In‐Tandem Column Arrangement for Rapid Purification of IgG using a Blue Avid Gel P and an Aza‐Arenophilic Affinity Gel

Abstract

An in-tandem study comprising two affinity chromatographic columns, Blue Avidgel P (for the selective removal of albumin) and an aza-arenophilic gel (Gel A) containing a nitrogen capping nucleophile (for the selective removal of IgG), was conducted to obtain high purity IgG from serum. The results demonstrated that highly pure IgG (.97%) can be obtained from the phenethylamine capped aza-arenophilic gel column after the serum was passed through the Blue Avidgel P column in contrast to the 38% IgG purity obtained when the serum was passed through a Gel A column alone under similar conditions. Further studies show that IgG selectivity is not dependent on the nature of the support material used in the synthesis of these aza-arenophilic gels since the synthesis of aza-arenophilic gels using either Sepharose or Fractogel yield similar results.