In vitro Actinomyces israelii biofilm development on IUD copper surfaces

Carrillo, Mónica; Valdez, Benjamín; Osuna, Lidia Vargas; Álvarez, Lydia; Schorr, M.; Zlatev, Roumen; Stoytcheva, Margarita

doi:10.1016/j.contraception.2009.09.008

Cited by 17 publications

(16 citation statements)

References 15 publications

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“…The organisms existing in such colonies can collectively evade destruction and elimination by host phagocytic cells. A recent study demonstrated the capacity of A. israelii to produce EPSs and to form biofilm in vitro on copper surfaces of intrauterine contraceptive devices immersed in a simulated uterine fluid under anaerobic conditions (Carrillo et al 2010). Actinomyces radicidentis is known to form biofilms, in which bacteria seem to be embedded in the extracellular matrix and exhibit antiphagocytic activity against rat neutrophils (Nair et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of exopolysaccharide‐producing Actinomyces oris isolated from an apical abscess lesion

et al. 2012

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Aim To demonstrate a capacity for producing exopolysaccharides (EPSs) and an ability to form biofilm on abiotic materials of Actinomyces oris strain K20. Methodology The productivity of EPSs and the ability to form biofilm of strain K20 were evaluated by measuring viscosity of spent culture media and by scanning electron microscopy (SEM) and the biofilm assay on microtitre plates, respectively. High‐performance liquid chromatography was used to determine the chemical composition of the viscous materials. To examine the role of the viscous materials attributable to the pathogenicity in this organism, the ability of strain K20 to induce abscess formation was compared in mice to that of ATCC 27044. Results The viscosity of the spent culture media of K20 was significantly higher than that of ATCC 27044. Strain K20 showed dense meshwork structures around the cells and formed biofilms on microtitre plates, whereas ATCC 27044 did not. Chemical analysis of the viscous materials revealed that they were mainly composed of neutral sugars with mannose constituting 77.5% of the polysaccharides. Strain K20 induced persistent abscesses in mice lasting at least 5 days at a concentration of 108 cells mL−1, whereas abscesses induced by ATCC 27044 healed and disappeared or decreased in size at day 5. Conclusions Strain K20 produced EPSs, mainly consisting of mannose, and formed biofilms. This phenotype might play an important role for A. oris to express virulence through the progression of apical periodontitis.

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of exopolysaccharide‐producing Actinomyces oris isolated from an apical abscess lesion

et al. 2012

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“…Among 130 Actinomyces isolates from clinical material associated with IUCDs sent to a reference laboratory for identification to the species level, one-third were identified as A. israelii, with its prevalence being double those of A. turicensis, A. naeslundii, A. odontolyticus, and A. gerencseriae, which were the next most common species (64). In an in vitro study, A. israelii grown in synthetic intrauterine medium was demonstrated to attach to and form spiderlike colonies and porous biofilm structures on copper plates, where the presence of sulfur was also confirmed (123). A. israelii has been found in IUCD users with pelvic manifestations (124,125).…”

Section: Abdominal/pelvic Actinomycosismentioning

confidence: 99%

Actinomyces and Related Organisms in Human Infections

2015

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SUMMARY Actinomyces israelii has long been recognized as a causative agent of actinomycosis. During the past 3 decades, a large number of novel Actinomyces species have been described. Their detection and identification in clinical microbiology laboratories and recognition as pathogens in clinical settings can be challenging. With the introduction of advanced molecular methods, knowledge about their clinical relevance is gradually increasing, and the spectrum of diseases associated with Actinomyces and Actinomyces -like organisms is widening accordingly; for example, Actinomyces meyeri , Actinomyces neuii , and Actinomyces turicensis as well as Actinotignum (formerly Actinobaculum ) schaalii are emerging as important causes of specific infections at various body sites. In the present review, we have gathered this information to provide a comprehensive and microbiologically consistent overview of the significance of Actinomyces and some closely related taxa in human infections.

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“…Depending on the site of infection, companion organisms are commonly coisolated [3]. A frequent association with foreign objects (eg, pelvic actinomycosis and intrauterine contraceptive devices, pulmonary actinomycosis, and foreign body aspiration) suggests growth facilitation by biofilm formation [4]. …”

Section: Discussionmentioning

confidence: 99%