In Tuber Biocontrol of Potato Late Blight by a Collection of Phenazine-1-Carboxylic Acid-Producing Pseudomonas spp.

Léger, Geneviève; Novinscak, Amy; Biessy, Adrien; Lamarre, Simon G.; Filion, Martin

doi:10.3390/microorganisms9122525

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…strains producing phenazine-1-carboxylic acid. [ 22 ]. Metabolites extracted from Myxococcus strains demonstrated activity against PLB [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

A New Biocontrol Tool to Fight Potato Late Blight Based on Willaertia magna C2c Maky Lysate

Troussieux¹,

Gilgen²,

Souche³

2022

Plants

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Potato late blight (PLB) is one of the most destructive disease affecting potatoes. Late blight control relies almost exclusively on the use of chemical pesticides, including copper products, which are efficient but controversial due to their environmental toxicity. Societal pressure and the quest for more sustainable agriculture reinforce the need for natural plant protection products. To respond to this demand, we tested the lysate of the amoeba Willaertia magna C2c Maky on PLB. This active substance exhibits plant protection properties against grape downy mildew thanks to a dual mode of action (plant elicitor and antifungal direct effect). We hypothesized that this active substance might also have an effect against other diseases caused by oomycetes on other crops, such as potato. In vitro, in planta, and in-field studies were conducted. The collected data demonstrate that the lysate of the amoeba Willaertia magna C2c Maky is able to elicit potato defenses, and direct fungicidal activity against Phytophtora infestans was observed. Proof of efficacy was first obtained in greenhouse, with up to 80% disease reduction, and confirmed in field trials. Formulated products provided up to 77% protection in field in the case of low infestation (28%) and up to 49% protection when the untreated plants were 100% destroyed. Willaertia magna C2c Maky was also able to significantly increase yield by up to 30% in field trials.

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“…strains producing phenazine-1-carboxylic acid. [ 22 ]. Metabolites extracted from Myxococcus strains demonstrated activity against PLB [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

A New Biocontrol Tool to Fight Potato Late Blight Based on Willaertia magna C2c Maky Lysate

Troussieux¹,

Gilgen²,

Souche³

2022

Plants

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“…The precipitate was incubated again at 4 • C for 4 h. After removal, the supernatant was retained by centrifuging at 8000 rpm for 20 min. The antifungal activity of the supernatant and acid precipitate was determined using the Oxford cup method [19].…”

Section: Effects Of Bactericidal Extracts On Pathogen Growth and Meta...mentioning

confidence: 99%

Screening and Analysis of Antifungal Strains Bacillus subtilis JF-4 and B. amylum JF-5 for the Biological Control of Fusarium Wilt of Banana

Duan,

Pang,

Yin

et al. 2023

JoF

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Purpose: This study aimed to identify the antagonistic bacteria from the rhizosphere of healthy bananas that can effectively suppress the Fusarium wilt of banana, and to further investigate the inhibitory mechanism. Method: The primary and secondary screening techniques were implemented using the double-plate and fermentation antagonism methods. The strain was identified based on physiological and biochemical tests, 16S rRNA gene sequencing, and specific gene amplification. The effects of crude extract on the protein content, lipid peroxidation, and pectinase activity of mycelia were determined from the identified isolates. Results: Two antagonistic bacteria, JF-4 and JF-5, were screened and initially identified as Bacillus subtilis (GenBank: OR125631) and B. amylum (GenBank: OR125632). The greenhouse experiment showed that the biological control efficiency of the two antagonists against the Fusarium wilt of banana was 48.3% and 40.3%, respectively. The catalase content produced by lipid peroxidation increased significantly after treatment with the crude extracts of JF-4 and JF-5 at concentrations of 0.69 μmol/L and 0.59 μmol/L, respectively. The protein and ergosterol content and pectinase activity decreased significantly. The two antagonistic bacteria might inhibit the growth of pathogens by enhancing lipid peroxidation and decreasing the synthesis of cell metabolites. Twenty compounds were identified by gas chromatography–mass spectrometry (GC-MS). B. subtilis JF-4 was further sequenced and assembled to obtain a complete circular chromosome genome of 681,804,824 bp. The genome consisted of a 4,310,825-bp-long scaffold. Conclusion: The findings of this study may help elucidate the mechanism behind this biocontrol isolate.

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“…Léger et al 57 . compared the biocontrol activities of 23 PCA‐producing Pseudomonas strains against Phytophthora infestans , finding that Pseudomonas orientalis (R4‐35‐08, R2‐66‐08W and L1‐3‐08), Pseudomonas yamanorum (LBUM636), Pseudomonas aridus (R4‐34‐07), Pseudomonas synxantha (2–79) and Pseudomonas sp.…”

Section: Bacteria As Biocontrol Agents and Their Modes Of Actionmentioning

confidence: 99%

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

Wang

Long

2023

Pest Management Science

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Phytophthora infestans causes light blight on potatoes and tomatoes, which has a significant economic impact on agriculture. The management of late blight has been largely dependent on the application of synthetic fungicides, which is not ultimate solution for sustainable agriculture and environmental safety. Biocontrol strategies are expected to be alternative methods to the conventional chemicals in controlling plant diseases in the integrated pest management (IPM) programs. Well‐studied biocontrol agents against P. infestans include fungi, oomycetes, bacteria, and compounds produced by these antagonists, in addition to certain bioactive metabolites produced by plants. Laboratory and greenhouse experiments suggest a potential for using biocontrol in practical late blight disease management. However, the transition of biocontrol to field applications is problematic for the moment, due to low and variable efficacies. In this review, we provide a comprehensive summary on these biocontrol strategies and the underlying corresponding mechanisms. To give a more intuitive understanding of the promising biocontrol agents against P. infestans in agricultural systems, we discuss the utilizations, modes of action and future potentials of these antagonists based on their taxonomic classifications. To achieve a goal of best possible results produced by biocontrol agents, it is suggested to work on field trials, strain modifications, formulations, regulations, and optimizations of application. Combined biocontrol agents having different modes of action or biological adaptation traits may be used to strengthen the biocontrol efficacy. More importantly, biological control agents should be applied in the coordination of other existing and forthcoming methods in the IPM programs.This article is protected by copyright. All rights reserved.

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In Tuber Biocontrol of Potato Late Blight by a Collection of Phenazine-1-Carboxylic Acid-Producing Pseudomonas spp.

Cited by 9 publications

References 44 publications

A New Biocontrol Tool to Fight Potato Late Blight Based on Willaertia magna C2c Maky Lysate

A New Biocontrol Tool to Fight Potato Late Blight Based on Willaertia magna C2c Maky Lysate

Screening and Analysis of Antifungal Strains Bacillus subtilis JF-4 and B. amylum JF-5 for the Biological Control of Fusarium Wilt of Banana

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

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