In the ventral tegmental area picrotoxin blocks FGIN 1-27-induced increases in sexual behavior of rats and hamsters

Petralia, Sandra M.; Frye, Cheryl A.

doi:10.1007/s00213-004-2001-9

Cited by 21 publications

(15 citation statements)

References 43 publications

(44 reference statements)

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“…In support, GBRs have been localized to nondopaminergic cells in the VTA using autoradiography (Churchill et al 1992). These GBRs are important components of P's actions for lordosis because antagonism of GBRs in the VTA, with picrotoxin or bicuculline, reduces P-facilitated lordosis of rats and hamsters (Frye 2001a−c;Petralia and Frye 2005;Frye et al 1993). Although GBRs are ionotropic receptors, in vitro studies using hypothalamic sections show that blocking G-protein activity reduces progestin-mediated increases in the amount of time that the chloride channel remains open (Fancsik et al 2000).…”

Section: Discussionmentioning

confidence: 99%

In the ventral tegmental area, G-proteins mediate progesterone’s actions at dopamine type 1 receptors for lordosis of rats and hamsters

Petralia

Frye

2006

Psychopharmacology

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Section: Discussionmentioning

confidence: 99%

In the ventral tegmental area, G-proteins mediate progesterone’s actions at dopamine type 1 receptors for lordosis of rats and hamsters

Petralia

Frye

2006

Psychopharmacology

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“…Progesterone from peripheral origin or central biosynthesis is metabolized to dihydroprogesterone (DHP) by 5a-reductase and, subsequently, into 5a-pregnan3a-ol-20-one (3a,5a-THP) by 3a-hydroxysteroid oxidoreductase (3aHSOR) (Rupprecht, 2003). 3a,5a-THP facilitates female sexual behaviour via a mechanism involving the receptors dopamine type 1 (D1, stimulatory), dopamine type 2 (D2, inhibitory) (Frye et al, 2004) and g-aminobutyric acid (GABAA)/benzodiazepine receptor complex (GBR, stimulatory) (Petralia and Frye, 2005). A key role is assumed for the mitochondrial benzodiazepine receptor (MBR)-mediated increase in neurosteroidogenesis, including that of 3a,5a-THP (Petralia and Frye, 2005).…”

Section: Genomic Regulation Of Oestrus (Behaviour) From the Brainmentioning

confidence: 99%

“…3a,5a-THP facilitates female sexual behaviour via a mechanism involving the receptors dopamine type 1 (D1, stimulatory), dopamine type 2 (D2, inhibitory) (Frye et al, 2004) and g-aminobutyric acid (GABAA)/benzodiazepine receptor complex (GBR, stimulatory) (Petralia and Frye, 2005). A key role is assumed for the mitochondrial benzodiazepine receptor (MBR)-mediated increase in neurosteroidogenesis, including that of 3a,5a-THP (Petralia and Frye, 2005).…”

Section: Genomic Regulation Of Oestrus (Behaviour) From the Brainmentioning

confidence: 99%

Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities

Beerda

Wyszyńska-Koko

Pas

et al. 2008

Animal

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Subfertility has negative effects for dairy farm profitability, animal welfare and sustainability of animal production. Increasing herd sizes and economic pressures restrict the amount of time that farmers can spend on counteractive management. Genetic improvement will become increasingly important to restore reproductive performance. Complementary to traditional breeding value estimation procedures, genomic selection based on genome-wide information will become more widely applied. Functional genomics, including transcriptomics (gene expression profiling), produces the information to understand the consequences of selection as it helps to unravel physiological mechanisms underlying female fertility traits. Insight into the latter is needed to develop new effective management strategies to combat subfertility. Here, the importance of functional genomics for dairy cow reproduction so far and in the near future is evaluated. Recent gene profiling studies in the field of dairy cow fertility are reviewed and new data are presented on genes that are expressed in the brains of dairy cows and that are involved in dairy cow oestrus (behaviour). Fast-developing new research areas in the field of functional genomics, such as epigenetics, RNA interference, variable copy numbers and nutrigenomics, are discussed including their promising future value for dairy cow fertility.

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“…In the VTA, inhibiting GABA synthesis reduces, and blocking GABA degradation, enhances P-facilitated lordosis of hamsters or rats [13, 14]. Also, in the VTA, inhibiting GABA A /benzodiazepine receptor complexes (GBRs) with bicuculline or picrotoxin decreases, and activating GBRs with muscimol increases, P-facilitated lordosis of hamsters and rats [5, 7, 8, 13,15,16,17]. Thus, progestin-facilitated lordosis is reliably enhanced by activation of D 1 or GBRs in the VTA, suggesting that progestins’ actions for lordosis may include agonist-like actions at these receptors.…”

Section: Introductionmentioning

confidence: 99%

In the Ventral Tegmental Area, Progestins’ Membrane-Mediated Actions for Lordosis of Hamsters and Rats Involve Protein Kinase A

Petralia

Walf²,

Frye³

2006

Neuroendocrinology

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Progestin-facilitated lordosis of hamsters and rats is enhanced by activation of dopamine type 1 (D₁) or GABA_A/benzodiazepine receptor complexes (GBRs) in the ventral tegmental area (VTA) and these effects involve G-proteins and second messengers, such as adenosine 3′,5′-monophosphate (cAMP). We examined whether D₁- and/or GBR-mediated increases in progestin-facilitated lordosis of female hamsters and rats involve the cAMP-dependent protein kinase, protein kinase A (PKA), in the VTA. In experiment 1, ovariectomized hamsters, primed with estradiol (E₂; 10 µg at h 0) + progesterone (P; 100 µg at h 45), were first pre-tested for lordosis and motor behavior (h 48) and then infused with the PKA inhibitor, Rp-cAMP (100 ng/side), or vehicle. Thirty minutes later, hamsters were retested and then received infusions of the D₁ agonist, SKF38393 (100 ng/side), the GBR agonist, muscimol (100 ng/side), or vehicle to the VTA. Hamsters were post-tested for lordosis and motor behavior 30 min later. In Experiment 2, ovariectomized rats, primed with E₂ (10 µg at h 0), were first pre-tested for lordosis and then infused with Rp-cAMP (100 ng/side) or vehicle to the VTA at h 44. Immediately after testing, rats received infusions of SKF38393 (100 ng/side), muscimol (100 ng/side), or vehicle and were retested for lordosis. Rats were then infused with the neurosteroid, 5α-pregnan-3α-ol-20-one (3α,5α-THP; 100 or 200 ng/side), or β-cyclodextrin vehicle and were post-tested for lordosis and motor behavior 10 and 60 min later. The enhancing effects of progestins or progestins plus D₁ or GBR activation on lordosis of E₂-primed hamsters and rats were blocked by the PKA inhibitor, Rp-cAMP. Thus, in the VTA, progestins’ membrane actions involving D₁ or GBRs are mediated, in part, by PKA.

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In the ventral tegmental area picrotoxin blocks FGIN 1-27-induced increases in sexual behavior of rats and hamsters

Abstract: In the VTA, GBRs are required for MBR-enhanced sexual behavior of EB-primed rats and EB- and progesterone-primed hamsters.

Cited by 21 publications

References 43 publications

In the ventral tegmental area, G-proteins mediate progesterone’s actions at dopamine type 1 receptors for lordosis of rats and hamsters

In the ventral tegmental area, G-proteins mediate progesterone’s actions at dopamine type 1 receptors for lordosis of rats and hamsters

Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities

In the Ventral Tegmental Area, Progestins’ Membrane-Mediated Actions for Lordosis of Hamsters and Rats Involve Protein Kinase A

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