In vitro activity of ciprofloxacin, sparfloxacin, ofloxacin, amikacin and rifampicin against Ghanaian isolates of Mycobacterium ulcerans

Thangaraj, Harry; Adjei, Ohene; Allen, B.W.; Portaels, F.; Evans, M.R.W.; Banerjee, D.; Wansbrough‐Jones, Mark

doi:10.1093/jac/45.2.231

Cited by 63 publications

(59 citation statements)

References 7 publications

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“…The method used to evaluate the antibacterial activity also affected the results. Fazeli et al, (2007) 47 founded that the diffusion method from wells on agar was more suitable for studying the activity of aqueous, organic and hydro-ethanolic plant extracts than the agar diffusion method. In addition, the effect of a plant substance depends on several factors including the mode of extraction and concentration of active compound.…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…In addition, the effect of a plant substance depends on several factors including the mode of extraction and concentration of active compound. 47 According to the study of Bagre, 2007, 48 some chemical groups are eliminated and others are concentrated during the liquid-liquid extraction. Thus, the alkaloids and sterols that were less concentrated in the aqueous total extract were strongly present in the acetate extract while the concentration of flavonoids remained regardless of the solvent used.…”

Section: Antibacterial Activitymentioning

confidence: 99%

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Composition and Antibacterial Activity of Hydro-Alcohol and Aqueous Extracts Obtained from the Lamiaceae Family

Ramdan¹,

Fatima²,

Kamal³

et al. 2017

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Context:Plant from the Lamiaceae family are considered as dietary spices and medicinal herbs traditionally used in medicine for the treatment of several pathologies. Objective: Evaluation of the in vitro antibacterial activity of ethanol and aqueous extracts of nine Moroccan plants from the Lamiaceae family against six bacterial strains regularly implicated in toxiinfection. Method: The antibacterial activities of hot (HAE), cold (CAE) aqueous extracts and ethanolic extracts (EE) were evaluated using agar-well diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and IC 50 against six foodborne bacteria (Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica). Total phenolic and flavonoid contents were assessed as well. Results: In the present study, the ethanol extracts were rich in polyphenols, with middling values of flavonoids, and relatively poor in condensed tannins. The presence of saponins, essential oils, irroides, alkaloids, anthocyanins, and aldehydes was recorded. The effect of the extracts was directly bactericidal for B. cereus and L. monocytogenes; for the other strains, the MBC value was twice higher than that of MIC. However, the ethanol extract (EE) of O. majorana and T. satureioides showed the highest antibacterial activity. With the principal component analysis, it was demonstrated that the nine Lamiaceae family plants possess a powerful antibacterial effect, correlated with their phenolic content. Statistical analysis : Analysis of variance was performed by uni-varied ANOVA in the software SPSS 22 Fr. Conclusion : The active compounds were thermostable and soluble in ethanol and water. The antimicrobial activities of the plants extracts investigated may contribute to understand their involvement in traditional medicine against many microbial infections.

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Section: Antibacterial Activitymentioning

confidence: 99%

Section: Antibacterial Activitymentioning

confidence: 99%

Composition and Antibacterial Activity of Hydro-Alcohol and Aqueous Extracts Obtained from the Lamiaceae Family

Ramdan¹,

Fatima²,

Kamal³

et al. 2017

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“…ulcerans has been shown in vitro to be susceptible to rifampin (17), aminoglycosides, macrolides (25), and quinolones (30). It was susceptible to the same drugs in the mouse footpad model (7,11,29), but clarithromycin and quinolones were bacteriostatic whereas rifampin, amikacin, and streptomycin appeared to be bactericidal (10); the size of mouse footpad lesions treated with rifampin and amikacin together for 12 weeks decreased progressively, the mean CFU counts of M. ulcerans were reduced, and there were no relapses (10,21).…”

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confidence: 99%

Efficacy of the Combination Rifampin-Streptomycin in Preventing Growth of Mycobacterium ulcerans in Early Lesions of Buruli Ulcer in Humans

Etuaful

Carbonnelle

Grosset

et al. 2005

Antimicrob Agents Chemother

219

212

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Mycobacterium ulcerans disease is common in some humid tropical areas, particularly in parts of West Africa, and current management is by surgical excision of skin lesions ranging from early nodules to extensive ulcers (Buruli ulcer). Antibiotic therapy would be more accessible to patients in areas of Buruli ulcer endemicity. We report a study of the efficacy of antibiotics in converting early lesions (nodules and plaques) from culture positive to culture negative. Lesions were excised either immediately or after treatment with rifampin orally at 10 mg/kg of body weight and streptomycin intramuscularly at 15 mg/kg of body weight daily for 2, 4, 8, or 12 weeks and examined by quantitative bacterial culture, PCR, and histopathology for M. ulcerans. Lesions were measured during treatment. Five lesions excised without antibiotic treatment and five lesions treated with antibiotics for 2 weeks were culture positive, whereas three lesions treated for 4 weeks, five treated for 8 weeks, and three treated for 12 weeks were culture negative. No lesions became enlarged during antibiotic treatment, and most became smaller. Treatment with rifampin and streptomycin for 4 weeks or more inhibited growth of M. ulcerans in human tissue, and it provides a basis for proceeding to a trial of antibiotic therapy as an alternative to surgery for early M. ulcerans disease.

