In situ single step detection of exosome microRNA using molecular beacon

Lee, Ji Hye; Kim, Jeong Ah; Kwon, Minhae; Kang, Ji Yoon; Rhee, Won Jong

doi:10.1016/j.biomaterials.2015.03.014

Cited by 136 publications

(103 citation statements)

References 45 publications

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“…Because of the stem and loop structure, we can obtain a strong fluorescence signal from an MB only in the presence of its target (Nitin et al 2009;Rhee and Bao 2010;Rhee et al 2008;Santangelo and Bao 2007). In our report, an MB was shown to be capable of penetrating exosomes and hybridizing with its target miRNA (Lee et al 2015). As a result, the method is simpler, more sensitive, and faster than any other method developed for the detection of exosomal miRNAs, even in the presence of human serum.…”

Section: Introductionmentioning

confidence: 62%

“…This makes the PCR-based method an inefficient method for disease diagnosis. In situ single-step detection of exosomal miRNAs using MBs was successfully shown to overcome many hurdles of the PCR-based method in exosomal miRNA detection (Lee et al 2015). Within 1 h, target exosomal miRNA can be detected by a single step using a specific MB in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…Differences in intra-exosomal MB concentrations may cause different hybridization kinetics with their target miRNAs. To overcome this issue in delivery, exosome membrane permeabilization with streptolysin O (SLO) can be used (Lee et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…These processes are timeconsuming, laborious, and expensive. We have recently reported in situ single-step detection of specific miRNA in intact exosomes using a nano-sized probe, or molecular beacon (MB) (Lee et al 2015). An MB is a dual-labeled oligonucleotide hairpin probe with a fluorophore and a quencher at each end Tyagi and Alsmadi 2004).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous and multiplexed detection of exosome microRNAs using molecular beacons

Lee

Kim

Jeong

et al. 2016

Biosensors and Bioelectronics

Self Cite

132

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Section: Introductionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous and multiplexed detection of exosome microRNAs using molecular beacons

Lee

Kim

Jeong

et al. 2016

Biosensors and Bioelectronics

Self Cite

132

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“…[62][63][64] Moreover, novel probe-based technologies are emerging and may allow quantitative measures that are much faster and easier to use in a clinical context. [65][66][67] Third, we need to determine normal values in a wide population of healthy individuals in order to identify inter-individual variability with regards to gender, age, ethnicity, or training status.…”

Section: Considerations For Clinical Applicationmentioning

confidence: 99%

Circulating myomiRs: a new class of biomarkers to monitor skeletal muscle in physiology and medicine

Siracusa¹,

Koulmann

Banzet

2017

J cachexia sarcopenia muscle

100

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MicroRNAs (miRNA) are small non‐coding RNAs that target mRNAs and are consequently involved in the post‐transcriptional regulation of gene expression. Some miRNAs are ubiquitously expressed in tissue, while others are tissue‐specific or tissue‐enriched. miRNAs can be released by cells and are found in various biofluids, including serum and plasma. Thus, measuring miRNAs in the circulation may provide information on the originating tissue or cells. MyomiRs are described as striated muscle‐specific or muscle‐enriched miRNAs. Their circulating levels can be measured and have been proposed to be new biomarkers of physiological and pathological muscle processes. The aims of this review are to summarize the current knowledge of circulating myomiRs, to identify the types of information they can provide about skeletal muscle, and to determine how to apply that information in the fields of research and medicine.

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Recent Progress in Isolation and Detection of Extracellular Vesicles for Cancer Diagnostics

Wang

Luo

Wang

2018

Adv Healthcare Materials

116

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Extracellular vesicles (EVs) are emerging as one of the many new and promising biomarkers for liquid biopsy of cancer due to their loading capability of some specific proteins and nucleic acids that are closely associated with cancer states. As such, the isolation and detection of cancer-derived EVs offer important information in noninvasive diagnosis of early-stage cancer and real-time monitoring of cancer development. In light of the importance of EVs, over the last decade, researchers have made remarkable innovations to advance the development of EV isolation and detection methods by taking advantage of microfluidics, biomolecule probes, nanomaterials, surface plasmon, optics, and so on. This review introduces the basic properties of EVs and common cancer-derived EV ingredients, and provides a comprehensive overview of EV isolation and detection strategies, with emphasis on liquid biopsies of EVs for cancer diagnostics.

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In situ single step detection of exosome microRNA using molecular beacon

Cited by 136 publications

References 45 publications

Simultaneous and multiplexed detection of exosome microRNAs using molecular beacons

Simultaneous and multiplexed detection of exosome microRNAs using molecular beacons

Circulating myomiRs: a new class of biomarkers to monitor skeletal muscle in physiology and medicine

Recent Progress in Isolation and Detection of Extracellular Vesicles for Cancer Diagnostics

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