In situ probing reveals Aquabacterium commune as a widespread and highly abundant bacterial species in drinking water biofilms

Kalmbach, Sibylle; Manz, Werner; Bendinger, Bernd; Szewzyk, Ulrich

doi:10.1016/s0043-1354(99)00179-7

Cited by 69 publications

(37 citation statements)

References 17 publications

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“…Similar to other investigations for water supply systems using molecular methods (Kalmbach et al 2000;Schmeisser et al 2003;Williams et al 2004), the phylum Proteobacteria (especially Alphaproteobacteria or Betaproteobacteria) was the common and primary bacterial group in the biofilm communities from the two urban DWDSs. Within the Alphaproteobacteria, a number of Sphingomonadaceae-related sequences were retrieved from both Beijing and Guangzhou biofilms (Table 2).…”

Section: Discussionsupporting

confidence: 77%

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Liu

Zhang

et al. 2012

Can. J. Microbiol.

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In this study, to give insight into the bacterial diversity of biofilms from full-scale drinking water distribution systems (DWDSs), the bacterial community compositions of biofilms from two urban DWDSs (Guangzhou and Beijing, China) were determined using a 16S rRNA gene library technique. Meanwhile, the occurrence and diversity of mycobacteria were also analyzed by a Mycobacterium -specific hsp gene assay. The biofilms from the full-scale DWDSs have complex bacterial populations. Proteobacteria was the common and predominant group in all biofilm samples, in agreement with previous reports. The community structures of bacteria at the three sites in Guangzhou DWDS were significantly different, despite the similar physicochemical properties of portable water. Some abundant and peculiar bacterial phylotypes were noteworthy, including Methylophilus , Massilia , and Planomicrobium , members of which are rarely found in DWDSs and their roles in DWDS biofilms are still unclear. The diversity of Mycobacterium species in biofilm samples was rather low. Mycobacterium arupense and Mycobacterium gordonae were the primary Mycobacterium species in Guangzhou and Beijing biofilms, respectively, indicating that M. arupense may be more resistant to chloride than M. gordonae.

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Section: Discussionsupporting

confidence: 77%

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Liu

Zhang

et al. 2012

Can. J. Microbiol.

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“…Among the heterotrophic bacteria potentially supported by the aforementioned primary production, we identified diverse, abundant and prevalent OTUs classified to the family Comamonadaceae (Figures 4a and c). Previous studies identify related organisms predominating in DWDS-associated biofilms (Kalmbach et al, 1999;Kalmbach, 2000), faucet biofilms (Liu et al, 2012) and tap water Abbreviations: ANOSIM, analysis of similarities; DistLM, distance-based linear models; PERMANOVA, permutational analysis of variance.…”

Section: Discussionmentioning

confidence: 99%

Core-satellite populations and seasonality of water meter biofilms in a metropolitan drinking water distribution system

et al. 2015

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Drinking water distribution systems (DWDSs) harbor the microorganisms in biofilms and suspended communities, yet the diversity and spatiotemporal distribution have been studied mainly in the suspended communities. This study examined the diversity of biofilms in an urban DWDS, its relationship with suspended communities and its dynamics. The studied DWDS in Urbana, Illinois received conventionally treated and disinfected water sourced from the groundwater. Over a 2-year span, biomass were sampled from household water meters (n = 213) and tap water (n = 20) to represent biofilm and suspended communities, respectively. A positive correlation between operational taxonomic unit (OTU) abundance and occupancy was observed. Examined under a 'core-satellite' model, the biofilm community comprised 31 core populations that encompassed 76.7% of total 16 S rRNA gene pyrosequences. The biofilm communities shared with the suspended community highly abundant and prevalent OTUs, which related to methano-/methylotrophs (i.e., Methylophilaceae and Methylococcaceae) and aerobic heterotrophs (Sphingomonadaceae and Comamonadaceae), yet differed by specific core populations and lower diversity and evenness. Multivariate tests indicated seasonality as the main contributor to community structure variation. This pattern was resilient to annual change and correlated to the cyclic fluctuations of core populations. The findings of a distinctive biofilm community assemblage and methano-/methyltrophic primary production provide critical insights for developing more targeted water quality monitoring programs and treatment strategies for groundwater-sourced drinking water systems.

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“…It has been found to make up 2-53% of the cells found in drinking water system biofilms in Germany (Kalmbach et al, 2000), and Loy et al (2005) noted that they commonly make up a large portion of the microbial communities found in bottled natural mineral water. It made up 2.7% of the sequences from Canada.…”

Section: Discussionmentioning

confidence: 99%

Distantly sampled soils carry few species in common

et al. 2008

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The bacterial phylogenetic structure of soils from four distinctly different sites in South and North America was analyzed. One hundred and thirty-nine thousand sequences of the V9 region of the small subunit of the bacterial ribosomal RNA gene generated for a previous study were used for this work. Whereas the previous work estimated levels of species richness, this study details the degree of bacterial community overlap between the four soils. Sequences from the four soils were classified and grouped into different phyla and then assigned to operational taxonomic units (OTUs) as defined by 97 or 100% sequence similarity. Pairwise Jaccard and h similarity indices averaged over all phyla equalled 6 and 12% respectively at the 97% similarity level, and 15% for both at the 100% similarity level. At 100 and 97% sequence similarity, 1.5 and 4.1% of OTUs were found in all four soils respectively, and 87.9 and 74.4%, respectively were a unique particular soil. These analyses, based on the largest soil bacterial sequence retrieval to date, establish the high degree of community structure difference for randomly sampled dissimilar soils and support the idea that wide sampling is important for bioprospecting. The 10 most abundant cultured genera were determined in each soil. These 10 genera comprised a significant proportion of the reads obtained from each soil (31.3-37.4%). Chitinophaga was the most abundant or the second most abundant genus in all four soils with 7.5-13.8% of the total bacterial sequences in these soils. The striking result is that several culturable genera, whose roles in soil are virtually unknown, were found among these dominant sequences.

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In situ probing reveals Aquabacterium commune as a widespread and highly abundant bacterial species in drinking water biofilms

Cited by 69 publications

References 17 publications

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

Core-satellite populations and seasonality of water meter biofilms in a metropolitan drinking water distribution system

Distantly sampled soils carry few species in common

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