In vitro activity and stability of pure human salivary aldehyde dehydrogenase

Laskar, Amaj Ahmed; Alam, Md. Fazle; Younus, Hina

doi:10.1016/j.ijbiomac.2016.12.084

“…With pre‐heating and dilution, the signals were recovered, with optimal signals obtained at 25 % dilution, and the trends were consistent with those observed in buffer (Figure S8b). It is speculated that pre‐heating would minimize the interference caused by saliva components, such as mucins, [37] enzymes, [38] and other proteins, [39] while preserving target binding [40] . The use of a heat step for viral testing in saliva has been used in previous works [24,41] and can be achieved using compact flexible heaters that are powered using portable electrochemical potentiostats compatible with point‐of‐need testing [42] …”

Section: Resultsmentioning

confidence: 99%

High‐Precision Viral Detection Using Electrochemical Kinetic Profiling of Aptamer‐Antigen Recognition in Clinical Samples and Machine Learning

Sen,

Zhang,

Sakib

et al. 2024

Angewandte Chemie

0

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High‐precision viral detection at point of need with clinical samples plays a pivotal role in the diagnosis of infectious diseases and the control of a global pandemic. However, the complexity of clinical samples that often contain very low viral concentrations makes it a huge challenge to develop simple diagnostic devices that do not require any sample processing and yet are capable of meeting performance metrics such as very high sensitivity and specificity. Herein we describe a new single‐pot and single‐step electrochemical method that uses real‐time kinetic profiling of the interaction between a high‐affinity aptamer and an antigen on a viral surface. This method generates many data points per sample, which when combined with machine learning, can deliver highly accurate test results in a short testing time. We demonstrate this concept using both SARS‐CoV‐2 and Influenza A viruses as model viruses with specifically engineered high‐affinity aptamers. Utilizing this technique to diagnose COVID‐19 with 37 real human saliva samples results in a sensitivity and specificity of both 100% (27 true negatives and 10 true positives, with 0 false negative and 0 false positive), which showcases the superb diagnostic precision of this method.

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“…With pre‐heating and dilution, the signals were recovered, with optimal signals obtained at 25 % dilution, and the trends were consistent with those observed in buffer (Figure S8b). It is speculated that pre‐heating would minimize the interference caused by saliva components, such as mucins, [37] enzymes, [38] and other proteins, [39] while preserving target binding [40] . The use of a heat step for viral testing in saliva has been used in previous works [24,41] and can be achieved using compact flexible heaters that are powered using portable electrochemical potentiostats compatible with point‐of‐need testing [42] …”

Section: Resultsmentioning

confidence: 99%

High‐Precision Viral Detection Using Electrochemical Kinetic Profiling of Aptamer‐Antigen Recognition in Clinical Samples and Machine Learning

Sen,

Zhang,

Sakib

et al. 2024

Angew Chem Int Ed

2

0

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High‐precision viral detection at point of need with clinical samples plays a pivotal role in the diagnosis of infectious diseases and the control of a global pandemic. However, the complexity of clinical samples that often contain very low viral concentrations makes it a huge challenge to develop simple diagnostic devices that do not require any sample processing and yet are capable of meeting performance metrics such as very high sensitivity and specificity. Herein we describe a new single‐pot and single‐step electrochemical method that uses real‐time kinetic profiling of the interaction between a high‐affinity aptamer and an antigen on a viral surface. This method generates many data points per sample, which when combined with machine learning, can deliver highly accurate test results in a short testing time. We demonstrate this concept using both SARS‐CoV‐2 and Influenza A viruses as model viruses with specifically engineered high‐affinity aptamers. Utilizing this technique to diagnose COVID‐19 with 37 real human saliva samples results in a sensitivity and specificity of both 100% (27 true negatives and 10 true positives, with 0 false negative and 0 false positive), which showcases the superb diagnostic precision of this method.

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“…This enzyme stimulate (give-and-take) of electron among the (donor and acceptor) molecule, reaction comprising electrons transferal, protons, Hydrogen extractive, Hydride transfer, Oxygen insert, also extra significant stages [1,2]. Generally, two in half reaction such as some oxidative and one reduction occurring and at smallest two substrate such as one reduces and one oxidize is activate and convert [3]. Oxidoreductases comprise of a great categorize of enzyme catalyze the transmission of electron from an electrons donor (reduction) to an electron acceptor (oxidation) molecules, general take NADP nicotinamide-adenine-dinucleotide phosphate or NAD nicotinamide -adenine-dinucleotide as cofactor (Figure 1) [4].…”

Section: Enzymes Oxido-reductasesmentioning

confidence: 99%

Oxidoreductases: Significance for Humans and Microorganism

Kareem¹

2021

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Oxidoreductases consist of a large class of enzymes catalyzing the transfer of electrons from an electron donor (reductant) to an electron acceptor (oxidant) molecule. Since so many chemical and biochemical transformations comprise oxidation/reduction processes, it has long been an important goal in biotechnology to develop practical biocatalytic applications of oxidoreductases. During the past few years, significant breakthrough has been made in the development of oxidoreductase-based diagnostic tests and improved biosensors, and the design of innovative systems for the regeneration of essential coenzymes. Research on the construction of bioreactors for pollutants biodegradation and biomass processing, and the development of oxidoreductase-based approaches for synthesis of polymers and functionalized organic substrates have made great progress. Proper names of oxidoreductases are in a form of “donor:acceptor oxidoreductase”; while in most cases “donor dehydrogenase” is much more common. Common names also sometimes appeared as “acceptor reductase”, such as NAD+ reductase. “Donor oxidase” is a special case when O2 serves as the acceptor. In biochemical reactions, the redox reactions are sometimes more difficult to observe, such as this reaction from glycolysis: Pi + glyceraldehyde-3-phosphate + NAD+ → NADH + H+ + 1,3-bisphosphoglycerate, where NAD+ is the oxidant (electron acceptor), and glyceraldehyde-3-phosphate functions as reductant (electron donor).

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In vitro activity and stability of pure human salivary aldehyde dehydrogenase

Cited by 17 publications

References 54 publications

High‐Precision Viral Detection Using Electrochemical Kinetic Profiling of Aptamer‐Antigen Recognition in Clinical Samples and Machine Learning

High‐Precision Viral Detection Using Electrochemical Kinetic Profiling of Aptamer‐Antigen Recognition in Clinical Samples and Machine Learning

High‐Precision Viral Detection Using Electrochemical Kinetic Profiling of Aptamer‐Antigen Recognition in Clinical Samples and Machine Learning

Oxidoreductases: Significance for Humans and Microorganism

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