In situ regulation of lipolysis by insulin and norepinephrine: A microdialysis study during euglycemic-hyperinsulinemic clamp

Cimmino, M; Agosto, A.; Minaire, Y; Géloën, Alain

doi:10.1016/0026-0495(95)90067-5

Cited by 14 publications

(10 citation statements)

References 19 publications

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“…With respect to regional differences in response to the antilipolytic action of insulin, the data from the present study support our previous findings (26,27) that femoral sc adipose tissue is more resistant to the effects of insulin than is abdominal sc adipose tissue. Femoral adipose tissue was more resistant to the antilipolytic action of insulin than was abdominal adipose tissue, which was more resistant than the whole body antilipolytic response to insulin, both before and after training.…”

Section: Regional Differences In the Antilipolytic Effect Of Insulin supporting

confidence: 91%

Effects of 10 Days of Endurance Exercise Training on the Suppression of Whole Body and Regional Lipolysis by Insulin1

Hickner

Racette

Binder

et al. 2000

The Journal of Clinical Endocrinology &Amp; Metabolism

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The aim of this study was to evaluate in premenopausal women (10 sedentary obese women) the effects of 10 days of exercise on the suppression of whole body and regional lipolysis by insulin. Lipolysis was determined using 2H5-glycerol infusion and microdialysis of sc adipose tissue during a two-stage hyperinsulinemic-euglycemic clamp [10 (LO) and 20 (MO) mU/m x min]. Microdialysis probes were positioned in abdominal and femoral sc adipose tissue to monitor interstitial glycerol and blood flow. Basal plasma glycerol was 86.7 +/-17.0 and 100.3 +/- 19.8 micromol/L before and after training, respectively (P < 0.05). Plasma glycerol was suppressed to a greater extent after [to 47 +/- 5% (LO) and 42 +/- 5% (MO) of basal] than before [to 62 +/- 8% (LO) and 55 +/- 8% (MO) of basal] training. The rate of appearance of glycerol was suppressed to 49 +/- 7% and 40 +/- 5% of basal during LO and to 38 +/- 5% and 30 +/- 4% of basal during MO (P < 0.05) before and after training, respectively. There were no differences in the suppression of lipolysis in abdominal as well as femoral sc adipose tissue as evidenced by similar reductions in dialysate glycerol levels before and after training in each of these tissues. The results indicate that the antilipolytic response to insulin can be improved through endurance exercise training. Intraabdominal adipose tissue or skeletal muscle may be the site of improved antilipolytic response to insulin after training, as improvement was not evident in abdominal or femoral sc adipose tissue.

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Section: Regional Differences In the Antilipolytic Effect Of Insulin supporting

confidence: 91%

Effects of 10 Days of Endurance Exercise Training on the Suppression of Whole Body and Regional Lipolysis by Insulin1

Hickner

Racette

Binder

et al. 2000

The Journal of Clinical Endocrinology &Amp; Metabolism

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“…Repeated intravenous perfusions of epinephrine (28) or microdialysis perfusion (24) could lead to desensitization of catecholamine-induced lipolysis in human adipose tissue. This phenomenon was not found in rat adipose tissue when norepinephrine was infused into microdialysis probes at 1-h intervals (8). In fact, it occurs in human adipose tissue when catecholamine perfusions are applied at intervals of 1 h (24) or less (28).…”

Section: Discussionmentioning

confidence: 94%

“…Simultaneously, insulin can be delivered intravenously during a hyperinsulinemic-euglycemic clamp with no changes of catecholamine concentration in plasma or in dialysate from adipose tissue or muscle (23,26). Animal studies have shown a decrease of the lipolytic response to norepinephrine during a hyperinsulinemic-euglycemic clamp in adipose tissue (8). From additional human investigations, it has been concluded that the ␤-adrenergic lipolytic effect in adipose tissue is counteracted by the action of insulin (14,18).…”

mentioning

confidence: 99%

Activation of α₂-adrenergic receptors blunts epinephrine-induced lipolysis in subcutaneous adipose tissue during a hyperinsulinemic euglycemic clamp in men

