In utero exposure to caffeine causes delayed neural tube closure in rat embryos

Wilkinson, Jenny M.; Pollard, Irina

doi:10.1002/tcm.1770140502

Cited by 27 publications

(17 citation statements)

References 24 publications

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“…In another study using rat embryos, a significantly higher number of neural tubes failed to close in the caudal region of the caffeine-treated embryos (91%) in comparison to the control (14%) [21]. In keeping with these studies, we showed that high concentrations of caffeine exposure led to the formation of chick NTD (Fig.…”

Section: Discussionsupporting

confidence: 83%

Exploring the Caffeine-Induced Teratogenicity on Neurodevelopment Using Early Chick Embryo

Yang

Wang

et al. 2012

PLoS ONE

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Caffeine consumption is worldwide. It has been part of our diet for many centuries; indwelled in our foods, drinks, and medicines. It is often perceived as a “legal drug”, and though it is known to have detrimental effects on our health, more specifically, disrupt the normal fetal development following excessive maternal intake, much ambiguity still surrounds the precise mechanisms and consequences of caffeine-induced toxicity. Here, we employed early chick embryos as a developmental model to assess the effects of caffeine on the development of the fetal nervous system. We found that administration of caffeine led to defective neural tube closures and expression of several abnormal morphological phenotypes, which included thickening of the cephalic mesenchymal tissues and scattering of somites. Immunocytochemistry of caffeine-treated embryos using neural crest cell markers also demonstrated uncharacteristic features; HNK1 labeled migratory crest cells exhibited an incontinuous dorsal-ventral migration trajectory, though Pax7 positive cells of the caffeine-treated groups were comparatively similar to the control. Furthermore, the number of neurons expressing neurofilament and the degree of neuronal branching were both significantly reduced following caffeine administration. The extent of these effects was dose-dependent. In conclusion, caffeine exposure can result in malformations of the neural tube and induce other teratogenic effects on neurodevelopment, although the exact mechanism of these effects requires further investigation.

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Section: Discussionsupporting

confidence: 83%

Exploring the Caffeine-Induced Teratogenicity on Neurodevelopment Using Early Chick Embryo

Yang

Wang

et al. 2012

PLoS ONE

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“…Similar results have been reported by other researchers: West et al [34] (caffeine administered in doses of 50 mg/kg and 75 mg/kg body weight between the 3 rd and 19 th day of gestation), Muther [20] (caffeine administered in a dose of 100 mg/kg body weight during pregnancy), Wilkinson and Pollard [37] (caffeine administered in a dose of 25 mg/kg on the 8 th and 9 th day of gestation) and Nakamoto et al [21] (caffeine administered in dosses between 0.5-2 mg/kg between the 8 th and 22 nd day of gestation). They found that caffeine in these doses administered to pregnant females results in foetuses being lighter.…”

Section: Discussionsupporting

confidence: 91%

“…Experimental research has shown that in high doses it can cause an embryotoxic effect. In smaller doses it reduces the weight gain of pregnant females, reduces the number of offspring per litter as well as foetal morphometric parameters (it interferes with intrauterine growth) [4,20,21,28,29,34,37].…”

Section: Discussionmentioning

confidence: 99%

The temperature of caffeine administered during pregnancy and foetal morphometric parameters

et al. 2014

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Background: Caffeine is one of the most frequently ingested (at various tempe- Materials and methods: The research was conducted on rats. The fertilised females were randomly divided into two main groups: an experimental (E) and a control group (C). The experimental groups received caffeine (30 mg/day) in 10 (E1), 25 (E2) and 45°C (E3). The females in the control group were given water at the same temperature (C 1 , C 2 and C 3 ). On the 21 st day of pregnancy, the pregnant females were killed by decapitation using a specially prepared laboratory guillotine and were assessed morphometric parameters of foetuses.Results and Conclusions: Based on this work showed that: the embryotoxic effect of caffeine was only confined to a reduction in the number of offspring; the greatest changes in the morphometric parameters occurred in foetuses whose mothers received caffeine at 10°C; in the control groups, the greatest changes were observed in foetuses whose mothers were given water at 10°C during pregnancy. (Folia Morphol 2014; 72, 3: 347-352)

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“…Animal studies have shown that high levels of caffeine consumption during gestation produce teratogenic effects resulting in malformations, including NTDs (Lee et al, 1982;Wilkinson and Pollard, 1994;Marret et al, 1997). The teratogenic effects observed in animal studies were stronger when caffeine exposure was combined with other exposures, such as low folate levels (Heid et al, 1992) and alcohol consumption (Ross and Persaud, 1989).…”

Section: Introductionmentioning

confidence: 95%