In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG) versus multidrug-resistant Acinetobacter baumannii

Betts, Jonathan W.; Wareham, David W.

doi:10.1186/1471-2180-14-172

Cited by 93 publications

(56 citation statements)

References 32 publications

(40 reference statements)

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“…The combination was clearly bactericidal, and significant synergy was produced, indicated by a >3 log CFU/mL difference between the agents used in combination versus alone. 18 The data presented here with the exception of P. aeruginosa agree with previous results, whereby 2 alone showed no antibacterial activity over 24 h, in time−kill assays. 18 This could be due to the insolubility of 2 or the inability of the compound to pass through the outer cell membrane/cell wall to reach its target.…”

supporting

confidence: 94%

In Vitro Antibacterial Activity of Curcumin–Polymyxin B Combinations against Multidrug-Resistant Bacteria Associated with Traumatic Wound Infections

Betts

Sharili²,

Ragione

et al. 2016

J. Nat. Prod.

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Bacterial infections resulting from nonsurgical traumatic wounds can be life threatening, especially those caused by multidrug-resistant (MDR) bacteria with limited therapeutic options. The antimicrobial activity of polymyxin B (1) and curcumin (2) alone and in combination was determined versus MDR bacterial isolates associated with traumatic wound infections. Cytotoxicity assays for 1 and 2 were undertaken in keratinocyte cell lines. Minimum inhibitory concentrations of 1 were significantly reduced in the presence of 2 (3- to 10-fold reduction), with synergy observed. Time-kill assays showed the combinations produced bactericidal activity. Cytotoxicity assays indicate the toxicity of 2 was reduced in the presence of 1.

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supporting

confidence: 94%

In Vitro Antibacterial Activity of Curcumin–Polymyxin B Combinations against Multidrug-Resistant Bacteria Associated with Traumatic Wound Infections

Betts

Sharili²,

Ragione

et al. 2016

J. Nat. Prod.

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“…These compounds provide health benefits and show remarkable antimicrobial activity against resistant pathogens [13, 14]. Tea extracts having high concentrations of epigallocatechin gallate (EGCG) and epigallocatechin (EGC) are more potent in antibacterial activity against bacterial pathogens [15].…”

Section: Introductionmentioning

confidence: 99%

Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa - Combination therapy against resistant bacteria -

Bazzaz¹,

Sarabandi²,

Khameneh³

et al. 2016

Journal of Pharmacopuncture

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Objectives:Bacterial resistant infections have become a global health challenge and threaten the society’s health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus (S. aureus) and the standard strain of Pseudomonas aeruginosa (P. aeruginosa).Methods:The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied in vitro by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI).Results:The MICs of gentamicin against bacterial strains were in the range of 0.312 - 320 μg/mL. The MIC values of both types of catechins were 62.5 - 250 μg/ mL. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin.Conclusion:The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and standard P. aeruginosa strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.

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“…Due to high content of phenolic compounds, especially flavan-3-ols and phenolic acids, this plant has become very interesting as a source of antimicrobial substances. So far, it was shown that /-/-epigallocatechin gallate, present in great burnet extracts, in combination with curcumin (isolated from Curcuma longa rhizomes) is very effective against multidrug-resistant Acinetobacter baumannii [13]. It was confirmed that extracts form this plant have strong effect against avian E. coli [14].…”

Section: Ta B L Ementioning

confidence: 89%

Accumulation of biomass and phenolic compounds in Polish and Mongolian great burnet (Sanguisorba officinalis L.) populations

Bączek

2014

Herba Polonica

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S u m m a r yEight Polish and six Mongolian great burnet populations were evaluated ex situ in respect of the mass of underground organs and accumulation of biologically active compounds. In the raw materials collected in autumn of the second year of plant vegetation, total content of tannins and phenolic acids as well as the content of phenolic compounds identified by HPLC were determined. Distinct differences between Polish and Mongolian populations and their high intraspecific variability, concerning analysed parameters, was observed. The mass of underground organs of Polish populations was higher than in Mongolian ones (595.0 and 523.5 g × plant -1 , respectively, for fresh mass). Polish populations were characterised by significantly higher total content of tannins and phenolic acids (6.02 and 1.60%, respectively) in comparison with Mongolian ones (2.89 and 0.97%, respectively). In the investigated raw materials eight phenolic compounds were identified, namely: /-/-epigallocatechin, /+/-catechin, /-/-epicatechin, /-/-epicatechin gallate, /-/-epigallocatechin gallate, astragalin, ellagic and gallic acids. In all populations, the dominating compound, was /-/-epigallocatechin. The contents of /-/-epigallocatechin, /-/-epicatechin gallate and gallic acid were distinctly higher in Polish populations. INTRODuCTIONGreat burnet (Sanguisorba officinalis L., Rosaceae), is a perennial growing wild on wet grasslands, hillside meadows and woodland in Europe, Asia and northern regions of North America [1]. In Poland it occurs mostly in southern regions, whereas in Mongolia it is spread through the whole country. The raw material collected from this plant are underground organs (rhizomes and roots) known for its healing properties [1][2][3][4]. They are used in the treatment of fever, bleeding, diarrhoea, haemorrhoids and burns. In traditional Chinese medicine it is said to cool the blood, clear heat and heal wounds [3][4][5][6]. The active compounds responsible for the biological activity of great burnet roots are polyphenol compounds, in particular flavan-3-ols and phenolic acids as well as sterols and saponin compounds [1][2][3]7]. The herb of great burnet is sometimes used as a medicinal raw material too. Fresh leaves of young plants are added as a seasoning to salads and meat dishes in some western countries as well [1]. So far, the raw materials of great burnet for medicinal purposes have been collected almost exclusively from wild growing plants, which are genetically and chemically differentiated [5,8].The aim of undertaken study was to investigate, in ex situ conditions, the chemical diversity of 14 great burnet populations originating both from Poland and from Mongolia. MATERIALS AND METhODS Plant materialEight populations of great burnet originating from southern part of Poland (PL) and six from northern Mongolia (M) were the object of the study (tab. 1). At each wild growing population, both in Poland and Mongolia, the seeds were collected in the autumn, 2009. The species identification was made according to ...

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In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG) versus multidrug-resistant Acinetobacter baumannii

Cited by 93 publications

References 32 publications

In Vitro Antibacterial Activity of Curcumin–Polymyxin B Combinations against Multidrug-Resistant Bacteria Associated with Traumatic Wound Infections

In Vitro Antibacterial Activity of Curcumin–Polymyxin B Combinations against Multidrug-Resistant Bacteria Associated with Traumatic Wound Infections

Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa - Combination therapy against resistant bacteria -

Accumulation of biomass and phenolic compounds in Polish and Mongolian great burnet (Sanguisorba officinalis L.) populations

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