In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

Dumas, Laura; Clavreul, Solène; Durand, Jason; Hernández-Garzón, Edwin; Abdeladim, Lamiae; Barry‐Martinet, Raphaëlle; Caballero-Megido, Alicia; Beaurepaire, Emmanuel; Bonvento, Gilles; Livet, Jean; Loulier, Karine

doi:10.3791/61110

Cited by 3 publications

(3 citation statements)

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“…MAGIC Markers (MM) strategy based on Brainbow transgenes provides a wide range of markers to individualize a large number of neighboring cells. Following in utero electroporation of MM transgenes at a precise developmental stage where the combinatorial markers are inserted in the genome of dividing cortical stem cells, the persistence of color combinations over numerous rounds of cell divisions enables to track cell identity and location of sister cells during brain development [ 119 ]. This strategy showed that dividing cortical stem cells during the early stages of embryonic neurogenesis contributed to the pool of adult NSC that generated the neurons of the olfactory bulb during adult neurogenesis [ 1 ] and that cortical astrocyte development is plastic at clonal and cellular levels [ 46 ].…”

Section: Contribution Of Multicolor Strategies To the Study Of Tissue...mentioning

confidence: 99%

“…For example, terminal Schwann cells, Bergmann glia and astrocytes are labeled in several Thy1- Brainbow lines (B, G, M and Q, [ 2 ]). Singled out after electroporation of MAGIC Markers in dorsal progenitors, individual cortical astrocytes progressively increase their volume and process complexity whereas clonal expansion and proliferation stops between the first and third postnatal week [ 46 , 119 ]. Furthermore, adult neighboring astrocytes marked with membrane-targeted FP display a continuously tiling of the cortex while interdigitating in the colliculus of Thy1-Brainbow1.1 line M; CAGGS-Cre ERT2 mouse [ 2 ].…”

Section: Delving Into Brain Cytoarchitecture Using Multicolor Approachesmentioning

confidence: 99%

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Multicolor strategies for investigating clonal expansion and tissue plasticity

Dumas

Clavreul

Michon

et al. 2022

Cell. Mol. Life Sci.

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Understanding the generation of complexity in living organisms requires the use of lineage tracing tools at a multicellular scale. In this review, we describe the different multicolor strategies focusing on mouse models expressing several fluorescent reporter proteins, generated by classical (MADM, Brainbow and its multiple derivatives) or acute (StarTrack, CLoNe, MAGIC Markers, iOn, viral vectors) transgenesis. After detailing the multi-reporter genetic strategies that serve as a basis for the establishment of these multicolor mouse models, we briefly mention other animal and cellular models (zebrafish, chicken, drosophila, iPSC) that also rely on these constructs. Then, we highlight practical applications of multicolor mouse models to better understand organogenesis at single progenitor scale (clonal analyses) in the brain and briefly in several other tissues (intestine, skin, vascular, hematopoietic and immune systems). In addition, we detail the critical contribution of multicolor fate mapping strategies in apprehending the fine cellular choreography underlying tissue morphogenesis in several models with a particular focus on brain cytoarchitecture in health and diseases. Finally, we present the latest technological advances in multichannel and in-depth imaging, and automated analyses that enable to better exploit the large amount of data generated from multicolored tissues.

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Section: Contribution Of Multicolor Strategies To the Study Of Tissue...mentioning

confidence: 99%

Section: Delving Into Brain Cytoarchitecture Using Multicolor Approachesmentioning

confidence: 99%

Multicolor strategies for investigating clonal expansion and tissue plasticity

Dumas

Clavreul

Michon

et al. 2022

Cell. Mol. Life Sci.

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“…Transposons, such as piggyBac [ 88 , 89 ], Sleeping Beauty (SB) [ 90 , 91 ], and Tol2 [ 92 ], can be implemented to integrate an exogenous gene into the genome of the host, providing stable, long-term gene expression. The combination of piggyBac/Tol2 transposition and Cre/lox recombination expressed by IUE could be utilized for multicolor fate mapping in the developing brain [ 93 , 94 ]. SB with IUE has been used to induce glioma in the postnatal mouse brain [ 95 ], providing a tool for functional analysis of the candidate genes in glioma.…”

Section: Spatiotemporal Expression Control and Lineage Tracing By Iuementioning

confidence: 99%

Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders

Yang,

Shitamukai,

Yang

et al. 2023

IJMS

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The mammalian cerebral cortex undergoes a strictly regulated developmental process. Detailed in situ visualizations, imaging of these dynamic processes, and in vivo functional gene studies significantly enhance our understanding of brain development and related disorders. This review introduces basic techniques and recent advancements in in vivo electroporation for investigating the molecular mechanisms underlying cerebral diseases. In utero electroporation (IUE) is extensively used to visualize and modify these processes, including the forced expression of pathological mutants in human diseases; thus, this method can be used to establish animal disease models. The advent of advanced techniques, such as genome editing, including de novo knockout, knock-in, epigenetic editing, and spatiotemporal gene regulation, has further expanded our list of investigative tools. These tools include the iON expression switch for the precise control of timing and copy numbers of exogenous genes and TEMPO for investigating the temporal effects of genes. We also introduce the iGONAD method, an improved genome editing via oviductal nucleic acid delivery approach, as a novel genome-editing technique that has accelerated brain development exploration. These advanced in vivo electroporation methods are expected to provide valuable insights into pathological conditions associated with human brain disorders.

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