In Situ Polymerization of Methylene Blue on Bacterial Cellulose for Photodynamic/Photoelectricity Synergistic Inhibition of Bacterial Biofilm Formation

Abstract

In the context of long-term antimicrobial treatment, the emergence of bacterial resistance poses a significant challenge. Therefore, there is a pressing need to develop novel antimicrobial materials and methods that can effectively and safely combat microbial infections. This study focuses on the synthesis of bacterial cellulose-polymethylene blue (BC-PMB) with integrated photodynamic and photoelectric antimicrobial properties. The polymerization of methyl blue (MB) onto bacterial celluloses (BC) was achieved, and through comprehensive computational analyses using density functional theory (DFT) and molecular dynamics simulations, it was confirmed that this polymerization greatly enhanced the binding efficiency between methylene blue and BC. Additionally, polymethylene blue (PMB) exhibited superior photoexcitation efficiency and conductivity compared to its precursor. When BC-PMB was exposed to a 30 mW 660 nm light source for 30 min, the material demonstrated a remarkable antimicrobial efficacy of 93.99% against Escherichia coli and 98.58% against Staphylococcus aureus. Furthermore, the synergistic effect of photodynamic and photoelectric antimicrobial mechanisms exhibited long-term inhibitory capabilities against bacterial biofilms.

Advancing Photodynamic Antimicrobial Strategy: Sustainable Fabrication of Novel Lauryl Gallate-Chitosan Hydrophobic Films with Rapid Bacterial Capture and Biofilms Elimination Capabilities for Promoting Wound Healing

“…coli) and Staphylococcus aureus (S. aureus) . Roy’s group reported novel nanoparticles loaded with the PS, and with photodynamic properties, these nanoparticles can effectively eradicate S.…”