In vitro 3D angiogenesis assay in egg white matrix: comparison to Matrigel, compatibility to various species, and suitability for drug testing

Mousseau, Yoanne; Mollard, Séverine; Qiu, Hao; Richard, Laurence; Cazal, R; Nizou, Angélique; Védrenne, Nicolas; Rémi, Séverine; Baaj, Yasser; Fourcade, Laurent; Funalot, Benoît; Sturtz, Franck

doi:10.1038/labinvest.2013.150

Cited by 25 publications

(21 citation statements)

References 34 publications

(35 reference statements)

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“…Especially Matrigel is known for its proangiogenic properties that allow for the fast formation of a vascular‐like network. Due to this reason it is an often used, highly potent material for vasculogenesis/angiogenesis‐related assays …”

Section: Discussionmentioning

confidence: 99%

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Klotz

Oosterhoff

Utomo

et al. 2019

Adv Healthcare Materials

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For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG‐peptide approaches, can efficiently support tissue development from human primary cells.

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Section: Discussionmentioning

confidence: 99%

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Klotz

Oosterhoff

Utomo

et al. 2019

Adv Healthcare Materials

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“…E2 sponges promoted infiltration and proliferation of HDFs during in vitro culture, leading to the production and deposition of extracellular matrix. The extent of attachment, migration, and proliferation of different cell types has been reported to be enhanced by proteins derived from egg white . This ability was mirrored in vivo by efficient cell colonization and engraftment of the construct with surrounding tissue .…”

Section: Discussionmentioning

confidence: 98%

Facile Fabrication of Egg White Macroporous Sponges for Tissue Regeneration

Baharvand

Rajabi-Zeleti

Mohammadi

et al. 2015

Adv Healthcare Materials

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The availability of 3D sponges combining proper biochemical, biophysical, and biomechanical properties with enhanced capacity of in vivo engraftment and vascularization is crucial in regenerative medicine. A simple process is developed to generate macroporous scaffolds with a well-defined architecture of interconnected pores from chicken egg white (EW), a material with protein- and growth factor-binding features which has not yet been employed in regenerative medicine. The physicomechanical properties and degradation rates of the scaffold are finely tuned by using varying concentrations of the cross-linker, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, without alteration of the biochemical traits. In vitro, EW scaffolds supported active metabolism, proliferation, and migration of human dermal fibroblasts, thereby generating uniform cellular constructs. In vivo, subcutaneous implantation in mice reveals negligible immune reaction and efficient cell and tissue ingrowth. Angiogenesis into EW scaffolds is enhanced as compared to standard collagen type I sponges used as reference material, likely due to significantly higher adsorption of the proangiogenic factor vascular endothelial growth factor. In summary, a material is presented derived by facile processing of a highly abundant natural product. Due to the efficient subcutaneous engraftment capacity, the sponges can find utilization for soft tissue regeneration.

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“…The effect of compound 3c in the invasive property of MCF-7 cell was measured by a wellestablished matrigel cell invasion assay. 29 It was observed that with increase in concentration of compound 3c the numbers of invaded colonies were significantly decreased with respect to untreated control. This indicates that compound 3c inhibited the in vitro cell invasion ( Figure S5).…”

Section: Acs Medicinal Chemistry Lettersmentioning

confidence: 94%

Scaffold-Hopping of Aurones: 2-Arylideneimidazo[1,2-a]pyridinones as Topoisomerase IIα-Inhibiting Anticancer Agents

Priyadarshani

Nayak

Amrutkar

et al. 2016

ACS Med. Chem. Lett.

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Scaffold-hopping of bioactive natural product aurones has been studied for the first time. 2-Arylideneimidazo[1,2-a]-pyridinones as potential topoisomerase IIα (hTopoIIα)-targeting anticancer compounds were considered. A multifunctional activator, polyphosphoric acid, enabled to realize a cascade reaction of 2-aminopyridine with 2,3-epoxyesters toward synthesis of 2-arylideneimidazo[1,2-a]pyridinones. Most of the compounds exhibited hTopoIIα-selective poison activity with efficiency more than etoposide and DNA-binding property, while not interacting with hTopo I. The compounds showed pronounced antiproliferative activities in nanomolar range with relatively poor toxicity to normal cells, inhibition of invasiveness, and apoptotic effect. The activities for inhibition of tubulin assembly, CDK1 and pCDK1, were also observed. Interestingly, the hTopoIIα inhibitory (in vitro and ex vivo studies) and antiproliferative activities of representative potent compounds were found to be manifold higher compared to corresponding parent aurones bearing alike substitutions, indicating the importance of such scaffold-hopping strategy in medicinal chemistry research.

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In vitro 3D angiogenesis assay in egg white matrix: comparison to Matrigel, compatibility to various species, and suitability for drug testing

Cited by 25 publications

References 34 publications

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Facile Fabrication of Egg White Macroporous Sponges for Tissue Regeneration

Scaffold-Hopping of Aurones: 2-Arylideneimidazo[1,2-a]pyridinones as Topoisomerase IIα-Inhibiting Anticancer Agents

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