In Utero and Exo Utero Surgery on Rodent Embryos

Ngô-Muller, Valérie; Muneoka, Ken

doi:10.1016/s0076-6879(10)76012-2

Cited by 9 publications

(4 citation statements)

References 61 publications

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“…In utero electroporation was performed as described (Ngô-Muller and Muneoka, 2010; Tabata and Nakajima, 2008) on pregnant dams carrying E13.5 embryos by injecting shNR2Bi and shNR2Bm plasmids, separately or together, for knockdown of NR2B expression (Zhou et al, 2012b) (provided by Hongbing Wang, Michigan State University, East Lansing, Michigan, USA), each at 1 µg/µl. In a second set of experiments, knockdown of KCC2 was achieved with shKCC2 plasmid (Bortone and Polleux, 2009) (provided by Franck Polleux, University of North Carolina, Chapel Hill, North Carolina, USA), at 1 µg/µl.…”

Section: Methodsmentioning

confidence: 99%

Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons

Zechel

Nakagawa

Ibáñez

2016

eLife

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Neocortical GABAergic interneuron migration and thalamo-cortical axon (TCA) pathfinding follow similar trajectories and timing, suggesting they may be interdependent. The mechanisms that regulate the radial dispersion of neocortical interneurons are incompletely understood. Here we report that disruption of TCA innervation, or TCA-derived glutamate, affected the laminar distribution of GABAergic interneurons in mouse neocortex, resulting in abnormal accumulation in deep layers of interneurons that failed to switch from tangential to radial orientation. Expression of the KCC2 cotransporter was elevated in interneurons of denervated cortex, and KCC2 deletion restored normal interneuron lamination in the absence of TCAs. Disruption of interneuron NMDA receptors or pharmacological inhibition of calpain also led to increased KCC2 expression and defective radial dispersion of interneurons. Thus, although TCAs are not required to guide the tangential migration of GABAergic interneurons, they provide crucial signals that restrict interneuron KCC2 levels, allowing coordinated neocortical invasion of TCAs and interneurons.DOI: http://dx.doi.org/10.7554/eLife.20770.001

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Section: Methodsmentioning

confidence: 99%

Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons

Zechel

Nakagawa

Ibáñez

2016

eLife

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“…The second caveat lies in the limitation of mouse embryo culture, which cannot exceed 24 hr. Application of ex utero surgery can extend the development of labeled embryos to a certain extent, but cannot completely circumvent this limitation (Serbedzija et al, ; Ngo‐Muller and Muneoka, ). Neural crest Cre transgenic mice, such as Wnt1Cre (Danielian et al, ), enable permanent labeling of neural crest derived cells in vivo.…”

Section: Discussionmentioning

confidence: 99%

Sox10ER^T2CreER^T2 mice enable tracing of distinct neural crest cell populations

Soriano

2015

Developmental Dynamics

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Background Neural crest cells play an important role in craniofacial morphogenesis and many other developmental processes. The formation of neural crest cells (NCCs) in vivo is a highly dynamic process and remains to be fully understood. Results To investigate the spatiotemporal patterning of NCCs in vivo, we have generated Sox10ERT2CreERT2 (SECE) mice, a transgenic line driving inducible Cre expression in NCCs. Inducing Cre activity at different stages triggered reporter expression in distinct NCC populations in SECE; R26R mice. By optimizing the timing and dosage of tamoxifen administration, we controlled Cre expression specifically in cranial NCCs. Using this approach, we demonstrate an important role for PDGFRα in cranial NCCs mitosis within the mandibular processes. Further reducing Cre activity within the cranial NCCs of SECE; R26R embryos revealed that SECE labels preferentially progenitors of medial nasal process (MNP) rather than the lateral nasal process (LNP), before their formation from the frontonasal prominence (FNP). Conclusions Our results indicate that NCCs are formed sequentially from rostral to caudal regions along the neural tube. These findings also suggest that NCCs within the FNP become specified regionally and genetically before they divide into MNP and LNP.

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“…Uterine in vivo gene transfer has been demonstrated in mice (Charnock-Jones et al, 1997;Kimura et al, 2005;Rodde et al, 2008) and rabbits (Laurema et al, 2007). However, accurate access to the lumen of uterus in small animals requires invasive surgical procedures (Ngô-Muller & Muneoka, 2010). As with ovaries and oviducts, transrectal ultrasonography could improve vector cellular uptake via sonoporation (Maruyama et al, 2004).…”

Section: In Vivo Gene Delivery To the Uterusmentioning

confidence: 99%

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

Velazquez¹,

Kues²

2011

Biomedical Engineering, Trends, Research and Technologies

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In Utero and Exo Utero Surgery on Rodent Embryos

Cited by 9 publications

References 61 publications

Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons

Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons

Sox10ER^T2CreER^T2 mice enable tracing of distinct neural crest cell populations

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

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In Utero and Exo Utero Surgery on Rodent Embryos

Cited by 9 publications

References 61 publications

Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons

Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons

Sox10ERT2CreERT2 mice enable tracing of distinct neural crest cell populations

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

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Product

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Sox10ER^T2CreER^T2 mice enable tracing of distinct neural crest cell populations