Štich

Pelikánová²,

Wohl³

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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. Activation of ␣2-adrenergic receptors blunts epinephrine-induced lipolysis in subcutaneous adipose tissue during a hyperinsulinemic euglycemic clamp in men. Am J Physiol Endocrinol Metab 285: E599-E607, 2003; 10.1152/ajpendo.00502.2002The aim of this study was to investigate whether hyperinsulinemia modifies adrenergic control of lipolysis, with particular attention paid to the involvement of antilipolytic ␣2-adrenergic receptors (AR). Eight healthy male subjects (age: 23.9 Ϯ 0.9 yr; body mass index: 23.8 Ϯ 1.9) were investigated during a 6-h euglycemichyperinsulinemic clamp and in control conditions. Before and during the clamp, the effect of graded perfusions of isoproterenol (0.1 and 1 M) or epinephrine (1 and 10 M) on the extracellular glycerol concentration in subcutaneous abdominal adipose tissue was evaluated by using the microdialysis method. Both isoproterenol and epinephrine induced a dosedependent increase in extracellular glycerol concentration when infused for 60 min through the microdialysis probes before and during hours 3 and 6 of the clamp. The catecholamine-induced increase was significantly lower during the clamp than before it, with the inhibition being more pronounced in hour 6 of the clamp. Isoproterenol (1 M)-induced lipolysis was reduced by 28 and 44% during hours 3 and 6 of the clamp, respectively, whereas the reduction of epinephrine (100 M)-induced lipolysis was significantly greater (by 63 and 70%, P Ͻ 0.01 and P Ͻ 0.04, respectively) during the same time intervals. When epinephrine was infused in combination with 100 M phentolamine (a nonselective ␣-AR antagonist), the inhibition of epinephrine (10 M)-induced lipolysis was only of 19 and 40% during hours 3 and 6 of the clamp, respectively. The results demonstrate that, in situ, insulin counteracts the epinephrine-induced lipolysis in adipose tissue. The effect involves 1) reduction of lipolysis stimulation mediated by the ␤-adrenergic pathway and 2) the antilipolytic component of epinephrine action mediated by ␣2-ARs. microdialysis; glycerol; isoproterenol; blood flow; ␣ 2-adrenergic receptor antagonist NOREPINEPHRINE AND EPINEPHRINE control human adipocyte lipolysis through different adrenergic receptor (AR) subtypes (1,7,20,30). In vitro studies in isolated human fat cells have shown that the activation of ␣ 2 -ARs by epinephrine and norepinephrine impairs the ␤-adrenergic component of catecholamine-induced lipolysis (19,20). In human fat cells, where ␣ 2 -ARs outnumber ␤-ARs, the preferential recruitment of the ␣ 2 -AR at the lowest catecholamine concentrations inhibits lipolysis (20). It is in subcutaneous adipose tissue from both men and women that the strongest ␣ 2 -adrenergic effect has been observed (20, 30). The antilipolytic action of catecholamines in vitro, particularly that of epinephrine (which exhibits a higher affinity for ␣ 2 -AR), is intense in subcutaneous adipocytes from obese subjects (20, 30). Many physiological and pathological studies have shown that the possible deregulation of adrenergic control in adipos...

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“…Insulin inhibits lipolysis and counters the action of adrenergic beta receptors by activating phosphodiesterase-3B, which hydrolyses cyclic AMP and also desensitises beta-adrenergic responsiveness [4]. Microdialysis allows the in situ study of the interaction of insulin with the adrenergic pathways in subcutaneous adipose tissue.…”

Section: Introductionmentioning

confidence: 99%

Dynamic strength training improves insulin sensitivity and functional balance between adrenergic alpha 2A and beta pathways in subcutaneous adipose tissue of obese subjects

et al. 2005

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In situ regulation of lipolysis by insulin and norepinephrine: A microdialysis study during euglycemic-hyperinsulinemic clamp

Cited by 14 publications

References 19 publications

Effects of 10 Days of Endurance Exercise Training on the Suppression of Whole Body and Regional Lipolysis by Insulin1

Effects of 10 Days of Endurance Exercise Training on the Suppression of Whole Body and Regional Lipolysis by Insulin1

Activation of α₂-adrenergic receptors blunts epinephrine-induced lipolysis in subcutaneous adipose tissue during a hyperinsulinemic euglycemic clamp in men

Dynamic strength training improves insulin sensitivity and functional balance between adrenergic alpha 2A and beta pathways in subcutaneous adipose tissue of obese subjects

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In situ regulation of lipolysis by insulin and norepinephrine: A microdialysis study during euglycemic-hyperinsulinemic clamp

Cited by 14 publications

References 19 publications

Effects of 10 Days of Endurance Exercise Training on the Suppression of Whole Body and Regional Lipolysis by Insulin1

Effects of 10 Days of Endurance Exercise Training on the Suppression of Whole Body and Regional Lipolysis by Insulin1

Activation of α2-adrenergic receptors blunts epinephrine-induced lipolysis in subcutaneous adipose tissue during a hyperinsulinemic euglycemic clamp in men

Dynamic strength training improves insulin sensitivity and functional balance between adrenergic alpha 2A and beta pathways in subcutaneous adipose tissue of obese subjects

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Activation of α₂-adrenergic receptors blunts epinephrine-induced lipolysis in subcutaneous adipose tissue during a hyperinsulinemic euglycemic clamp in men