In situ synthesis of multidentate PEGylated chitosan modified gold nanoparticles with good stability and biocompatibility

Abstract

To realize desirable functions in the rather complex biological systems, a suitable surface coating is greatly in need for gold nanoparticles, which plays an important role in their colloidal stability and biocompatibility. In this work, a novel multidentate PEGylated chitosan derivative was synthesized by conjugating PEG and dithiolane lipoic acid (LA) to the chitosan backbone. Under the reduction conditions, gold nanoparticles (AuNPs) could be in-situ formed by the electrostatic interaction between amino groups of the chitosan derivative and gold chloride ions (AuCl 4 -), with surface covalently coated by the multidentate PEGylated chitosan via the disulfide bond from LA. After the surface modification, such AuNPs exhibited remarkable colloidal stabilities under extreme conditions, including high salt conditions and complex biological media containing serum. Moreover, the multidentate PEGylated chitosan also provided AuNPs with good biocompatibility in low cytotoxicity, resistance of protein adsorption and anti-phagocytosis by RAW 264.7 cells. This kind of AuNPs was supposed to be promising platform for applications in nanomedicine.

Effects of PEG surface density and chain length on the pharmacokinetics and biodistribution of methotrexate-loaded chitosan nanoparticles

“…Extensive research has been carried out on PEGylation of biomolecules to improve their water solubility and prolong blood circulation time to escape the phagocytosis of macrophages and avoid RES clearance. [9][10][11] Several strategies have reported the chemical conjugation of mPEG with CS through C2-amino group or C6-hydroxyl group. [12][13][14] The PEG-g-CS copolymers (with different molecular weight [MW] or substitution degree of the mPEG graft) were used in a different application for a drug delivery system.…”

PEGylated chitosan protected silver nanoparticles as water-borne coating for leather with antibacterial property

“…The amino substitution degree (SD) of PEG-g-CS defined as the number of PEG group per 100 amino groups of CS was determined by TNBS method [36]. In this work, the PEG-g-CS that we used possessed a SD of 7.5 ± 2%.…”

“…Dead bacteria cells could also be easily washed from the PEGylated quaternized chitosan coating reported by Chan-Park's group, and the coating preserved its antimicrobial activity even after the fourth challenge with S. aureus [13]. Moreover, PEGylation of chitosan has also been shown to decrease cytotoxicity [34][35][36], which is suitable to construct hygeian leather coating.…”

Denatured proteins as a novel template for the synthesis of well-defined, ultra-stable and water-soluble metal nanostructures for catalytic applications

“…Recently, the environmentally friendly synthesis and stabilization of metal nanoparticles using biopolymers have become a trend [15][16][17][18][19]. For instance, chitosan [20][21][22], cellulose [23], gelatin [24], bovine serum albumin [25,26], and collagen that can be extracted from leather products [27], have been reported as templates for the synthesis of gold nanoparticles at room temperature. In the past decade, our group has developed a facile synthesis route to unfold native proteins including human serum albumin (HSA) and lysozyme to well-defined linear biopolymers [28,29].…”