In vitro activity of antifungal and antiseptic agents against dermatophyte isolates from patients with tinea pedis

Abstract

The in vitro activity of antifungal and antiseptic agents were evaluated against dermatophytes isolated from patients with tinea pedis. The antifungals studied were: ciclopirox olamine, cetoconazole, tolciclate and terbinafine, and the antiseptics were: povidine iodine (PVPI), propolis, Fungol®, Andriodermol®, and boric acid. The minimum inhibitory concentration (MIC) or the minimal dilution concentration (MDC) was determined by an agar dilution method using modified yeast nitrogen agar base, and the minimum fungicidal concentration (MFC) or minimum fungicidal dilution (MFD) was determined with subcultures on Sabouraud dextrose agar. All drugs studied were active against the dermatophytes at lower concentrations than those used in products and/or pharmaceutical preparations for topical use. Some antifungal agents, mainly terbinafine and tolciclate, presented higher efficacy than the other drugs, with lower MICs and MFCs values. It was concluded that the use of these antiseptic drugs represent an excellent alternative for the topical treatment of tinea pedis. For the treatment of severe cases these are the antifungal agents of choice.

In vitro antibacterial activities of ethanol extract of iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas hydrophila, Yersinia ruckeri & Streptococcus iniae)

“…Propolis is a very complex mixture and, in general, it is composed of 50% balsams and resins, 30% wax, 10% essential oils, 5% pollen and 5% of various other substances like sugars, vitamins, etc (16,30). Bees modify propolis by -glucodiases, Therefore, the aim of the present study was to investigate the in vitro antimicrobial activity of ethanol extract of propolis from…”

Avaliação do método de disco-difusão para determinação da eficácia da terbinafina in vitro em agentes de micoses superficiais e subcutâneas

“…2 However, the appearance of resistant fungi established the need to research different therapy alternatives and action sites. 3 Terbinafine is among the drugs that have an action mechanism different from that of azoles. It belongs to the family of alilamines, acts on the epoxidase enzyme of the fungal cell and is especially indicated to treat skin infections caused by dermatophytes.…”

“…4 Contrary to imidazoles, terbinafine has an antifungal action. 1,2,3 Terbinafine is more selective for the fungal cell than amphotericin B, a standard antifungal agent, due to a difference between the epoxidase enzymes of mammals and fungi. 5 Terbinafine can be administered in combination with other drugs and presents mild toxic effects or adverse reactions.…”

Resposta in vitro de fungos agentes de micoses cutâneas frente aos antifúngicos sistêmicos mais utilizados na dermatologia

“…9 Muitas dessas drogas pertencem ao mesmo grupo de ação farmacológica e possuem o mesmo mecanismo de ação; contudo, os fungos respondem de forma significativamente diferente quanto à susceptibilidade a elas. 10,11 Por ser complexa a escolha apropriada do antifúngico, é necessá-rio conhecer informações sobre o próprio fungo, bem como sua interação com as drogas antifúngicas. 12 No entanto, o tratamento é usualmente prescrito de forma empírica, sem a confirmação micológica e muitas vezes ministrado por um tempo inadequado de seguimento.…”