microbial keratitis

Moriyama¹,

Hofling-Lima²

2008

Arq. Bras. Oftalmol.

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“…15,40–45 For in vitro studies, the minimum inhibitory concentrations for 90% of bacterial isolates (MIC 90 ) can be used as relative measure of efficacy for release of antibiotics. All of the CLs, in all testing conditions, released enough moxifloxacin to meet the MIC 90 (0.047 μg/mL) 46 against common and susceptible pathogens over the 24-hour study period.…”

Section: Discussionmentioning

confidence: 99%

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Phan

Bajgrowicz-Cieslak

Subbaraman

et al. 2016

Trans. Vis. Sci. Tech.

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PurposeThe aim of this study was to evaluate and compare the release of moxifloxacin from a variety of daily disposable (DD) contact lenses (CLs) under various conditions using a novel in vitro eye model.MethodsFour commercially available DD conventional hydrogel (CH) CLs (nelfilcon A, omafilcon A, etafilcon A, and ocufilcon B) and three silicone hydrogel (SH) CLs (somofilcon A, narafilcon A, and delefilcon A) were evaluated. These lenses were incubated in moxifloxacin for 24 hours. The release of the drug was measured using a novel in vitro model in three experimental conditions: (1) phosphate buffered saline (PBS); (2) artificial tear solution (ATS) containing a variety of proteins and lipids; and (3) ATS with mechanical rubbing produced by the device.ResultsOverall, CH CLs had a higher drug release than SH CLs (P < 0.05) under all conditions. Typically, a higher drug release was observed in PBS than ATS (P < 0.05). For CH, drug release was found to be higher in ATS with rubbing than PBS or ATS (P < 0.05). For most lens types, ATS with rubbing produced higher drug release than ATS alone (P < 0.05). Generally, the release kinetics for all conditions were sustained over the 24-hour testing period, and no burst release was observed (P < 0.05).ConclusionsMoxifloxacin release from a CL into ATS is lower when compared to release into PBS. When mechanical rubbing is introduced, the amount of drugs released is increased.Translational RelevanceResults suggest that sophisticated in vitro models are necessary to adequately model on-eye drug release from CL materials.

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“…Sixty-four per cent of those who include this in their treatment regime prefer ciprofloxacin. Moxifloxacin has been shown to provide better Gram-positive coverage 23 24. The most common bacteria involved in blebitis are mainly Gram-positive bacteria— Staphylococcus epidermidis, Staphylococcus aureus and Streptococcus pneumoniae —which are consistent with the normal flora on the eye 25 26.…”

Section: Discussionmentioning

confidence: 99%