In situ photosynthetic yields of cave photoautotrophic biofilms using two different Pulse Amplitude Modulated fluorometers

Figueroa, Félix L.; Álvarez‐Gómez, Félix; Rosal, Yolanda del; Celis-Plá, Paula S. M.; Hernández, M.R.; Korbee, Nathalie

doi:10.1016/j.algal.2016.12.012

Cited by 12 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A portable fluorometer (Junior-PAM, Heinz Walz GmbH, Bavaria, Germany) was used to measure chlorophyll fluorescence parameters 18 . We selected rice leaves with consistent growth conditions, subjected to fully adapt to darkness for 30 min, and measured the maximum photochemical quantum yield of photosystem II (PS II) of the leaves, maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency (Y(II)), initial fluorescence (Fo), and non-photochemical quenching coefficient (NPQ).…”

Section: Methodsmentioning

confidence: 99%

Effect of silicon spraying on rice photosynthesis and antioxidant defense system on cadmium accumulation

Chen,

Huang,

Chen

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Cadmium (Cd) pollution is a serious threat to food safety and human health. Minimizing Cd uptake and enhancing Cd tolerance in plants are vital to improve crop yield and reduce hazardous effects to humans. In this study, we designed three Cd concentration stress treatments (Cd1: 0.20 mg·kg−1, Cd2: 0.60 mg·kg−1, and Cd3: 1.60 mg·kg−1) and two foliar silicon (Si) treatments (CK: no spraying of any material, and Si: foliar Si spraying) to conduct pot experiments on soil Cd stress. The results showed that spraying Si on the leaves reduced the Cd content in brown rice by 4.79–42.14%. Si application increased net photosynthetic rate (Pn) by 1.77–4.08%, stomatal conductance (Gs) by 5.27–23.43%, transpiration rate (Tr) by 2.99–20.50% and intercellular carbon dioxide (CO2) concentration (Ci) by 6.55–8.84%. Foliar spraying of Si significantly increased the activities of superoxide dismutase (SOD) and peroxidase (POD) in rice leaves by 9.84–14.09% and 4.69–53.09%, respectively, and reduced the content of malondialdehyde (MDA) by 7.83–48.72%. In summary, foliar Si spraying protects the photosynthesis and antioxidant system of rice canopy leaves, and is an effective method to reduce the Cd content in brown rice.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of silicon spraying on rice photosynthesis and antioxidant defense system on cadmium accumulation

Chen,

Huang,

Chen

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…where is the maximum fluorescence and is the minimal fluorescence, which is obtained when emitted a low intensity measuring light, which is measured momentarily before the saturation pulse is applied (Figueroa et al, 2017;Houliez et al, 2013;Le Rouzic, 2012). Phycocyanin detection was performed according to Bennet and Bogorad (1973), in which the water sample was concentrated in a centrifuge, the supernatant was removed, and the concentrated sample was frozen and thawed three times to break the cells.…”

Section: Pigment and Photosynthetic Activity Estimationmentioning

confidence: 99%

In Situ H2o2 Treatment of Blue-Green Algae Contaminated Reservoirs Causes Significant Improvement in Drinking Water Treatability

et al. 2022

View full text Add to dashboard Cite

“…Generally, microbial communities in cave entrances constitute biofilms ( Albertano, 2012 ), where there is also the presence of cyanobacteria and microalgae ( Poulíčková and Hasler, 2007 ; Czerwik-Marcinkowska, 2013 ; Lamprinou et al, 2014 ). Lampenflora typically causes biodeterioration of colonized surfaces ( Figueroa et al, 2017 ; Muñoz-Fernández et al, 2021 ), and a variety of approaches (i.e., physical, mechanical, and chemical) are utilized to limit photosynthesis and propagation ( Mulec and Kosi, 2009 ; Cigna, 2016 ; Figueroa et al, 2017 ). These strategies to devise the existence of lampenflora have been studied for many years.…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxybutyrate-producing cyanobacteria from lampenflora: The case study of the “Stiffe” caves in Italy

et al. 2022

View full text Add to dashboard Cite

This study aimed to estimate the green formation lampenflora of “Stiffe” caves in order to evaluate their suitability as an isolation source of cyanobacteria useful for the production of polyhydroxyalkanoates (PHAs). The cave system was chosen as the sampling site due to its touristic use and the presence of high-impact illuminations. The biofilms and the mats of the illuminated walls were sampled. Samples were investigated by 16S rRNA gene analysis and culturable cyanobacteria isolation. The isolated strains were then screened for the production of PHAs under typical culturing and nutritional starvation. Cultures were checked for PHA accumulation, poly-β-hydroxybutyrate (PHB) presence (infrared spectroscopy), and pigment production. The 16S rRNA gene metabarcoding. Highlighted a considerable extent of the pressure exerted by anthropogenic activities. However, the isolation yielded eleven cyanobacteria isolates with good PHA (mainly PHB)-producing abilities and interesting pigment production rates (chlorophyll a and carotenoids). Under normal conditions (BG110), the accumulation abilities ranged from 266 to 1,152 ng mg dry biomass–1. The optimization of bioprocesses through nutritional starvation resulted in a 2.5-fold increase. Fourier transform infrared (FTIR) studies established the occurrence of PHB within PHAs extracted by cyanobacteria isolates. The comparison of results with standard strains underlined good production rates. For C2 and C8 strains, PHA accumulation rates under starvation were higher than Azospirillum brasilense and similar to Synechocystis cf. salina 192. This study broadened the knowledge of the microbial communities of mats and biofilms on the lightened walls of the caves. These findings suggested that these structures, which are common in tourist caves, could be used to isolate valuable strains before remediation measures are adopted.

show abstract

In situ photosynthetic yields of cave photoautotrophic biofilms using two different Pulse Amplitude Modulated fluorometers

Cited by 12 publications

References 46 publications

Effect of silicon spraying on rice photosynthesis and antioxidant defense system on cadmium accumulation

Effect of silicon spraying on rice photosynthesis and antioxidant defense system on cadmium accumulation

In Situ H2o2 Treatment of Blue-Green Algae Contaminated Reservoirs Causes Significant Improvement in Drinking Water Treatability

Polyhydroxybutyrate-producing cyanobacteria from lampenflora: The case study of the “Stiffe” caves in Italy

Contact Info

Product

Resources

About