In situ regeneration of NADH via lipoamide dehydrogenase-catalyzed electron transfer reaction evidenced by spectroelectrochemistry

Tam, Tsz Kin; Chen, Baowei; Lei, Chenghong; Li, Jun

doi:10.1016/j.bioelechem.2012.03.002

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…The UV-Vis spectra of the EPS at various potentials were measured using spectroelectrochemical analysis, which was performed using a thin-layer spectroelectrochemical cell with a Pt gauze working electrode, an Ag/AgCl reference electrode, and a Pt wire counter electrode36. The optical length of the spectroelectrochemical cell was 1 mm.…”

Section: Methodsmentioning

confidence: 99%

Redox properties of extracellular polymeric substances (EPS) from electroactive bacteria

Sheng

Cheng

et al. 2016

Sci Rep

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Although the capacity for electroactive bacteria to convert environmental metallic minerals and organic pollutants is well known, the role of the redox properties of microbial extracellular polymeric substances (EPS) in this process is poorly understood. In this work, the redox properties of EPS from two widely present electroactive bacterial strains (Shewanella oneidensis and Pseudomonas putida) were explored. Electrochemical analysis demonstrates that the EPS extracted from the two strains exhibited redox properties. Spectroelectrochemical and protein electrophoresis analyses indicate that the extracted EPS from S. oneidensis and P. putida contained heme-binding proteins, which were identified as the possible redox components in the EPS. The results of heme-mediated behavior of EPS may provide an insight into the important roles of EPS in electroactive bacteria to maximize their redox capability for biogeochemical cycling, environmental bioremediation and wastewater treatment.

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Section: Methodsmentioning

confidence: 99%

Redox properties of extracellular polymeric substances (EPS) from electroactive bacteria

Sheng

Cheng

et al. 2016

Sci Rep

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“…The regeneration typically takes place in a three‐reaction sequence (Figure 16), as was shown in the work of Chen et al., where synthesis of lactate from pyruvate was achieved in a membrane packed‐bed reactor [170] . Another instance of methyl viologen mediated electroreduction of NAD + with lipoamide dehydrogenase (LDH), also known as diaphorase, occurs at low cathodic overpotential [171] . In‐situ spectrophotometric study showed that the amount of NADH produced was incumbent on the LDH concentration while the rate of NADH regeneration depended on initial NAD + concentration and followed Michaelis–Menten kinetics.…”

Section: Electrochemical Regeneration Methodsmentioning

confidence: 93%

“…Similar to NAD(P) + reduction, direct electrochemical oxidation of NAD(P)H on a bare electrode also takes place at a high overpotential [17–178] . Cyclic voltammograms fro...…”

Section: Electrochemical Regeneration Methodsmentioning

confidence: 99%

“…[170] Another instance of methyl viologen mediated electroreduction of NAD + with lipoamide dehydrogenase (LDH), also known as diaphorase, occurs at low cathodic overpotential. [171] In-situ spectrophotometric study showed that the amount of NADH produced was incumbent on the LDH concentration while the rate of NADH regeneration depended on initial NAD + concentration and followed Michaelis-Menten kinetics. Diaphoraseviologen conjugate formed by covalent attachment has been featured in a recent work by Dinh et al, [172] which provides more flexible operational strategy as it can be separated from the reaction medium for further reuse.…”

Section: Indirect Electrochemical Regeneration Of 14-nadh Using Elect...mentioning

confidence: 99%

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Redox Biocatalysis: Quantitative Comparisons of Nicotinamide Cofactor Regeneration Methods

2022

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Enzymatic processes, particularly those capable of performing redox reactions, have recently been of growing research interest. Substrate specificity, optimal activity at mild temperatures, high selectivity, and yield are among the desirable characteristics of these oxidoreductase catalyzed reactions. Nicotinamide adenine dinucleotide (phosphate) or NAD(P)H‐dependent oxidoreductases have been extensively studied for their potential applications like biosynthesis of chiral organic compounds, construction of biosensors, and pollutant degradation. One of the main challenges associated with making these processes commercially viable is the regeneration of the expensive cofactors required by the enzymes. Numerous efforts have pursued enzymatic regeneration of NAD(P)H by coupling a substrate reduction with a complementary enzyme catalyzed oxidation of a co‐substrate. While offering excellent selectivity and high total turnover numbers, such processes involve complicated downstream product separation of a primary product from the coproducts and impurities. Alternative methods comprising chemical, electrochemical, and photochemical regeneration have been developed with the goal of enhanced efficiency and operational simplicity compared to enzymatic regeneration. Despite the goal, however, the literature rarely offers a meaningful comparison of the total turnover numbers for various regeneration methodologies. This comprehensive Review systematically discusses various methods of NAD(P)H cofactor regeneration and quantitatively compares performance across the numerous methods. Further, fundamental barriers to enhanced cofactor regeneration in the various methods are identified, and future opportunities are highlighted for improving the efficiency and sustainability of commercially viable oxidoreductase processes for practical implementation.

