In situ synthesis of a monolithic material with multi-sized pores and its chromatographic properties for the separation of intact proteins from human plasma

Lan, Dandan; Bai, Ligai; Pang, Xiaomin; Liu, Haiyan; Yan, Hongyuan; Guo, Hongwei

doi:10.1016/j.talanta.2018.10.054

Cited by 19 publications

(12 citation statements)

References 36 publications

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“…The composite POC-doped monolithic adsorbent was prepared in a stainless-steel column (4.6 mm id × 50 mm) using DAIP as the monomer, EDMA as the crosslinker, n-propyl alcohol, 1-dodecanol, and N,N-dimethylformamide as triple porogens, and azobisisobutyronitrile as the initiator with added POC. The preparation processes were similar to our previous work [32], and the synthetic scheme is shown in Figure 1A. Table 1 lists the representative conditions of adsorbents A1-A6 for the optimization procedure.…”

Section: Fabrication Of Composite Poc-doped Monolithic Adsorbentsmentioning

confidence: 99%

Extraction and determination of tussilagone from Farfarae Flos with online solid‐phase extraction–high‐performance liquid chromatography using a homemade monolithic cartridge doped with porous organic cage material

Wang

Liu

Sun

et al. 2021

J of Separation Science

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A solid-phase extraction cartridge was fabricated using diallyl isophthalate as the monomer with the addition of porous organic cage material via in situ free-radical polymerization in a stainless-steel column. The resulting monolithic adsorbent exhibited a relatively uniform porous structure, a high specific surface area of 113.98 m 2 /g, and multiple functional chemical groups according to the characterization results. An online solid-phase extraction-high-performance liquid chromatography procedure was fabricated to extract and determine tussilagone from Farfarae Flos. The results show that the complex sample matrices can be removed in the solid-phase extraction procedure. Simultaneously, tussilagone can remain, which can be subsequently switched to an octadecylsilane bonded analytical column. The methodological validation showed that the correlation coefficient was 0.9999 with a linear range of 0.6-200.0 µg/mL, the limit of detection was 0.2 µg/mL, the limit of quantification was 0.6 µg/mL, accuracy was 100.3-100.6%, and relative standard deviation of precision was ≤1.9%. The present monolithic cartridge exhibits good reusability of not more than 100 times. The real sample of Farfarae Flos was determined with a tussilagone content of 0.74 mg/g.

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Section: Fabrication Of Composite Poc-doped Monolithic Adsorbentsmentioning

confidence: 99%

Extraction and determination of tussilagone from Farfarae Flos with online solid‐phase extraction–high‐performance liquid chromatography using a homemade monolithic cartridge doped with porous organic cage material

Wang

Liu

Sun

et al. 2021

J of Separation Science

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“…[7] The adsorption materials commonly used in current research involve MOFs, [8] silica-based materials, [9,10] carbon-based materials, [11,12] magnetic composite [13][14][15] and macroporous adsorption resin (MAR), COFs, [16,17] molecularly imprinted polymers (MIPs), [18][19][20][21][22][23][24] and other organic polymers. [25][26][27][28][29] Organic polymer adsorbents have become universal adsorbent materials with varieties of attractive merits, for example adjustable monomer components, relatively low-cost, structure diversity, and facile functionalization. As we all know, the structure and functional group of organic polymer adsorbent are the keys to measuring the adsorption performance.…”

Section: Doi: 101002/macp202100145mentioning

confidence: 99%

Preparation of P(EGDMA‐co‐VPBA) Adsorbent and Its Application in the Separation of Steviol Glycosides

Chen

Wang

et al. 2021

Macro Chemistry & Physics

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The poly (ethylene glycol dimethacrylate-co-vinylphenylboronic acid) P(EGDMA-co-VPBA) adsorbents are prepared via reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization with 4-vinylphenylboronic acid (VPBA) as the functional monomer, and used for selective adsorption of steviol glycosides (SGs). The boron content and pore structure of the P(EGDMA-co-VPBA) adsorbent are adjusted by changing the polymerization conditions. The adsorption results show that the pore structure of the P(EGDMA-co-VPBA) affects the adsorption capacity to SGs, the presence of boric acid functional groups shows favorable adsorption selectivity for stevioside (STV), and the adsorption selectivity is proportional to the content of boric acid in P(EGDMA-co-VPBA) adsorbents. The adsorption performance of P(EGDMA-co-VPBA) toward SGs is the best when the ratio of monomer to cross-linker is 1:50 and the solvent is acetonitrile. In the SG solution (6.0 mg mL −1 , C STV /C RA = 1:1), the adsorption capacities and adsorption selectivity ( STV/RA ) of P(EGDMA-co-VPBA) to SGs are 113.20 mg g -1 and 4.99, respectively. The adsorption kinetics and isotherm data obey pseudo-second-order kinetic model and Langmuir isotherm model, respectively. The boric acid copolymers with VPBA as the monomer prepared by the one-step method can become a promising material for efficient separation and purification of SGs.

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“…Lan et al used in situ radical polymerization to produce a novel type of phenoxyacetate-based monolithic material with multi-sized pores. (100) The columns showed excellent selectivity for proteins due to its multiple interaction sites with proteins, such as π-π stacking interactions, hydrophobic interactions, and intermolecular interactions.…”

Section: Polymer Monolithsmentioning

confidence: 99%

“…The results obtained suggest that the freezing of the solvent crystals occurs in the direction of the temperature gradient from the surface of the reactor to its center rather than in the freezing direction. Lan et al used in situ radical polymerization to produce a novel type of phenoxyacetate-based monolithic material with multisized pores . The columns showed excellent selectivity for proteins because of its multiple interaction sites with proteins, such as π–π stacking interactions, hydrophobic interactions, and intermolecular interactions.…”

Section: Monolithic Columnsmentioning

confidence: 99%

Advances and Innovations in Liquid Chromatography Stationary Phase Supports

Broeckhoven

Desmet

2020

Anal. Chem.

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In situ synthesis of a monolithic material with multi-sized pores and its chromatographic properties for the separation of intact proteins from human plasma

Cited by 19 publications

References 36 publications

Extraction and determination of tussilagone from Farfarae Flos with online solid‐phase extraction–high‐performance liquid chromatography using a homemade monolithic cartridge doped with porous organic cage material

Extraction and determination of tussilagone from Farfarae Flos with online solid‐phase extraction–high‐performance liquid chromatography using a homemade monolithic cartridge doped with porous organic cage material

Preparation of P(EGDMA‐co‐VPBA) Adsorbent and Its Application in the Separation of Steviol Glycosides

Advances and Innovations in Liquid Chromatography Stationary Phase Supports

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