In situ remineralizaiton of partially demineralized human dentine mediated by a biomimetic non-collagen peptide

Wang, Qiao; Wang, X. M.; Tian, Lili; Cheng, Zhiqiang; Cui, Fuzhai

doi:10.1039/c1sm05018d

Cited by 57 publications

(63 citation statements)

References 35 publications

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“…This specific alignment of minerals within collagen fibrils was regulated by TPP, a templating analog of NCPs . Highly negatively charged TPP accumulates within positively charged gap zones of collagen fibrils through electrostatic attraction, which further attracts fluidic PAA‐ACP nanoprecursors to enter the gap zone of collagen fibrils via electrostatic attraction . Thus, TPP functions as an ionic bridge between collagen fibrils and PAA‐ACP nanoprecursors due to the high affinity of TPP to collagen and calcium .…”

Section: Resultsmentioning

confidence: 99%

“…Thus, TPP functions as an ionic bridge between collagen fibrils and PAA‐ACP nanoprecursors due to the high affinity of TPP to collagen and calcium . A large fraction of the charged functional groups in the gap zone recruit and bind with calcium and phosphate ions from the PAA‐ACP nanoprecursors to precipitate electron‐dense minerals within the gap zone, which results in the observed D‐banding of collagen fibrils . In extrafibrillar mineralized collagen fibrils, D‐banding is not observed.…”

Section: Resultsmentioning

confidence: 99%

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Fabrication of intrafibrillar and extrafibrillar mineralized collagen/apatite scaffolds with a hierarchical structure

Zilm

Wei

2016

J Biomedical Materials Res

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A biomimetic collagen-apatite (Col-Ap) scaffold resembling the composition and structure of natural bone from the nanoscale to the macroscale has been successfully prepared for bone tissue engineering. We have developed a bottom-up approach to fabricate hierarchically biomimetic Col-Ap scaffolds with both intrafibrillar and extrafibrillar mineralization. To achieve intrafibrillar mineralization, polyacrylic acid (PAA) was used as a sequestrating analog of noncollagenous proteins (NCPs) to form a fluidic amorphous calcium phosphate (ACP) nanoprecursor through attraction of calcium and phosphate ions. Sodium tripolyphosphate was used as a templating analog to regulate orderly deposition of apatite within collagen fibrils. Both X-ray diffraction and Fourier transform infrared spectroscopy suggest that the mineral phase was apatite. Field emission scanning electron microscopy, transmission electron microscopy, and selected area electron diffraction confirmed that hierarchical collagen-Ap scaffolds were produced with both intrafibrillar and extrafibrillar mineralization. Biomimetic Col-Ap scaffolds with both intrafibrillar and extrafibrillar mineralization (IE-Col-Ap) were compared with Col-Ap scaffolds with extrafibrillar mineralization only (E-Col-Ap) as well as pure collagen scaffolds in vitro for cellular proliferation using MC3T3-E1 cells. Pure collagen scaffolds had the highest rate of proliferation, while there was no statistically significant difference between IE-Col-Ap and E-Col-Ap scaffolds. Thus, the bottom-up biomimetic fabrication approach has rendered a group of promising Col-Ap scaffolds, which bear high resemblance to natural bone in terms of composition and structure.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Fabrication of intrafibrillar and extrafibrillar mineralized collagen/apatite scaffolds with a hierarchical structure

Zilm

Wei

2016

J Biomedical Materials Res

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“…33,45,46 A large fraction of charged amino acid side chains in the gap zone helps to recruit and bind calcium and phosphate ions, and the resulting calcium-phosphate prenucleation cluster become crystallized HA with time. 8,34,46 Thus the gap zone of collagen fibrils serves as the entering (as inferred from the insert image in Figure 3D) and mineralization sites, which forms the D-banding of collagen fibrils (Figure 7). This is in agreement with the finding by 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Figure 1A and Figure 2A).…”

Section: Resultsmentioning

confidence: 98%

Development of Biomimetic Scaffolds with Both Intrafibrillar and Extrafibrillar Mineralization

Zhang

Wei

2015

ACS Biomater. Sci. Eng.