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“…In vitro, M. ulcerans is susceptible to several antimicrobials, including rifampin (RIF), clarithromycin (CLR), streptomycin (STR), amikacin (AMK), sparfloxacin (SPX), and clofazimine (1,11,13,14,19). However, the clinical response of humans with BU to various antimicrobial regimens containing these drugs have not confirmed in vitro studies so that, to date, BU treatment still relies on extensive surgical excision of the lesions followed by skin grafting (20).…”

mentioning

confidence: 99%

Activities of New Macrolides and Fluoroquinolones against Mycobacterium ulcerans Infection in Mice

Bentoucha

Robert

Dega

et al. 2001

Antimicrob Agents Chemother

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Mice infected in the left hind footpad with 5 log 10 acid-fast bacilli of Mycobacterium ulcerans were divided into an untreated control group and 17 treatment groups that received one of the following regimens for 4 weeks (all doses in milligrams per kilogram): 100 mg of azithromycin (AZM), 100 mg of clarithromycin (CLR), or 50 mg of AZM for a duration of 5 days a week (daily), three times a week, or once weekly. In addition, the following regimens were administered daily: 100 mg of telithromycin (TLM), sparfloxacin (SPX), or moxifloxacin (MOX); 200 mg of levofloxacin (LVX); 100 mg of streptomycin (STR) or amikacin (AMK); 10 mg of rifampin (RIF); and the combination of 10 mg of RIF and 100 mg of AMK (RIF؉AMK). After completion of treatment, mice were observed for 30 weeks. The effectiveness of treatment regimens was assessed in terms of the delay in median time to footpad swelling in treated mice compared with that in the untreated controls. Clear-cut bactericidal activity, i.e., an observed delay in footpad swelling that exceeded the period of treatment, was observed in the STR-, AMK-, and RIF؉AMK-treated mice. However, all mice treated with either AMK or STR alone had swollen footpads before the end of the 30-week observation period, suggesting regrowth of M. ulcerans. In contrast, 50% of the mice treated with the RIF؉AMK combination exhibited no lesion even after 30 weeks, suggesting cure. The remaining regimens could be assigned to one of three groups: (i) no activity (50 mg of AZM, 100 mg of AZM thrice weekly, TLM, and LVX); (ii) bacteriostatic activity, i.e., a delay in footpad swelling shorter than the 4-week treatment duration (100 mg of AZM daily or once weekly, CLR thrice or once weekly, and MOX); or (iii) weak bactericidal activity (CLR daily and SPX). The RIF؉AMK combination and possibly RIF؉STR warrant further study for the treatment of M. ulcerans infection in humans.Mycobacterium ulcerans, the only toxin-producing mycobacterial species (5) is responsible for Buruli ulcer (BU), the third most prevalent mycobacterial disease throughout the world (20). Over the past 10 years, an increasing incidence of BU has been reported in several West African countries (6, 8) and Australia (4) and is now reported in most of sub-Saharan Africa, Central and South America, India, Southeast Asia, and Papua New Guinea (20).In vitro, M. ulcerans is susceptible to several antimicrobials, including rifampin (RIF), clarithromycin (CLR), streptomycin (STR), amikacin (AMK), sparfloxacin (SPX), and clofazimine (1,11,13,14,19). However, the clinical response of humans with BU to various antimicrobial regimens containing these drugs have not confirmed in vitro studies so that, to date, BU treatment still relies on extensive surgical excision of the lesions followed by skin grafting (20).In a previous study, the mouse footpad model of Fenner (3) was used to assess the in vivo activity of several drugs against M. ulcerans (2). CLR, for which in vitro activity against M. ulcerans has been demonstrated (11), exhibited only bacteri...

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In vitro activity of ciprofloxacin, sparfloxacin, ofloxacin, amikacin and rifampicin against Ghanaian isolates of Mycobacterium ulcerans

Cited by 63 publications

References 7 publications

Composition and Antibacterial Activity of Hydro-Alcohol and Aqueous Extracts Obtained from the Lamiaceae Family

Composition and Antibacterial Activity of Hydro-Alcohol and Aqueous Extracts Obtained from the Lamiaceae Family

Efficacy of the Combination Rifampin-Streptomycin in Preventing Growth of Mycobacterium ulcerans in Early Lesions of Buruli Ulcer in Humans

Activities of New Macrolides and Fluoroquinolones against Mycobacterium ulcerans Infection in Mice

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