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“…Puesto que hasta hace poco no existía ningún dispositivo comercial para realizar medidas espectroelectroquímicas, han sido muchos los grupos que han utilizado este tipo de técnica multirrespuesta desarrollando sus propias celdas y dispositivos de medida. Las técnicas más utilizadas y para las que más desarrollos instrumentales de medida se han propuesto son la espectroelectroquímica [186][187][188] , las películas finas de oro 189,190 o las mallas metálicas de oro [191][192][193]…”

Section: Rayos X 166unclassified

New techniques and devices for UV-Vis absorption spectroelectrochemistry

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Hacen constar: Que la presente memoria resume el trabajo titulado "NEW TECHNIQUES AND DEVICES FOR UV-VIS SPECTROELECTROCHEMISTRY" realizado por D. Daniel Izquierdo Bote en el Área de Química Analítica y bajo nuestra dirección para optar al grado de Doctor por la Universidad de Burgos. Manifestamos nuestra conformidad con el contenido de la memoria y autorizamos su presentación para ser defendida como Tesis Doctoral. Burgos, 15 de septiembre de 2015. Fdo: Álvaro Colina Santamaría Fdo: Mª Aránzazu Heras Vidaurre IV V A mis padres VI VII AGRADECIMIENTOS Esta tesis puede verse como un resumen de mis investigaciones en estos últimos años. Sin embargo, no muestra más que una parte de lo vivido durante esta etapa, y este es el momento de dar las gracias a toda la gente que ha estado junto a mí durante todo este tiempo y que ha ayudado de una manera u otra a que esta tesis doctoral sea una realidad, porque sin su ayuda nunca podría haber tenido lugar. Me gustaría agradecer al Prof. Jesús López Palacios, pozo de sabiduría y humildad, y a mis directores de tesis, el Dr. Álvaro Colina y la Dra. Mª Aránzazu Heras, por su inestimable ayuda y por la confianza puesta en mí durante este tiempo. Pero especialmente, por todo lo que me han enseñado y por las experiencias vividas, tanto dentro del laboratorio entre potenciostatos y espectrofotómetros, como fuera de él, entre cañas y risas. Gracias por todo, especialmente por ayudarme a madurar y crecer tanto nivel científico como a nivel personal. No podría haber deseado mejores jefes/compañeros que ellos para compartir estos años. También quiero dar las gracias a mis argentinas, porque sin ellas esta tesis hubiera sido diferente y porque, gracias a sus complejos he aprendido que a veces las cosas son más complejas de lo que parecen. Gracias al lab-192, mi segunda casa durante este tiempo y por supuesto a sus moradores: David, Nola y Jesús a los que no puedo llamar compañeros, sino amigos. Aún recuerdo con cariño aquel día, en el que al fin empezaba la universidad. Quién me iba a decir ese día, en el aula 12 que después de tanto años, ese chico que estaba sentado a mi lado llamado David y una chica de unas filas VIII más allá, Nola, iban a acabar siendo mis compañeros de viaje. Gracias por estar ahí compartiendo innumerables e inolvidables momentos. Y gracias también a Jesús, ese compañero de escritorio con actitud protónica del que todos debiéramos aprender. Un momento importante durante estos años ha sido la hora del café, necesario algunos días para despejar la cabeza, mirar las cosas desde otra perspectiva o desconectar entre risas y bromas. Por todas estas cosas quiero agradecer a los analíticos: Julia, Hugo, Wil, Ana, Bego y Lorena. Gracias también a todos y cada uno de los compañeros del grupo de Análisis Instrumental y de toda el área de Química Analítica, por su ayuda y compañerismo. I would like to thank Prof. Wolfgang Schuhmann for giving me the opportunity of working in his research group in the Ruhr Universität Bochum. Thanks him and the rest of the group for their help...

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In situ regeneration of NADH via lipoamide dehydrogenase-catalyzed electron transfer reaction evidenced by spectroelectrochemistry

Cited by 9 publications

References 21 publications

Redox properties of extracellular polymeric substances (EPS) from electroactive bacteria

Redox properties of extracellular polymeric substances (EPS) from electroactive bacteria

Redox Biocatalysis: Quantitative Comparisons of Nicotinamide Cofactor Regeneration Methods

New techniques and devices for UV-Vis absorption spectroelectrochemistry

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