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Bone is an organic-inorganic hierarchical biocomposite. Its basic building block is mineralized collagen fibers with both intrafibrillar and extrafibrillar mineralization, which is believed to be regulated by non-collagenous proteins (NCPs) with polyanionic domains. In this study, collagen fibrils with both intrafibrillar and extrafibrillar mineralization were successfully prepared and the mechanism of biomineralization was proposed. Building on this success, a unique biomimetic lamellar scaffold composed of collagen fibrils with both intrafibrillar and extrafibrillar mineralization was fabricated using a combination of self-compression and unidirectional freeze drying approach. To achieve intrafibrillar mineralization, polyacrylic acid (PAA) was used to sequester calcium and phosphate ions to form fluidic PAA-amorphous calcium phosphate (PAA-ACP) nanoprecursors. At the presence of sodium tripolyphosphate (TPP), PAA-ACP nanoprecursors were modulated to orderly deposit within the gap zone of collagen fibrils. The effect of PAA concentration on the intrafibrillar and extrafibrillar mineralization of reconstituted collagen fibrils was investigated. It was found that with the decrease of PAA concentration, the inhibitory effect of PAA on mineralization and the stability of ACP nanoprecursors decreased. As a result, more minerals were deposited both within and on the surface of the collagen fibrils. Moreover, with the ability to reproduce biomineralization of collagen fibrils, it allowed us to fabricate a biomimetic hierarchical collagen/hydroxyapatite scaffolds composed of both intrafibrillar and extrafibrillar minerals using a bottom-up approach. This technique renders a promising biomimetic scaffold, which will be suitable for bone repair and regeneration.

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“…Similarly, other natural or synthetic proteins 18–26 and peptides 27–30 (e.g. salivary proteins such as mucins, statherin, and proline rich proteins) were reported to be able to bind on the tooth surface and protect the tooth from acid attack or aid in the remineralization of the tooth 18, 31, 32 .…”

Section: Introductionmentioning

confidence: 99%

“…salivary proteins such as mucins, statherin, and proline rich proteins) were reported to be able to bind on the tooth surface and protect the tooth from acid attack or aid in the remineralization of the tooth 18, 31, 32 . While proteins or peptides can avoid the negative effect, the costs of producing these materials are high 27, 33, 34 and they may have the risk of allergic reactions for certain populations. Therefore, it is of great importance to develop an alternative non-fluoride agent which is able to effectively protect the tooth against acid erosion and/or enhance tooth remineralization safely at low cost.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired amphiphilic phosphate block copolymers as non-fluoride materials to prevent dental erosion

et al. 2014

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Inspired by the fact that certain natural proteins, e.g. casein phosphopeptide or amelogenin, are able to prevent tooth erosion (mineral loss) and to enhance tooth remineralization, a synthetic amphiphilic diblock copolymer, containing a hydrophilic methacryloyloxyethyl phosphate block (MOEP) and a hydrophobic methyl methacrylate block (MMA), was designed as a novel non-fluoride agent to prevent tooth erosion under acidic conditions. The structure of the polymer, synthesized by reversible addition-fragment transfer (RAFT) polymerization, was confirmed by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance spectroscopy (NMR). While the hydrophilic PMOEP block within the amphiphilic block copolymer strongly binds to the enamel surface, the PMMA block forms a hydrophobic shell to prevent acid attack on tooth enamel, thus preventing/reducing acid erosion. The polymer treatment not only effectively decreased the mineral loss of hydroxyapatite (HAP) by 36–46% compared to the untreated control, but also protected the surface morphology of the enamel specimen following exposure to acid. Additionally, experimental results confirmed that low pH values and high polymer concentrations facilitate polymer binding. Thus, the preliminary data suggests that this new amphiphilic diblock copolymer has the potential to be used as a non-fluoride ingredient for mouth-rinse or toothpaste to prevent/reduce tooth erosion.

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In situ remineralizaiton of partially demineralized human dentine mediated by a biomimetic non-collagen peptide

Cited by 57 publications

References 35 publications

Fabrication of intrafibrillar and extrafibrillar mineralized collagen/apatite scaffolds with a hierarchical structure

Fabrication of intrafibrillar and extrafibrillar mineralized collagen/apatite scaffolds with a hierarchical structure

Development of Biomimetic Scaffolds with Both Intrafibrillar and Extrafibrillar Mineralization

Bioinspired amphiphilic phosphate block copolymers as non-fluoride materials to prevent dental erosion

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