In situ phenol removal from fed-batch fermentations of solvent tolerant Pseudomonas putida S12 by pertraction

Heerema, Louise; Wierckx, Nick; Roelands, Mark; Hanemaaijer, J.H.; Goetheer, Earl; Verdoes, D.; Keurentjes, J.T.F.

doi:10.1016/j.bej.2010.11.002

Cited by 20 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the toxicity of phenol could be reduced by in situ product removal, the presence of octanol substantially extended the fermentation time, consequently reducing the productivity [3,4]. The final strain P. putida S12TPL3 produced 142 and 471 mg/L of phenol in flask and fed-batch cultures, respectively.…”

Section: Research Articlementioning

confidence: 99%

Metabolic engineering ofEscherichia colifor the production of phenol from glucose

Kim

Park

et al. 2013

Biotechnology Journal

View full text Add to dashboard Cite

Phenol is an industrially versatile commodity chemical and is currently produced from fossil resources. Phenol's biological production from renewable resources has been limited due to its toxicity to microorganisms. Here, we simultaneously engineered 18 Escherichia coli strains for the production of phenol using synthetic regulatory small RNA (sRNA) technology. sRNA-based knock-down of the two regulators and overexpression of the genes involved in the tyrosine biosynthetic pathway together with tyrosine phenol-lyase (TPL) in E. coli strains resulted in the production of phenol from glucose. The 18 engineered E. coli strains showed significant differences in the production of tyrosine (i.e. the immediate precursor for phenol), TPL activity, and tolerance to phenol. Among the engineered E. coli strains, the BL21 strain produced phenol most efficiently: 419 mg/L by flask culture and 1.69 g/L by fed-batch culture. The final titer and productivity were further improved through biphasic fed-batch fermentation using glycerol tributyrate as an extractant of phenol. The concentration of phenol in the glycerol tributyrate phase and fermentation broth reached 9.84 and 0.3 g/L, respectively, in 21 hours, which translates into the final phenol titer and productivity of 3.79 g/L and 0.18 g/L/h, respectively. This is the highest titer achieved by microbial fermentation. Although further engineering is required to be competitive with the current petro-based process, the strategies used for the development of the engineered strain and fermentation process will provide a valuable framework for the microbial production of toxic chemicals.

show abstract

Section: Research Articlementioning

confidence: 99%

Metabolic engineering ofEscherichia colifor the production of phenol from glucose

Kim

Park

et al. 2013

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…Higher overall E. coli cell growth rate Membrane based processes Lactic acid production Continuous removal of lactic acid from (Pal et al, 2009) fermentation broth Fermentation is carried out in a continuous mode Higher productivity Reactive crystallization Efficient calcium lactate production by Higher average productivity (Xu and Xu, 2014) Fermentation coupled with crystallization-based in-situ product removal Membrane absorption Recovery and purification of biofuel compounds, Economic recovery (Nielsen et al, 2010) including ethanol, iso-propanol, n-propanol, iso-butanol, n-butanol, 2 methyl-1-butanol, 3-methyl-1-butanol and n-pentanol Liquid-liquid extraction In-situ product removal of phenol from P. Putida Increased yields and productivity (Heerema et al, 2011;S12TPL fermentations López-Garzón and Recovery of fermentative carboxylic acids Straathof, 2014) Ultrasound Shikonin production in L. Erythrorhizon Enhance shikonin production in cell cultures, (Cai et al, 2012) cell cultures increased extraction yield from 20 to 65-70%, due partially to an increase in the cell membrane permeability by sonication …”

Section: Energymentioning

confidence: 99%

Process Intensification for Production and Recovery of Biological Products

Vaghari¹,

Eskandari²,

Sobhani³

et al. 2015

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Bioprocesses are important biological reactions which need several sophisticated methods and equipment to produce many novel and important compounds which some of them are traditionally produced by synthetic chemical reactions. In bioprocesses, the products are often produced in a dilute environment and finally they require a high purity. Because of that, downstream processes are usually included a large number of separation steps. Size and capital costs of the equipment are two main limitations of using bioprocesses at industrial scale. Bioprocess intensification by minimizing, substitution, moderation and simplification of the methods and equipment, drastically leads to sustainable processes. This study looks at intensification of the emerging equipment and operational methods and their advantages to lead smaller and cleaner bioprocess plants which in turn, increases production efficiency and quality and decreases byproducts formation, capital cost and energy consumption.

show abstract

“…The maximum production rate was estimated from several references that use similar bacteria [5,8,9]. Additionally, c ph,b corresponds to the concentration of phenol in the reactor or bulk (mol m −3 ), see also Fig.…”

Section: Simulation Of the Phenol Concentration Profile In The Reactomentioning

confidence: 99%

Evaluation of an integrated extraction process for in-situ phenol removal with micellar solutions of PEO-PPO-PEO block copolymers

Heerema

Cakali

Roelands

et al. 2010

Separation and Purification Technology

Self Cite

View full text Add to dashboard Cite

In situ phenol removal from fed-batch fermentations of solvent tolerant Pseudomonas putida S12 by pertraction

Cited by 20 publications

References 45 publications

Metabolic engineering ofEscherichia colifor the production of phenol from glucose

Metabolic engineering ofEscherichia colifor the production of phenol from glucose

Process Intensification for Production and Recovery of Biological Products

Evaluation of an integrated extraction process for in-situ phenol removal with micellar solutions of PEO-PPO-PEO block copolymers

Contact Info

Product

Resources

About