In situ tracking of microbeads for the detection of biofilm formation

Abstract

In this study, we utilize the free motion of beads incorporated in bacterial suspension to investigate the behavior of the medium surrounding the beads during biofilm formation. The use of imaging techniques such as digital image correlation enables tracking of the movement of beads, which serve as markers in the processed images. This method is applied to detect and characterize biofilm formation. The main originality of this study lies in characterizing the evolution of the typology of bead movements during biofilm formation. The aim is to identify bead behaviors that represent the start of biofilm formation. By observing inert bead movements introduced into the bacterial environment, changes in trajectory typologies are detected and appear to be related to sessile bacterial activity, bacterial hindrance, and adhesion or formation of extracellular material. We use our approach to discriminate between the presence or absence of antibiotics mixed with bacteria and to assess their effectiveness. The results highlight the potential of our approach as nondestructive tracking of biofilm dynamics over time based on optical microscope images.

Promising strategies to control persistent enemies: Some new technologies to combat biofilm in the food industry—A review

“…The essential of the attachment stage of biofilm includes the fast connection of organisms to the surface and the expansion of cells. Phenomena, such as sedimentation, hydrodynamics, and Brownian motion, are involved in settling bacterial cells on a surface (Boudarel et al, 2021;Matysik & Kline, 2019). By contrast, the substrate attachment is conditioned by attractive forces, such as Lifshitz-Van der Waals, acid-base binding, hydrophobic, and electrostatic doublelayer interactions (Khelissa et al, 2017).…”

Molecular and Colloidal Transport in Bacterial Cellulose Hydrogels

“…In random fiber networks, mesh size, alignment, and heterogeneity can lead to strong variations in microparticle transport rates − and localization. , Particle dynamics are coupled to any spatial heterogeneity of the matrix, for example, affecting drug transport in cancer tissue , as well as the movement of soft particle medical delivery vehicles. − Complex biological network structures can vary significantly, but diffusive transport can be measured in the matrix via molecular or colloidal tracer studies. − Diffusive transport in EPS biofilm networks has been studied to measure how rheology, , diffusive permeability, , and restructuring modulate transport in the matrix, , but cellulose biofilms have not been characterized in the same way. In this study, we use optical microscopy to track molecular and particulate tracers to characterize mobility within anisotropic, hierarchically structured bacterial cellulose films and show the diversity of natural structures and properties found there.…”

“…30−32 Complex biological network structures can vary significantly, 33 but diffusive transport can be measured in the matrix via molecular or colloidal tracer studies. 34−38 Diffusive transport in EPS biofilm networks has been studied to measure how rheology, 39,40 diffusive permeability, 41,42 and restructuring modulate transport in the matrix, 43,44 but cellulose biofilms have not been characterized in the same way. In this study, we use optical microscopy to track molecular and particulate 45 tracers to characterize mobility within anisotropic, hierarchically structured bacterial cellulose films and show the diversity of natural structures and properties found there.…”

Molecular and colloidal transport in bacterial cellulose biofilms

“…Complex biological network structures can vary significantly 31 , but diffusive transport can be measured in the matrix via molecular or colloidal tracer studies [32][33][34][35][36] . Diffusive transport in EPS biofilm networks has been studied to measure how rheology 37,38 , diffusive permeability 39,40 , and restructuring modulate transport in the matrix 41,42 , but cellulose biofilms have not been characterized in the same way. In this study, we use optical microscopy to track molecular and particulate 43 tracers to characterize mobility within anisotropic, hierarchically structured bacterial cellulose films and show the diversity of natural structures and properties found there.…”