In vitro 3D Full‐Thickness Skin‐Equivalent Tissue Model Using Silk and Collagen Biomaterials

Bellas, Evangelia; Seiberg, Miri; Garlick, Jonathan A.; Kaplan, David L.

doi:10.1002/mabi.201200262

Cited by 114 publications

(97 citation statements)

References 14 publications

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“…After demonstrating the ability of our nanoprobes to sense Vimentin mRNA in both confluent and migrating cells, we moved to a more complex structure using a damaged skin tissue model. Various types of tissue models have been used in different studies to investigate wound healing . For our studies, dorsal murine skin of new‐born mice was injected with Vimentin DNA–gold nanoprobes.…”

Section: Resultsmentioning

confidence: 99%

Sensing of Vimentin mRNA in 2D and 3D Models of Wounded Skin Using DNA‐Coated Gold Nanoparticles

Vilela

Heuer‐Jungemann

El‐Sagheer

et al. 2018

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Wound healing is a highly complex biological process, which is accompanied by changes in cell phenotype, variations in protein expression, and the production of active biomolecules. Currently, the detection of proteins in cells is done by immunostaining where the proteins in fixed cells are detected by labeled antibodies. However, immunostaining cannot provide information about dynamic processes in living cells, within the whole tissue. Here, an easy method is presented to detect the transition of epithelial to mesenchymal cells during wound healing. The method employs DNA-coated gold nanoparticle fluorescent nanoprobes to sense the production of Vimentin mRNA expressed in mesenchymal cells. Fluorescence microscopy is used to achieve temporal detection of Vimentin mRNA in wounds. 3D light-sheet microscopy is utilized to observe the dynamic expression of Vimentin mRNA spatially around the wounded site in skin tissue. The use of DNA-gold nanoprobes to detect mRNA expression during wound healing opens up new possibilities for the study of real-time mechanisms in complex biological processes.

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Section: Resultsmentioning

confidence: 99%

Sensing of Vimentin mRNA in 2D and 3D Models of Wounded Skin Using DNA‐Coated Gold Nanoparticles

Vilela

Heuer‐Jungemann

El‐Sagheer

et al. 2018

Small

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“…Traditional monolayer skin equivalents used to study these processes often fail to mimic normal human skin barrier functions and properties due to excluded cell types and improper force application, thereby limiting physiological relevance [179]. As an improvement to traditional monolayer skin equivalents, 3D human skin equivalents better recapitulate natural skin tissue composition and function with a combination of endothelial cells, adipose tissue and immune cells added to the full-thickness skin models [181,182]. For example, Bellas et al introduced a 3D full-thickness skin-equivalent in vitro model using silk and collagen as scaffolds.…”

Section: Skin-on-a-chipmentioning

confidence: 99%

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Shi

Wang

et al. 2019

Biomaterials

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A B S T R A C TBacterial infections and antibiotic resistant bacteria have become a growing problem over the past decade. As a result, the Centers for Disease Control predict more deaths resulting from microorganisms than all cancers combined by 2050. Currently, many traditional models used to study bacterial infections fail to precisely replicate the in vivo bacterial environment. These models often fail to incorporate fluid flow, bio-mechanical cues, intercellular interactions, host-bacteria interactions, and even the simple inclusion of relevant physiological proteins in culture media. As a result of these inadequate models, there is often a poor correlation between in vitro and in vivo assays, limiting therapeutic potential. Thus, the urgency to establish in vitro and ex vivo systems to investigate the mechanisms underlying bacterial infections and to discover new-age therapeutics against bacterial infections is dire. In this review, we present an update of current in vitro and ex vivo models that are comprehensively changing the landscape of traditional microbiology assays. Further, we provide a comparative analysis of previous research on various established organ-disease models. Lastly, we provide insight on future techniques that may more accurately test new formulations to meet the growing demand of antibiotic resistant bacterial infections. Lack of adequate model systemsCommonly, a variety of inconsistent, in vitro and in vivo models have been progressively utilized for antibiotic/drug development and pathophysiological studies. These systems range from simple in vitro models using microtiter plate assays and flow cells to more complex in https://doi.

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“…However, the environment of melanoma in patients is even more complex, comprising of more cell types, such as immune cells, endothelial cells and adipocytes. Some authors have developed full thickness HSE, including the hypodermal components and Langerhans cells forming a tri‐layered structure which mimics the full spectrum of biological functions of the real skin . A more complex MSE could allow to study the impact of soluble factors or cytokines released from tumor cells or surrounding cells on melanoma invasion, drug response in a context that is more closed to in vivo.…”

Section: Melanoma 3d Modelsmentioning

confidence: 99%

Progress in melanoma modelling in vitro

Marconi

Quadri

Saltari

et al. 2018

Experimental Dermatology

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Melanoma is one of the most studied neoplasia, although laboratory techniques used for investigating this tumor are not fully reliable. Animal models may not predict the human response due to differences in skin physiology and immunity. In addition, international guidelines recommend to develop processes that contribute to the reduction, refinement and replacement of animals for experiments (3Rs). Adherent cell culture has been widely used for the study of melanoma to obtain important information regarding melanoma biology. Nonetheless, these cells grow in adhesion on the culture substrate which differs considerably from the situation in vivo. Melanoma grows in a 3D spatial conformation where cells are subjected to a heterogeneous exposure to oxygen and nutrient. In addition, cell-cell and cell-matrix interaction play a crucial role in the pathobiology of the tumor as well as in the response to therapeutic agents. To better study, melanoma new techniques, including spherical models, tumorospheres and melanoma skin equivalents, have been developed. These 3D models allow to study tumors in a microenvironment that is more close to the in vivo situation and are less expensive and time-consuming than animal studies. This review will also describe the new technologies applied to skin reconstructs such as organ-on-a-chip that allows skin perfusion through microfluidic platforms. 3D in vitro models, based on the new technologies, are becoming more sophisticated, representing at a great extent the in vivo situation, the "perfect" model that will allow less involvement of animals up to their complete replacement, is still far from being achieved.

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In vitro 3D Full‐Thickness Skin‐Equivalent Tissue Model Using Silk and Collagen Biomaterials

Cited by 114 publications

References 14 publications

Sensing of Vimentin mRNA in 2D and 3D Models of Wounded Skin Using DNA‐Coated Gold Nanoparticles

Sensing of Vimentin mRNA in 2D and 3D Models of Wounded Skin Using DNA‐Coated Gold Nanoparticles

In vitro and ex vivo systems at the forefront of infection modeling and drug discovery

Progress in melanoma modelling in vitro

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